Chilling of Steindachneridion parahybae (siluriformes: pimelodidae) embryos

Lopes, Taís da Silva; Sanches, Eduardo Antônio; Okawara, Renan Yoshiharu; Romagosa, Elizabeth

doi:10.1016/j.theriogenology.2015.04.005

Cited by 8 publications

(3 citation statements)

References 21 publications

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“…It is also important to test new cryoprotectants with the same characteristics. Methanol has properties that are common among short-chain alcohols, such as the ability to lower the phase transition temperature of the lipid membrane; and this mechanism of protection reported positive results for the cryopreservation of fish embryos (LOPES et al, 2012(LOPES et al, , 2015. Solutions of PG promoted small and amorphous ice formation due to their high viscosity at temperatures below zero.…”

Section: Discussionmentioning

confidence: 99%

Surubim-do-Paraíba oocytes viability after being exposed to different cryoprotectants

et al. 2018

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To know the non-toxic cryoprotectants to fish oocytes is of extreme importance for tests that aim to increase oocyte resistance to cold, thus allowing more advanced studies in cryopreservation. Therefore, commonly used cryoprotectants such as methanol, dimethyl sulfoxide, ethylene glycol, propylene glycol, sucrose and fructose were studied. Immature oocytes from the initial to vitelogenic (diameter <1.7 mm) and mature (diameter >1.8 mm) stages of Steindachneridion parahybae were evaluated. Four distinct experiments were performed, three using immature oocytes and one using oocytes at the mature stage. For each oocyte stage, the best maintenance solution to be used: Hank or 50% L15 and; viability after baths for 30min (room temperature) at cryoprotectant concentrations ranging from 0.25 to 4M were evaluated. Different tests were used to evaluate oocyte viability: in vitro maturation followed by observation of germinal vesicle breakdown (only for immature oocytes), Trypan Blue staining (all stages) and fertilization and hatching rates (mature stage only). Results showed that the toxic effect of cryoprotectants on oocytes generally increases with increasing concentrations. Sensitivity of oocytes to cryoprotectants increases according to the stage of development, with mature oocytes being more sensitive. Sucrose, fructose, methanol, propylene glycol and dimethyl sulfoxide can be used as cryoprotectants for S. parahybae oocytes.

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Section: Discussionmentioning

confidence: 99%

Surubim-do-Paraíba oocytes viability after being exposed to different cryoprotectants

et al. 2018

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“…Beberapa studi tentang kriopreservasi embrio telah dilakukan pada beberapa spesies ikan (Ahammad et al, 2002(Ahammad et al, , 2003Xiao et al, 2008;Lin et al, 2009a;Lin et al, 2009b;Shaluei et al, 2013;Sharifuddin dan Azizah, 2014;Lopes et al, 2015;Tian et al, 2015) akan tetapi, belum menunjukkan hasil yang maksimal.…”

Section: Pendahuluanunclassified

Studi Pendahuluan: Pengaruh Krioprotektan yang Berbeda terhadap Persentase Kerusakan dan Penetasan Embrio Ikan Lele Dumbo (Clarias gariepinus)

Mukti‬

Eka

Satyantini

et al. 2020

JAFH

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Tujuan penelitian ini adalah mengetahui pengaruh krioprotektan yang berbeda dalam kriopreservasi embrio terhadap persentase kerusakan dan penetasan embrio ikan lele dumbo (Clarias gariepinus). Embrio ikan lele pada fase gastrula diberi perlakuan larutan dimethyl sulfoxide (DMSO), propylene glycol (PG), madu, dan kombinasinya dengan konsentrasi 5% untuk masing-masing perlakuan. Embrio disimpan pada suhu 4 dan 0ºC, masing-masing selama 30 menit, 1, 2, 3, 4, 5, dan 6 jam. Thawing embrio dilakukan pada suhu 28ºC. Selanjutnya, embrio diinkubasi dalam akuarium pada suhu 28ºC. Hasil penelitian menunjukkan bahwa perbedaan krioprotektan dalam kriopreservasi embrio fase gastrula memberikan perbedaan kerusakan dan penetasan embrio ikan lele pada lama waktu penyimpanan yang berbeda. Persentase kerusakan embrio meningkat seiring dengan lama waktu penyimpanan dan suhu penyimpanan yang berbeda. Kombinasi larutan krioprotektan DMSO dan madu serta PG dan madu menunjukkan persentase kerusakan embrio yang lebih rendah dan persentase penetasan embrio yang lebih tinggi dibandingkan perlakuan krioprotektan lainnya (p<0,05).

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“…This company had supported research on the biology of S. parahybae, especially on reproductive physiology and development biology. Studies on S. parahybae aimed mainly to establish of a protocol for artificial spawning [3,4], develop embryos and larvae [5][6][7], improve broodstock metabolism [8] and the neuroendocrine system [9][10][11], and improve the quality of gametes of S. parahybae [6,[11][12][13].…”

Section: Introductionmentioning

confidence: 99%

Arginine-Vasotocin Neuronal System in Steindachneridion parahybae (Siluriformes: Pimelodidae) and Its Influence on Artificially Induced Spawning in Captivity

Honji,

Araújo,

de Mello

et al. 2024

Fishes

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This study summarizes new data on induced spawning of Steindachneridion parahybae, focusing on the aggressive behavior of females. This study characterizes the vasotocinergic system using immunohistochemistry, highlighting the potential influence of arginine-vasotocin (AVT) on reproductive physiology. Two experimental groups were proposed: (A) control, with one female in the aquarium, and (B) experimental, with two females in the same aquarium. Dominant (D) females presented a more aggressive behavior and did not show any injury. They apparently had a length and body mass higher than injured nondominant (ND) females. The analysis identified positive AVT immunoreactive (ir) neurons exclusively within the preoptic area, including parvocellular, magnocellular, and gigantocellular subpopulations, containing fibers-ir extending into the pituitary gland. Cellular and nuclear areas were greater in D compared to ND in the magnocellular subpopulation. There were no differences between parvocellular and gigantocellular subpopulations. There was a difference on the steroid plasma profile of cortisol (more in ND than in D) and 17α,20β-dihydroxy-4-pregnen-3-one (more in D than in ND). Furthermore, control and D females presented higher optical densities for AVT-ir, gonadotropin-releasing hormone-ir, and luteinizing hormone-ir than ND. In general, there were no differences in the results of female (control group) with D females. The AVT system is highly complex, possibly counting multiple sites of action during artificial reproduction and acting directly and/or indirectly associated with behavioral and physiological changes in S. parahybae females when induced to spawning.

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Chilling of Steindachneridion parahybae (siluriformes: pimelodidae) embryos

Cited by 8 publications

References 21 publications

Surubim-do-Paraíba oocytes viability after being exposed to different cryoprotectants

Surubim-do-Paraíba oocytes viability after being exposed to different cryoprotectants

Studi Pendahuluan: Pengaruh Krioprotektan yang Berbeda terhadap Persentase Kerusakan dan Penetasan Embrio Ikan Lele Dumbo (Clarias gariepinus)

Arginine-Vasotocin Neuronal System in Steindachneridion parahybae (Siluriformes: Pimelodidae) and Its Influence on Artificially Induced Spawning in Captivity

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