Chinese goose (Anser cygnoides) CD8a: Cloning, tissue distribution and immunobiological in splenic mononuclear cells

Zhao, Qiurong; Liu, Fei; Chen, Shun; Yan, Xiaoling; Qi, Yu; Wang, Mingshu; Jia, Renyong; Zhu, Dekang; Liu, Mafeng; Chen, Xiaoyue; Cheng, Anchun

doi:10.1016/j.gene.2013.07.104

Cited by 8 publications

(7 citation statements)

References 40 publications

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“…In addition, CD8+ T cells mainly produce cytokines such as interferon-γ (IFN-γ) and tumornecrosisfactor (TNF)-α, which further activate macrophages and inhibit viral replication . In summary, CD8 is a marker of CD8+ T cells, which are expressed on the surface of cells and are involved in the clearance of viruses, antigen presentation and the immune response (Zhao et al 2013).…”

Section: Introductionmentioning

confidence: 99%

“…However, the CD8 antibody used in these ELISA kits was based on human CD8 or chicken CD8, not goose CD8. Based on the goose cDNA sequence that we previously characterized (Zhao et al 2013), we developed a method for the specific detection of goose CD8α. Two polyclonal antibodies (PAbs) were obtained based on goose CD8α (goCD8α), and in light of this, we established a DAS-ELISA to specifically detect goCD8α.…”

Section: Introductionmentioning

confidence: 99%

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Development and optimization of a double antibody sandwich ELISA for the detection of goose T cell surface CD8α molecule

Zhang

Cheng

Chen

et al. 2016

Journal of Integrative Agriculture

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CD8, a glycoprotein on the surface of T cells, is involved in the defense against viral infection and plays significant roles in antigen presentation and in the antiviral immune response. CD8 is composed of two chains. Of these, the CD8α chain was chosen for the detection because it involved in both the CD8αα homodimer and the CD8αβ heterodimer. Here, we established a double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) for specific detection of goose CD8α (goCD8α). The results showed that the optimal coated antibody and antigen dilutions were 1:50 (the antibody titer was 1:12 800) and 1:32 (0.3 ng mL-1), respectively, while the optimal capture antibody and horseradish peroxidase (HRP)-labelled goat anti-rabbit IgG dilutions were 1:50 (the antibody titer was 1:51 200) and 1:4 000 (the antibody titer was 1:5 000), respectively. The optimal blocking buffer was 5% bovine serum albumin (BSA). The best incubating condition was overnight at 4°C, the best blocking time was 120 min and the best anti-capture antibody working time was 150 min. In addition, the minimum dose detectable by DAS-ELISA was 5×10-3 ng mL-1. Most importantly, goCD8α expression levels in goose spleen mononuclear cells (MNCs) post-Goose parvoviruse (GPV) infection were found to be significantly up-regulated using the DAS-ELISA method, which was consistent with previous results obtained using real-time quantitative PCR. In conclusion, the DAS-ELISA method reported here is a novel, specific technique for the clinical detection of goCD8α.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Development and optimization of a double antibody sandwich ELISA for the detection of goose T cell surface CD8α molecule

Zhang

Cheng

Chen

et al. 2016

Journal of Integrative Agriculture

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“…Therefore, it is essential to illustrate the nature and regulation of the innate and adaptive immune systems in the goose [ 5 ]. However, except chicken and other poultry such as goose, relatively little is known about their immune systems at the molecular level [ 6 ]. Thus, the discovery of important immune genes and functional studies can help elucidate immunological responses and the natural or inherited disease resistance ability.…”

Section: Introductionmentioning

confidence: 99%

De Novo Transcriptomic Analysis of Peripheral Blood Lymphocytes from the Chinese Goose: Gene Discovery and Immune System Pathway Description

et al. 2015

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BackgroundThe Chinese goose is one of the most economically important poultry birds and is a natural reservoir for many avian viruses. However, the nature and regulation of the innate and adaptive immune systems of this waterfowl species are not completely understood due to limited information on the goose genome. Recently, transcriptome sequencing technology was applied in the genomic studies focused on novel gene discovery. Thus, this study described the transcriptome of the goose peripheral blood lymphocytes to identify immunity relevant genes.Principal Findings De novo transcriptome assembly of the goose peripheral blood lymphocytes was sequenced by Illumina-Solexa technology. In total, 211,198 unigenes were assembled from the 69.36 million cleaned reads. The average length, N50 size and the maximum length of the assembled unigenes were 687 bp, 1,298 bp and 18,992 bp, respectively. A total of 36,854 unigenes showed similarity by BLAST search against the NCBI non-redundant (Nr) protein database. For functional classification, 163,161 unigenes were comprised of three Gene Ontology (Go) categories and 67 subcategories. A total of 15,334 unigenes were annotated into 25 eukaryotic orthologous groups (KOGs) categories. Kyoto Encyclopedia of Genes and Genomes (KEGG) database annotated 39,585 unigenes into six biological functional groups and 308 pathways. Among the 2,757 unigenes that participated in the 15 immune system KEGG pathways, 125 of the most important immune relevant genes were summarized and analyzed by STRING analysis to identify gene interactions and relationships. Moreover, 10 genes were confirmed by PCR and analyzed. Of these 125 unigenes, 109 unigenes, approximately 87%, were not previously identified in the goose.ConclusionThis de novo transcriptome analysis could provide important Chinese goose sequence information and highlights the value of new gene discovery, pathways investigation and immune system gene identification, and comparison with other avian species as useful tools to understand the goose immune system.

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“…Aquatic birds are regarded as the natural reservior for many viruses such as avian influenza virus (AIV), and play an important role in the transmission and dissemination of pathogens (Urbaniak et al, 2014). In our previously researches, the cDNA sequences of goose CD4 and CD8␣ were cloned, and the immune biological characters of them were identified Zhao et al, 2013). However, the tissue distribution profile and the ontogeny of T lymphocytes in goose is still scarce and fragmentary.…”

Section: Discussionmentioning

confidence: 99%

Age-related development and tissue distribution of T cell markers (CD4 and CD8a) in Chinese goose

et al. 2015

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Chinese goose (Anser cygnoides) CD8a: Cloning, tissue distribution and immunobiological in splenic mononuclear cells

Cited by 8 publications

References 40 publications

Development and optimization of a double antibody sandwich ELISA for the detection of goose T cell surface CD8α molecule

Development and optimization of a double antibody sandwich ELISA for the detection of goose T cell surface CD8α molecule

De Novo Transcriptomic Analysis of Peripheral Blood Lymphocytes from the Chinese Goose: Gene Discovery and Immune System Pathway Description

Age-related development and tissue distribution of T cell markers (CD4 and CD8a) in Chinese goose

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