Chilling requirement for breaking dormancy and flowering in Paeonia lactiflora ‘Taebaek’ and ‘Mulsurae’

Rhie, Yong Ha; Jung, Hyun Hwan; Kim, Ki Sun

doi:10.1007/s13580-012-0002-x

Cited by 24 publications

(19 citation statements)

References 13 publications

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“…When the day length and temperature decrease between August and October in autumn, the buds appear to enter a stage of endodormancy, but eventually shift to a stage of ecodormancy under cold stress in December and January for over-wintering in China. Most large buds develop into aboveground parts again in the spring with rise in temperature[ 1 , 8 – 10 ].…”

Section: Introductionmentioning

confidence: 99%

Transcriptomic Analysis of the Underground Renewal Buds during Dormancy Transition and Release in ‘Hangbaishao’ Peony (Paeonia lactiflora)

Zhang

et al. 2015

PLoS ONE

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Paeonia lactiflora is one of the most famous species of herbaceous peonies with gorgeous flowers. Bud dormancy is a crucial developmental process that allows P. lactiflora to survive unfavorable environmental conditions. However, little information is available on the molecular mechanism of the bud dormancy in P. lactiflora. We performed de novo transcriptome sequencing using the Illumina RNA sequencing platform for the underground renewal buds of P. lactiflora ‘Hangbaishao’ to study the molecular mechanism underlying its bud dormancy transition (the period from endodormancy to ecodormancy) and release (the period from ecodormancy to bud elongation and sprouting). Approximately 300 million high-quality clean reads were generated and assembled into 207,827 (mean length = 828 bp) and 51,481 (mean length = 1250 bp) unigenes using two assembly methods named “Trinity” and “Trinity+PRICE”, respectively. Based on the data obtained by the latter method, 32,316 unigenes were annotated by BLAST against various databases. Approximately 1,251 putative transcription factors were obtained, of which the largest number of unique transcripts belonged to the basic helix-loop-helix protein (bHLH) transcription factor family, and five of the top ten highly expressed transcripts were annotated as dehydrin (DHN). A total of 17,705 simple sequence repeat (SSR) motifs distributed in 13,797 sequences were obtained. The budbreak morphology, levels of indole-3-acetic acid (IAA) and abscisic acid (ABA), and activities of guaiacol peroxidase (POD) and catalase (CAT) were observed. The expression of 20 interested unigenes, which annotated as DHN, heat shock protein (HSP), histone, late elongated hypocotyl (LHY), and phytochrome (PHY), and so on, were also analyzed. These studies were based on morphological, physiological, biochemical, and molecular levels and provide comprehensive insight into the mechanism of dormancy transition and release in P. lactiflora. Transcriptome dataset can be highly valuable for future investigation on gene expression networks in P. lactiflora as well as research on dormancy in other non-model perennial horticultural crops of commercial significance.

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Section: Introductionmentioning

confidence: 99%

Transcriptomic Analysis of the Underground Renewal Buds during Dormancy Transition and Release in ‘Hangbaishao’ Peony (Paeonia lactiflora)

Zhang

et al. 2015

PLoS ONE

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“…The optimum temperature and duration of cold treatment of peony varieties also varies. Kamenetsky et al (2003) suggested that the best treatment to release dormancy of P. lactiflora 'Sarah Bernhardt' was 2°C for 60 d or 6°C for 70 d. Rhie et al (2012) found that chilling for 6 weeks at 0 and 9 weeks at 5°C was needed to break dormancy and to induce flowering in P. lactiflora 'Taebaek' and 'Mulsurae', respectively. Thus, the effectiveness of temperatures must be determined for the regulation of flowering for annual commercial production (Dole, 2003;Park et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

Comprehensive Application of Different Methods of Observation Provides New Insight into Flower Bud Differentiation of Double-flowered Paeonia lactiflora ‘Dafugui’

