Chimeric plumage coloration produced by ovarian transplantation in chickens

Liu, J.; Robertson, M. C.; Cheng, Kimberly M.; Silversides, F. G.

doi:10.3382/ps.2012-02843

Cited by 6 publications

(7 citation statements)

References 19 publications

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“…A vitrification method that has been used in Japanese quail (Liu et al, 2013b) and chickens (Liu et al, 2013c) was adapted to ovaries from post-hatching turkey pouts with minimal modifications. Ovaries were put into fresh HM at room temperature (RT) and four ovaries (from four different poults) were transfixed on an acupuncture needle (J-type, Size No.…”

Section: Vitrification and Warming Proceduresmentioning

confidence: 99%

“…Techniques available to mammals, such as cryobanking of oocytes or embryos, have limited applicability for birds because of the structure of the avian egg ( Rothchild, 2003 ; Hagedorn, 2006 ). Alternatively, the ovarian tissue can be cryopreserved and recovered by orthotopic transplantation, which has been achieved in Japanese quail and chickens ( Liu et al , 2010 ; 2013b , c ) and used to preserve specialized lines in these species ( Silversides et al , 2013 ). The essentiality of extrapolating this model to the domestic turkey, another important poultry species and model organism ( Moore and Siopes, 2004 ; Aslam et al , 2012 ), appears obvious.…”

Section: Introductionmentioning

confidence: 99%

“…For all poultry species studied to date, the age of the donor birds ranged from day-old (chicken) to week-old (quail) ( Liu et al , 2010 ; 2013b , c ), taking advantage of the potentially superior cryosurvival of immature oocytes compared to mature oocytes ( Woods et al , 2004 ). The effect of vitrification procedures on cryosurvival of ovarian tissue at different developmental stages in turkeys is not known.…”

Section: Introductionmentioning

confidence: 99%

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Microscopic Morphology and Apoptosis of Ovarian Tissue after Cryopreservation using a Vitrification Method in Post-Hatching Turkey Poults, <i>Meleagris gallopavo</i>

2017

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The objectives of this study were to examine morphological changes of oogonia and primordial follicles in the ovaries of turkey poults within the first week after hatching, and to evaluate the effect of cryopreservation on histology and apoptosis of these immature ovaries. Ovaries from poults at Day 1, Day 3, Day 5 and Day 7 post-hatch were cryopreserved using a modified vitrification method. The histology of oogonia and primordial follicles in fresh and cryopreserved tissue was assessed, and the apoptosis of tissue in different age groups was identified using a terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay. The mean oogonium diameter in fresh tissue increased from 11.9±1.3 μm (Day 1) to 15.2±2.7 μm (Day 7) within the first week; however, oogonia in cryopreserved tissue from Day 3 and Day 7 ovaries were smaller than those in fresh tissue (P＜0.05). Formation of primordial follicles was observed as early as Day 5. For Day 7 ovaries, follicles in cryopreserved tissue were smaller than those in fresh tissue; this was also true for oocyte diameter (P＜0.05). Apoptosis was most frequent in Day 1 fresh tissue, which was reduced as the poults aged. The frequency of apoptosis in cryopreserved tissue was comparable among age groups. This study provides the first documentation of morphological changes in the turkey ovary within the first week post-hatching. Results suggest that oogonia and primordial follicles that are smaller in size could be more resistant to the damage caused by cryopreservation. Of the ages assessed in this study, it is concluded that 3 days of age appears optimal for recovery of donor ovaries for cryopreservation, taking the advance of reduced cryoinjury and ease of tissue handling at this age.

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Section: Vitrification and Warming Proceduresmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Microscopic Morphology and Apoptosis of Ovarian Tissue after Cryopreservation using a Vitrification Method in Post-Hatching Turkey Poults, <i>Meleagris gallopavo</i>

2017

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“…Surgical procedures used for ovariectomy of 1-day-old chickens [20] were adapted for castration of 1-wk-old quail. Recipient WB chicks were anesthetized by administration of isoflurane gas.…”

Section: Testicular Allograftingmentioning

confidence: 99%

Production of Live Offspring from Testicular Tissue Cryopreserved by Vitrification Procedures in Japanese Quail (Coturnix japonica)1

