2013
DOI: 10.5935/1981-2965.20130027
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Chilling of tambaqui (Colossoma macropomum) embryos at different times of storage

Abstract: ABSTRACT:The technique of cryopreservation of fish embryos has not efficient results yet, however, the cooling of embryos has been used with satisfactory results. The aim of this study was cooling embryos Colossoma macropomum subjected to a temperature of 2ºC at different times of storage. Embryos were used in the following embryonic stages: blastoderm -1.2 hours post-fertilization (hpf), epibolia -5-hpf; closing blastopore -8-hpf; appearance of the optic vesicle -13 hpf. One hundred embryos, of each stage of … Show more

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Cited by 3 publications
(2 citation statements)
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“…Reviews of cryopreservation of semen of Brazilian freshwater fish, including tambaqui, have been published by Viveiros & Godinho 176 and Garcia et al 177 Although protocols for cryopreservation of tambaqui semen are established, those for embryos and oocytes still lack good results 178 . A cooling protocol for tambaqui embryos kept up to 8 h at 2°C has been suggested for embryos at the stages of blastospore closure (8 h post‐fertilization) and appearance of the optical vesicle (13 h post‐fertilization) 139 …”
Section: Reproduction and Hatcherymentioning
confidence: 99%
“…Reviews of cryopreservation of semen of Brazilian freshwater fish, including tambaqui, have been published by Viveiros & Godinho 176 and Garcia et al 177 Although protocols for cryopreservation of tambaqui semen are established, those for embryos and oocytes still lack good results 178 . A cooling protocol for tambaqui embryos kept up to 8 h at 2°C has been suggested for embryos at the stages of blastospore closure (8 h post‐fertilization) and appearance of the optical vesicle (13 h post‐fertilization) 139 …”
Section: Reproduction and Hatcherymentioning
confidence: 99%
“…Studies on reproduction in C. macropomum have focused on cryopreservation of gametes (Varela et al, 2012), cooling of embryos (Pessoa et al, 2013), evaluation of different hormonal inducing regimens (Martins et al, 2017), adequate inseminating doses (Leite et al, 2013), assessment of gamete quality (Maria et al, 2010), and successive semen collections (Pires et al, 2017). There, however, is no scientific information on successive oocyte collections in C. macropomum in the same reproductive period.…”
Section: Introductionmentioning
confidence: 99%