Chilling, oxidative stress and antioxidant responses inArabidopsis thaliana callus

O'Kane, Damian; Gill, Vera; Boyd, Patricia A.; Burdon, Roy H.

doi:10.1007/bf00620053

Cited by 239 publications

(138 citation statements)

References 25 publications

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“…5B). Other general responses to cold-induced membrane damage are an increased accumulation of cryoprotectants (12,13,15); LEA, cold-and heat-shock proteins (HSP), which participate in membrane protection and refolding of denatured proteins (88,89); and the accumulation of antioxidants mostly at chloroplast level (90,91).…”

Section: Discussionmentioning

confidence: 99%

Systemic Cold Stress Adaptation of Chlamydomonas reinhardtii

Valledor

Furuhashi

Hanak

et al. 2013

Molecular & Cellular Proteomics

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Chlamydomonas reinhardtii is one of the most important model organisms nowadays phylogenetically situated between higher plants and animals (Merchant et al. 2007). Stress adaptation of this unicellular model algae is in the focus because of its relevance to biomass and biofuel production. Here, we have studied cold stress adaptation of C. reinhardtii hitherto not described for this algae whereas intensively studied in higher plants. Toward this goal, high throughput mass spectrometry was employed to integrate proteome, metabolome, physiological and cell-morphological changes during a time-course from 0 to 120 h. These data were complemented with RT-qPCR for target genes involved in central metabolism, signaling, and lipid biosynthesis. Using this approach dynamics in central metabolism were linked to cold-stress dependent sugar and autophagy pathways as well as novel genes in C. reinhardtii such as CKIN1, CKIN2 and a hitherto functionally not annotated protein named CKIN3. Cold stress affected extensively the physiology and the organization of the cell. Gluconeogenesis and starch biosynthesis pathways are activated leading to a pronounced starch and sugar accumulation. Quantitative lipid profiles indicate a sharp decrease in the lipophilic fraction and an increase in polyunsaturated fatty acids suggesting this as a mechanism of maintaining membrane fluidity. The proteome is completely remodeled during cold stress: specific candidates of the ribosome and the spliceosome indicate altered biosynthesis and degradation of proteins important for adaptation to low temperatures. Specific proteasome degradation may be mediated by the observed cold-specific changes in the ubiquitinylation system. Sparse partial least squares regression analysis was applied for protein correlation network analysis using proteins as predictors and Fv/Fm, FW, total lipids, and starch as responses. We applied also Granger causality analysis and revealed correlations between proteins and metabolites otherwise not detectable. Twenty percent of the proteins responsive to cold are uncharacterized proteins. This presents a considerable resource for new discoveries in cold stress biology in alga and plants. Molecular &

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Section: Discussionmentioning

confidence: 99%

Systemic Cold Stress Adaptation of Chlamydomonas reinhardtii

Valledor

Furuhashi

Hanak

et al. 2013

Molecular & Cellular Proteomics

130

131

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“…Because of their function in the ascorbate-GSH pathway, the contribution of GSH and GR to protecting plants against chilling-induced oxidative stress was previously discussed in several publica- tions (Badiani et al, 1993;Walker and McKersie, 1993;Kocsy et al, 1996;1997;2000b;O'Kane et al, 1996;Alscher et al, 1997;Foyer et al, 1997;Noctor and Foyer, 1998;Zhao and Blumwald, 1998;Leipner et al, 1999). The biphasic relationship between GR activity or TG and the relative protection of maize from chilling-induced injury presented here is an important new finding.…”

Section: Discussionmentioning

confidence: 99%

“…An increase in the total glutathione (TG) level in white pine during winter (Anderson et al, 1992) and with increasing altitude in alpine plants (Wildi and Lü tz, 1996) was explained by assuming that GSH was used as an antioxidant to protect against low temperature-induced injuries. This assumption was supported by studies carried out under controlled conditions in which cold treatment increased the TG content (Vierheller and Smith, 1990;Brunner et al, 1995;O'Kane et al, 1996;Badiani et al, 1997;Zhao and Blumwald, 1998;KingstonSmith et al, 1999). At low, nonfreezing temperatures, the GSH content and GSH to GSSG ratio were higher in tolerant genotypes of tomato, Sorghum bicolor, and wheat compared with sensitive ones (Walker and McKersie, 1993;Badiani et al, 1997;Kocsy et al, 2000a), indicating that the maintenance of a high GSH to GSSG ratio contributes to improved chilling tolerance or cold hardening.…”

mentioning

confidence: 87%

“…The GR activity increased correspondingly during winter in Picea abies growing in the field (Esterbauer and Grill, 1978). In growth chamber experiments, low temperature treatment increased the GR activity in the leaves of spinach (de Kok and Oosterhuis, 1983), in the roots of jack pine (Zhao and Blumwald, 1998), and in Arabidopsis callus (O'Kane et al, 1996). The induction of an increase in GR activity by paclobutrazol in a chilling-sensitive maize (Zea mays) genotype was accompanied by better chilling tolerance (Pinhero et al, 1997).…”

