In this work, kindergarten toy storage, defined as the construction area, puzzle area, scientific observation area, and role-playing area, and the toy characteristics of the four areas, was studied. Interviews and grounded theory were used to observe and summarize the behavioral needs of 3- to 6-year-old children and preschool teachers. Analytical hierarchy process (AHP) was used to analyze behavioral needs. It was concluded that the kindergarten toy locker optimization was designed to improve storage efficiency. However, the current layout of kindergarten toy lockers is chaotic, and children cannot efficiently and autonomously take toys from toy lockers. The best toy locker layout scheme was selected through an eye tracking experiment. The subjects were all 3- to 6-year-old children, a total of 30 people. By comparing the data such as hot spot map, trajectory map, area of the first viewpoint, and gaze time when children observed different lockers layout during the experiment, the optimal layout scheme of kindergarten toy lockers was comprehensively analyzed. Optimizing the zoning, classification, and storage of kindergarten toys is conducive to improving the efficiency of children’s independent storage, creating a kindergarten game and teaching environment conducive to children’s development.