Zhang¹,

Zhu²,

Silva³

et al. 2019

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Additional index words. bud development, flower type, herbaceous peony, observation methods of bud differentiationAbstract. The analysis of herbaceous peony (Paeonia lactiflora Pall.) floral bud initiation and differentiation from autumn to early spring is important because flower formation is strictly correlated with flowering in late spring. A clear understanding of peony flower development would allow management practices to be optimized for stable annual production and supply of cut flowers. The aim of this research was to study the timesensitive characteristics underlying flower development in peony cultivar 'Dafugui'. Flower bud differentiation was assessed by paraffin and hand-made slices, as well as stereomicroscopy. Both methods have strengths and weaknesses. Our observations are not entirely consistent with those of previous studies. Flower bud differentiation in 'Dafugui', which starts from late September to mid-April of the following year, is divided into differentiation of the lower parts (separate differentiation periods for the bracts, sepals, petals, stamens, and pistillodes) and upper parts (separate differentiation periods for the petals, stamens, and pistil), and is closely related to annual changes in ambient temperature. Differentiation of lower flower buds takes place before winter dormancy, whereas that of upper parts starts early in the following spring, and finally forms a double-flowered type. The three methods used in this article can be used to study P. lactiflora flower bud differentiation and can contribute to an understanding of this process in the future.

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“…External environmental factors, short-day and low temperature, play an essential role in endodormancy induction, and su cient low temperature accumulation is a necessary prerequisite to bud break [3][4][5]. In Paeonia lacti ora, low temperature accumulation at 5℃ for nine weeks was required to ensure the re-growth of buds [6]. The optimal temperatures for bud dormancy release in apple and sweet cherry were − 2 °C to 5.5 °C and − 2 °C to 7 °C, respectively, which was determined by temperature control experiments [7].…”

Section: Introductionmentioning

confidence: 99%

Metabonomic analysis reveals EMP pathway activation and flavonoid accumulation during dormancy transition in tree peony

Zhang

Yuan

Zhan

et al. 2020

Preprint

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Background Bud dormancy is a sophisticated strategy in which plants evolved to survive in tough environments. Chilling exposure is an effective method to promote dormancy release in perennial plants including tree peony, and endodormancy is a key obstacle for forcing culture of tree peony. However, the mechanism of dormancy release is still poorly understood, and there are few systematic studies on the metabolomics during chilling induced dormancy transition. Results A total of 535 small molecules were obtained in tree peony buds treated with chilling, belonging to flavonoids, amino acid and its derivatives, lipids, organic acids and its derivates, nucleotide and its derivates, alkaloids, hydroxycinnamoyl derivatives, carbohydrates and alcohols, phytohormones, coumarins, and vitamins, respectively. 118 differential metabolites were detected, and they involved in serveral metabolic pathways related to dormancy. Sucrose was the most abundant carbohydrate in peony bud. Starch degradation and EMP activity were enhanced during the dormancy release, according to the variations of sugar contents, related enzyme activities and key genes expression. Flavonoids synthesis and accunmulation were also promoted by prolonged chilling, which might attribute to the activated expression of PsDFR and PsANS. Moreover, the variations of phytohormones (SA, JA, ABA, and IAA) indicated they played different roles in dormancy transition. Conclusion Our study suggested that starch degradation, EMP activation, and flavonoid accumulation were crucial and associated with bud dormancy transition in tree peony.

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Chilling requirement for breaking dormancy and flowering in Paeonia lactiflora ‘Taebaek’ and ‘Mulsurae’

Cited by 24 publications

References 13 publications

Transcriptomic Analysis of the Underground Renewal Buds during Dormancy Transition and Release in ‘Hangbaishao’ Peony (Paeonia lactiflora)

Transcriptomic Analysis of the Underground Renewal Buds during Dormancy Transition and Release in ‘Hangbaishao’ Peony (Paeonia lactiflora)

Comprehensive Application of Different Methods of Observation Provides New Insight into Flower Bud Differentiation of Double-flowered Paeonia lactiflora ‘Dafugui’

Metabonomic analysis reveals EMP pathway activation and flavonoid accumulation during dormancy transition in tree peony

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