Chicken skin-derived collagen peptides chelated zinc promotes zinc absorption and represses tumor growth and invasion in vivo by suppressing autophagy

Abstract

To improve the utilization value of chicken by-products, we utilized the method of step-by-step hydrolysis with bromelain and flavourzyme to prepare low molecular weight chicken skin collagen peptides (CCP) (<5 kDa) and characterized the amino acids composition of the CCP. Then, we prepared novel CCP-chelated zinc (CCP–Zn) by chelating the CCP with ZnSO4. We found that the bioavailability of CCP–Zn is higher than ZnSO4. Besides, CCP, ZnSO4, or CCP–Zn effectively repressed the tumor growth, invasion, and migration in a Drosophila malignant tumor model. Moreover, the anti-tumor activity of CCP–Zn is higher than CCP or ZnSO4. Furthermore, the functional mechanism studies indicated that CCP, ZnSO4, or CCP–Zn inhibits tumor progression by reducing the autonomous and non-autonomous autophagy in tumor cells and the microenvironment. Therefore, this research provides in vivo evidence for utilizing chicken skin in the development of zinc supplements and cancer treatment in the future.

Dietary zinc supplements: beneficial health effects and application in food, medicine and animals

“…Its antioxidant activity was either preserved or boosted when oyster protein hydrolysate was chelated with zinc 95 . Apart from that, peptide zinc chelate also has biological activities such as enhancing anticancer cell proliferation 96 and suppressing autophagy to prevent in vivo tumor growth and invasion, 97 and so forth. The number of zinc‐chelating peptides that are commercially available is small because much of their manufacture and analysis are still being done in laboratories.…”

Developments for collagen hydrolysates as a multifunctional antioxidant in biomedical domains

“…It explains why oxidative stress is detrimental to human health. When oxidative stress occurs, the balance of the redox system is damaged, resulting in the accumulation of a large number of intracellular oxidation products, which may be associated with accelerated aging, neurodegeneration, inflammation, tumor, diabetes, and other diseases [3][4][5][6][7].…”