Liu

Cheng

Silversides

2013

Biology of Reproduction

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Cryopreservation of testicular tissue can be used for ex situ conservation of male germplasm of avian species. The possibility of using vitrification and transplantation of testicular tissue for fertility preservation and recovery was tested in Japanese quail. Testes were removed from 1-wk-old Japanese quail; transfixed on acupuncture needles; equilibrated with dimethyl sulphoxide, ethylene glycol, and sucrose; plunged into liquid nitrogen; and stored in 2-ml straws. Cryopreserved tissue was warmed in sucrose solution at room temperature or at 40°C. Fresh and cryopreserved tissue were transplanted subcutaneously into castrated, 1-wk-old recipients. Twenty of 21 recipients survived the surgery, and 18 had viable transplants at maturity, with no difference in transplantation success between fresh and cryopreserved tissue. Fluid extrusion from 11 of the transplants was collected and inseminated surgically into the magnum of 22 quail hens, and 10 inseminations included foam from the proctodeal gland of the same recipients. Egg production in the 2 wk after insemination was reduced, and none of the hens inseminated with foam produced fertile eggs. Five hens inseminated without foam produced a total of eight live offspring; four of these hens had been inseminated with fluid extrusion from cryopreserved tissue. Histological examination showed spermatogenesis in the transplants, and the tubules, lumens, and epithelium of the seminiferous tubules were of comparable size to those of testicular tissue from intact males. These results demonstrate that testicular tissue of Japanese quail can be preserved using vitrification procedures and recovered through transplantation.

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“…This technique has been successfully used to produce live offspring from fresh ovarian transplants in chickens (Song and Silversides, 2007b), Japanese quail (Song and Silversides, 2008a) and ducks (Song et al, 2012) and from cryopreserved ovarian transplants in Japanese quail (Liu et al, 2010). We have recently improved this technique by making a smaller cut parallel to the median plane on the left side of the abdominal cavity of the recipients to expose the ovary without externalising the gizzard and intestines (Liu et al, 2013a). In addition, Karagenç et al (2011) demonstrated that survival of two-day old recipients is better than one-day old recipients and we now conduct the transplantation in older chicks which significantly reduces mortality during surgery and simplifies the technique by eliminating the need to remove the yolk sac (unpublished data).…”

Section: Transplantation Of Avian Gonadal Tissuementioning

confidence: 99%

Fundamental Principles of Cryobiology and Application to Ex situ Conservation of Avian Species

Liu

Cheng

Silversides

2013

Avian Biology Research

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Cryopreservation of animal germplasm is an important ex situ conservation strategy. Germplasm can be preserved at subzero temperatures and recovered in a way that allows germline development to be resumed at a later time. This strategy is routinely used for genetic improvement of dairy cattle, maintenance of lab strains of rodents and in human reproductive medication, and it contributes to genetic conservation of a growing number of wild mammalian species. Although there is an urgent need, cryoconservation of germplasm for birds is limited, partly because of skepticism with respect to the effectiveness of cryopreservation techniques. An understanding of fundamental principles of cryobiology and comparative cryobiological and physiological properties of germplasm is essential to the development and optimisation of cryopreservation protocols. In mammals, slow freezing has been used to cryopreserve gametes and embryos but the effectiveness of slow freezing is specific to the cell type and it does not protect extracellular structures. Vitrification, which is a process of solidification without crystallisation, can be used to effectively preserve multicellular structures such as gonadal tissue. In some domestic and wild avian species, the fertility produced by cryopreserved semen is sufficient for conservation but the w chromosome is lost. Avian eggs and embryos cannot be cryopreserved, but gonadal tissue can be cryopreserved and recovered by transplantation. Cryopreservation of germplasm allows ex situ conservation of avian species because it allows repopulation without the limitations of geographic isolation or reproductive longevity.

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Chimeric plumage coloration produced by ovarian transplantation in chickens

Cited by 6 publications

References 19 publications

Microscopic Morphology and Apoptosis of Ovarian Tissue after Cryopreservation using a Vitrification Method in Post-Hatching Turkey Poults, <i>Meleagris gallopavo</i>

Microscopic Morphology and Apoptosis of Ovarian Tissue after Cryopreservation using a Vitrification Method in Post-Hatching Turkey Poults, <i>Meleagris gallopavo</i>

Production of Live Offspring from Testicular Tissue Cryopreserved by Vitrification Procedures in Japanese Quail (Coturnix japonica)1

Fundamental Principles of Cryobiology and Application to Ex situ Conservation of Avian Species

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