Chinese herbal medicine for immune infertility: A protocol for systematic review and meta-analysis

Cui, Jiang; Wang, Zhaodi; Yuan, Shiqing; Jiang, Yong; Ye, Ying

doi:10.37766/inplasy2020.12.0073

Cited by 1 publication

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“…Therefore, we selected dydrogesterone as the control medicine in this experiment. Chinese medicine emphasizes the idea of “prevention before disease onset [ 21 ].” The treatment of RSA focuses on “compensating weakness in advance” before the pregnancy, especially the kidneys and spleen, and protecting the fetus as soon as possible after the pregnancy occurs [ 22 , 23 ]. This concept is similar to that of preventive medicine in Western medicine [ 24 ].…”

Section: Discussionmentioning

confidence: 99%

Shoutai Wan Improves Embryo Survival by Regulating Aerobic Glycolysis of Trophoblast Cells in a Mouse Model of Recurrent Spontaneous Abortion

Liang

Tang

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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Background. During embryo implantation, the blastocyst exhibits a high capacity for aerobic glycolysis, which results in a unique microenvironment of high lactate/low pH at the maternal-fetal interface. Shoutai Wan (STW) is an effective Chinese herbal formula widely used in the clinical treatment of recurrent spontaneous abortion (RSA). However, the specific molecular mechanism by which STW prevents abortion is yet to be elucidated. Methods. Female CBA/J mice were allocated into six groups randomly and then mated with BALB/c mice as the control group, DBA/2 mice as the RSA model, CBA/J×DBA/2 mice treated with dydrogesterone as the DQYT group, or CBA/J×DBA/2 mice treated with low, medium, and high-dose STW as the STW-L, STW-M, and STW-H groups, respectively. Drug administration started 14 days before mating and ended on the 14th day of pregnancy. The embryo loss rate of each group was calculated on day 14 of gestation, and the pregnancy outcomes of the mice in each group were observed. The mouse serum was collected to determine the levels of progesterone (P) and chorionic gonadotropin (CG). The activities of HK2, PKM2, and LDHA, the key glycolytic enzymes in each group, were detected. The expressions of lactate, ATP, HK2, PKM2, LDHA, MCT4, GLUT1, and GPR81 as well as the morphology of trophoblast cells were examined. Results. The embryo loss rate and adverse pregnancy outcomes were significantly increased ( P < 0.05 ) in the RSA model group. After dydrogesterone or different doses of STW treatment, the embryo loss rate and adverse pregnancy outcomes were rescued to varying degrees ( P < 0.05 ). Interestingly, there was no significant difference among the groups in terms of serum P and CG ( P < 0.05 ). Moreover, the activities of key glycolytic enzymes, lactate, ATP, HK2, PKM2, LDHA, MCT4, GLUT1, GPR81 protein or mRNA expression, and morphological abnormalities of trophoblast cells improved significantly in the RSA mice after dydrogesterone or different doses of STW treatment ( P < 0.05 ). Conclusion. STW can promote aerobic glycolysis in trophoblast cells of RSA mouse embryos, thereby improving the microenvironment of the maternal-fetal interface and enhancing embryo implantation.

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Section: Discussionmentioning

confidence: 99%