Chilling injury in human kidney tubule cells after subzero storage is not mitigated by antifreeze protein addition

Tomalty, Heather E.; Graham, Laurie A.; Walker, Virginia K.; Davies, Peter L.

doi:10.1016/j.cryobiol.2023.05.002

Cited by 2 publications

(1 citation statement)

References 73 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In vitro , immortalized human proximal-tubule epithelial cells stored at −6°C for up to 6 days with antifreeze proteins or cryostasis storage solution showed decreased viability, increased lactate dehydrogenase (LDH) release, and apoptosis compared to 4°C storage in UW solution. This suggests that this kidney cell type might be prone to chilling injury and that the addition of antifreeze proteins and cryostasis may not be effective for increasing storage times at subzero temperatures ( 42 ). However, it was demonstrated that preservation of neonatal rat kidneys at −2°C for 48 h in histidine-tryptophan-ketoglutarate (HTK) solution at −2°C reduced histological damage (cortex and medulla) compared to 24 or 4°C ( 43 ).…”

Section: Supercooling Principles To Extend Organ Preservationmentioning

confidence: 99%

Supercooling: a promising technique for prolonged preservation in solid organ transplantation, and early perspectives in vascularized composite allografts

Berkane,

Hayau,

Filz von Reiterdank

et al. 2023

Front. Transplant.

View full text Add to dashboard Cite

Ex vivo preservation of transplanted organs is undergoing spectacular advances. Machine perfusion is now used in common practice for abdominal and thoracic organ transportation and preservation, and early results are in favor of substantially improved outcomes. It is based on decreasing ischemia-reperfusion phenomena by providing physiological or sub-physiological conditions until transplantation. Alternatively, supercooling techniques involving static preservation at negative temperatures while avoiding ice formation have shown encouraging results in solid organs. Here, the rationale is to decrease the organ's metabolism and need for oxygen and nutrients, allowing for extended preservation durations. The aim of this work is to review all advances of supercooling in transplantation, browsing the literature for each organ. A specific objective was also to study the initial evidence, the prospects, and potential applications of supercooling preservation in Vascularized Composite Allotransplantation (VCA). This complex entity needs a substantial effort to improve long-term outcomes, marked by chronic rejection. Improving preservation techniques is critical to ensure the favorable evolution of VCAs, and supercooling techniques could greatly participate in these advances.

show abstract

Section: Supercooling Principles To Extend Organ Preservationmentioning

confidence: 99%