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confidence: 99%

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Increasing the Glutathione Content in a Chilling-Sensitive Maize Genotype Using Safeners Increased Protection against Chilling-Induced Injury

et al. 2001

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With the aim of analyzing their protective function against chilling-induced injury, the pools of glutathione and its precursors, cysteine (Cys) and ␥-glutamyl-Cys, were increased in the chilling-sensitive maize (Zea mays) inbred line Penjalinan using a combination of two herbicide safeners. Compared with the controls, the greatest increase in the pool size of the three thiols was detected in the shoots and roots when both safeners were applied at a concentration of 5 m. This combination increased the relative protection from chilling from 50% to 75%. It is interesting that this increase in the total glutathione (TG) level was accompanied by a rise in glutathione reductase (GR; EC 1.6.4.2) activity. When the most effective safener combination was applied simultaneously with increasing concentrations of buthionine sulfoximine, a specific inhibitor of glutathione synthesis, the total ␥-glutamyl-Cys and TG contents and GR activity were decreased to very low levels and relative protection was lowered from 75% to 44%. During chilling, the ratio of reduced to oxidized thiols first decreased independently of the treatments, but increased again to the initial value in safener-treated seedlings after 7 d at 5°C. Taking all results together resulted in a linear relationship between TG and GR and a biphasic relationship between relative protection and GR or TG, thus demonstrating the relevance of the glutathione levels in protecting maize against chilling-induced injury.

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“…Initially, antioxidative capacity can mitigate the potentially damaging effects of ROS signaling occurring during low-temperature response (O'Kane et al, 1996;Suzuki and Mittler, 2006). Antioxidative proteins are also involved in the recovery phase after stress (Biemelt et al, 1998;Blokhina et al, 2003).…”

Section: Antioxidative and Detoxification Proteinsmentioning

confidence: 99%

Proteomic Study of Low-Temperature Responses in Strawberry Cultivars (Fragaria×ananassa) That Differ in Cold Tolerance

Koehler

Wilson²,

Goodpaster

et al. 2012

Plant Physiology

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To gain insight into the molecular basis contributing to overwintering hardiness, a comprehensive proteomic analysis comparing crowns of octoploid strawberry (Fragaria 3 ananassa) cultivars that differ in freezing tolerance was conducted. Four cultivars were examined for freeze tolerance and the most cold-tolerant cultivar ('Jonsok') and least-tolerant cultivar ('Frida') were compared with a goal to reveal how freezing tolerance is achieved in this distinctive overwintering structure and to identify potential cold-tolerance-associated biomarkers. Supported by univariate and multivariate analysis, a total of 63 spots from twodimensional electrophoresis analysis and 135 proteins from label-free quantitative proteomics were identified as significantly differentially expressed in crown tissue from the two strawberry cultivars exposed to 0-, 2-, and 42-d cold treatment. Proteins identified as cold-tolerance-associated included molecular chaperones, antioxidants/detoxifying enzymes, metabolic enzymes, pathogenesis-related proteins, and flavonoid pathway proteins. A number of proteins were newly identified as associated with cold tolerance. Distinctive mechanisms for cold tolerance were characterized for two cultivars. In particular, the 'Frida' cold response emphasized proteins specific to flavonoid biosynthesis, while the more freezing-tolerant 'Jonsok' had a more comprehensive suite of known stress-responsive proteins including those involved in antioxidation, detoxification, and disease resistance. The molecular basis for 'Jonsok'-enhanced cold tolerance can be explained by the constitutive level of a number of proteins that provide a physiological stress-tolerant poise. Strawberry (Fragaria 3 ananassa) cultivation predominates in regions with mild winters. In colder climates, overwintering hardiness is an essential trait for strawberry cultivation. Freezing injury of strawberry plants is one of the greatest factors in reducing crop yield and quality in temperate regions. Winter damage in Norway, for example, on average causes losses of 20%. Thus, production of cultivars with improved freezing hardiness is one of Norway's major objectives for their strawberry breeding programs. Improvement of cold hardiness is desirable for securing economic sustainability of the existing crops, and for expanding the growing regions of temperate fruit crops. Because strawberry is a representative species for the Rosaceae crops (e

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Chilling, oxidative stress and antioxidant responses inArabidopsis thaliana callus

Cited by 239 publications

References 25 publications

Systemic Cold Stress Adaptation of Chlamydomonas reinhardtii

Systemic Cold Stress Adaptation of Chlamydomonas reinhardtii

Increasing the Glutathione Content in a Chilling-Sensitive Maize Genotype Using Safeners Increased Protection against Chilling-Induced Injury

Proteomic Study of Low-Temperature Responses in Strawberry Cultivars (Fragaria×ananassa) That Differ in Cold Tolerance

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