Chinese Medicine Leptochloa chinensis Inhibits the Malignant Behaviors of Renal Cell Carcinoma 786-O Cells by Regulating the mTOR Pathway

Abstract

Background. Renal cell carcinoma (RCC) is a common malignant tumor of the urinary system that seriously threatens human life and health. This study aims to explore the role of the traditional Chinese medicine Leptochloa chinensis in the pathogenesis of RCC. Meanwhile, this study also revealed the molecular biological mechanism of its antitumor activity. Methods. Human RCC 786-O cells were cultured in the RPMI-1640 medium, which contains different concentrations of Leptochloa chinensis (1,000, 3,000, and 9,000 μg/ml). MTT and flow cytometry assays were used to detect the viability of 786-O cells. Transwell and wound healing assays were used to detect cell metastasis. The protein expression was observed by western blot analysis. Results. Leptochloa can inhibit cell proliferation and induce apoptosis in RCC 786-O cells. In addition, Leptochloa can weaken the migration and invasion of 786-O cells. More importantly, Leptochloa can block the mTOR pathway by inhibiting the protein expression of p-mTOR. Moreover, the high concentration of Leptochloa chinensis has a better inhibitory effect on 786-O cells. Conclusion. The traditional Chinese medicine Leptochloa chinensis inhibits the viability and metastasis of 786-O cells by blocking the mTOR pathway.

Effects of Angelica Fritillaria Kushen Pill on Renal Function and Immune Function after Laparoscopic Radical Nephrectomy for Patients with Renal Carcinoma

“…Modern researches have shown [20] that Chinese medicine-assisted RCC postoperative treatment has a relatively clear role in improving patients' clinical symptoms, protecting renal function, regulating body immunity, preventing tumor recurrence and metastasis, and improving the quality of life of patients. Tan et al [21] found that the incidence of side effects in places such as cardiovascular system and digestive system in patients treated with traditional Chinese medicine was significantly lower than that of the single western medicine treatment when patients were treated after radical resection of RCC with TCM on the basis of basic western medicine treatment. Zhong et al [22] found that combined Chinese medicine therapy was more stable for patients with renal cancer after laparoscopic radical resection and prolonged survival time.…”

Effect of Human-Oriented Fine Nursing on Psychological Emotion, Life Quality, and Nursing Satisfaction of Patients Undergoing Laparoscopic Radical Nephrectomy

“…Based on the holistic view and syndrome differentiation, traditional Chinese medicine believes that renal cell cancer is caused by deficiency of kidney qi and aggregation of pathogenic toxins [ 4 ]. The application of TCM treatment can improve the healthy qi, stabilize the cancer focus, prevent recurrence and metastasis, and thus achieve the purpose of improving the quality of life of patients and prolonging their survival [ 5 , 6 ].…”

“…e application of TCM treatment can improve the healthy qi, stabilize the cancer focus, prevent recurrence and metastasis, and thus achieve the purpose of improving the quality of life of patients and prolonging their survival [5,6].…”

Synergistic anti-tumour activity of sorafenib in combination with pegylated resveratrol is mediated by Akt/mTOR/p70S6k-4EBP-1 and c-Raf7MEK/ERK signaling pathways

“…TCM combined with chemotherapeutic drugs can act on multiple pathways at the same time, enhance the anti-tumor efficacy of chemotherapeutic drugs, reduce the adverse reactions of chemotherapeutic drugs and also avoid the emergence of drug resistance [ 8 ]. So the TCM combined with targeted drugs, such as sorafenib, has a broad application prospect in the treatment of cancer [ 12 ]. Resveratrol is a natural polyphenolic antioxidant, which is widely present in grapes, peanuts and a variety of TCMs [ 13 , 14 ].…”