Background: India consistently reports the most diphtheria, pertussis, tetanus and measles cases worldwide. Persistent exposure to fecal pathogens due to open defecation may cause environmental enteropathy that in may lead to undernutrition and vaccine failure in under 5-year old (u5) children. The Swachh Bharat Mission (SBM) program in India, launched in 2014, aimed to construct toilets for every household nationwide and reduce open defecation. Objective: We examine whether increased household toilet availability in Indian districts corresponds with a reduction in 4 Vaccine Preventable Diseases (VPDs): diphtheria, pertussis, tetanus and measles.Methods: We retrieved data on district level change in the prevalence (per 1000 u5 children) of 4 VPDs, from 2013 (pre-SBM) to 2016 (post-SBM). We obtained data on our exposure, the change in the percentage of households with toilets (per district), from three large national surveys conducted in 2013 and 2016. We used linear regression analysis which controlled for change over time in socioeconomic factors, health system-related covariates and pre-SBM prevalence of VPDs.Results: A one percentage point increase in households with toilets corresponds with 0.33 fewer measles cases per 1000 u5 children in that district (coefficient: -0.33, 95% CI: -0.0641, -0.014; p < 0.05). We observe no relation with diphtheria, pertussis, or tetanus. Conclusion: Rapid improvements in ambient sanitation through increased toilet availability (and reduction in open defecation) may correspond with reduction in the prevalence of measles in u5 children.