Chicken Protein Hydrolysates Have Anti-Inflammatory Effects on High-Fat Diet Induced Obesity in Mice

Abstract

Background: Studies have shown that dietary source of protein and peptides can affect energy metabolism and influence obesity-associated diseases. This study aimed to investigate the impact of different chicken protein hydrolysates (CPHs) generated from chicken rest raw materials in a mouse obesity model. Methods: Male C57BL/6 mice were fed a high-fat, high-sucrose diet with casein or CPHs generated using Papain + Bromelain, Alcalase, Corolase PP, or Protamex for 12 weeks (n = 12). Body weight, feed intake, and intraperitoneal glucose tolerance was determined, and plasma and liver and adipose tissues were collected at sacrifice. Results: The average feed intake and body weight did not differ between the groups and white adipose tissue depots were unchanged, except for a reduction in the subcutaneous depot in mice fed the Protamex CPH diet. Moreover, the CPH diets did not prevent increased fasting glucose and insulin levels. Interestingly, the hepatic mitochondrial fatty acid β-oxidation was increased in mice fed Alcalase and Corolase PP CPHs. All CPH diets reduced plasma interleukine (IL)-1β, interferon-γ, tumor necrosis factor α, and monocyte chemotactic protein 1 compared to control, indicating anti-inflammatory effects. In addition, Corolase PP and Protamex CPHs significantly reduced plasma levels of IL-1α, IL-2, IL-6, IL-10, and granulocyte macrophage colony-stimulating factor. Conclusions: CPH diets were not able to counteract obesity and glucose intolerance in a mouse obesity model, but strongly reduced inflammatory parameters associated with obesity. Alcalase and Corolase PP CPHs also stimulated mitochondrial fatty acid β-oxidation. The possibility that hydrolysates from chicken rest raw materials could alleviate obesity-associated metabolic disease should be investigated further.

A chicken protein hydrolysate exerts anti‐atherosclerotic effect beyond plasma cholesterol‐lowering activity in Apoe−/− mice

“…Corolase PP generated protein hydrolysates that both affected the hepatic mitochondrial fatty acid oxidation in mice, while Corolase PP CPH had the strongest anti-inflammatory potential (Aloysius et al, 2018). The data presented here show that after 12 weeks of high- Significant difference between groups was determined using one-way ANOVA with Dunnett's multiple comparisons test (*p < .05) compared to control (Figure 1a,b).…”

“…We have recently shown that a protein hydrolysate from salmon reduced atherosclerosis in Apoe −/− mice, most likely through effects on systemic inflammation (Parolini et al, 2014). Based on previous findings in mice, the amount and type of dietary protein and/or hydrolysates may influence fatty acid oxidation, hypolipidemia, and inflammation (Aloysius et al, 2018;Vik et al, 2015). Therefore, it was of interest to investigate a potential anti-atherosclerotic effect of protein hydrolysates from chicken previously shown to reduce plasma cytokine levels in a mouse obesity model (Aloysius et al, 2018).…”

“…of the casein was replaced with CPH generated using the food grade commercial enzyme Corolase PP. The CPHs were generated as previously published (Aloysius et al, 2018).…”

“…In this study, we investigated the anti-atherosclerotic potential of two chicken protein hydrolysates, previously shown to increase mitochondrial fatty acid oxidation and reduce systemic inflammation (Aloysius et al, 2018), on atherosclerotic development in apolipoprotein E knockout (Apoe −/− ) mice, characterized by hyperlipidaemia and susceptibility to atherosclerosis (Zhang, Reddick, Piedrahita, & Maeda, 1992).…”

Effects of Smoking on the Level of Lipase Enzyme and Lipid Profile in Blood Serum of Young Smokers

“…These negatively charge fatty acids, once released in the bloodstream gets bind to the positively charged calcium ions. This process of salt formation between negatively charged fatty acids and positively charged calcium ions is called saponification 9 . The present study aim to evaluate the effects of smoking on the lipid profile and lipase levels among Iraqi smokers as well as to identify the morbidity risk among smokers and non-smokers.…”