Chinese Oak Tasar Silkworm <i>Antheraea pernyi</i> Silk Proteins: Current Strategies and Future Perspectives for Biomedical Applications

Silva, Simone S.; Kundu, Banani; Lu, Shenzhou; Reis, Rui L.; Kundu, Subhas C.

doi:10.1002/mabi.201800252

Cited by 38 publications

(19 citation statements)

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“…However, the amino acid composition SP of A. proylei is different from those reported earlier in mulberry and non-mulberry silkworms [ Table 1]. This corroborates with earlier studies of sericin from different species of silkworms [5,16] The present study aims to evaluate the anti-cancer potential of SP on three types of cancers namely lung cancer, cervical cancer, and prostate cancer using A549, HeLa, and PC3 cell lines. Though such properties were observed in sericin of B. mori, it is the rst report on SP from the cocoon of A. proylei.…”

Section: Discussionsupporting

confidence: 85%

“…Depending upon the species from where the sericin is obtained, the amino acid composition varies considerably. Sericin from wild silkworms has a higher content of threonine, glutamic acid, cysteine and phenylalanine and a lower content of serine, proline, methionine, glucosamine, galactosamine, and histidine (5). Owing to the difference in the proportions of amino acid compositions in the domesticated silkworm, Bombyx .mori and the wild type silkworm, Antheraea sp., there may be variation in the bioactivity.…”

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confidence: 99%

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Sericin Preparation from Cocoons of Oak Tasar Silkworm Antheraea proylei J. Induces Apoptosis in a Caspase-dependent Manner in A549 and HeLa Cells and Caspase-independent Manner in PC3 Cells

Devi

Singh

et al. 2020

Preprint

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BackgroundDespite much progress in understanding the biology of cancer disease, advancement in technology for early diagnosis, the expanding array of anticancer drugs, and treatment modalities, the global cancer burden is still significant and increasing. It is estimated that the new cases of cancer in the year 2040 will be 29.4 million per year globally. Sericin, an adhesive protein of silk cocoon, is a potential protein in various biomedical applications including cancer therapeutics. The present study evaluates the anticancer property of sericin prepared from cocoons of Antheraea proylei J. (A. proylei ) against human lung cancer (A549), cervical cancer (HeLa), and prostate cancer (PC3) cell lines. This is the first report of the anti-cancer activity of the non-mulberry silkworm A. proylei. MethodsSericin preparation (SP) was prepared from cocoons of A. proylei J. by the process of the degumming method. The amino acid composition of the SP was determined by HPLC. Cytotoxicity activity was assessed by MTT assay and genotoxicity activity was assessed by comet assay. Cleavage of caspase and PARP proteins and phosphorylation of MAPK pathway members were analyzed by Western blotting. Cell cycle analysis was done by FACS flow cytometry.Results SP causes cytotoxicity to A549, HeLa, and PC3 cell lines with the IC50 values ranging from 3.4-3.9 µg/µl. SP induces apoptosis in a dose-dependent manner through caspase-3 and p38/SAPK/ERK pathways in A549 and HeLa cells whereas in PC3 cells SP induces apoptosis independent of caspase but through p38 pathway. Moreover, in the case of A549 and HeLa cells, SP induces cell cycle arrest at the S phase whereas at the G0 phase in the case of PC3 cells in a dose-dependent manner.ConclusionThe difference in the molecular mechanisms of apoptosis induced by SP in A549 and HeLa cell lines, and in PC3 cell lines may be due to the difference in the genotypes of the cancer cell lines where A549 and HeLa cells are being non-malignant and p53 positive whereas PC3 cell is being malignant and p53 negative. The overall results of the present study envisage the possibility of using SP as an anti-tumorogenic agent.

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Section: Discussionsupporting

confidence: 85%

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confidence: 99%

Sericin Preparation from Cocoons of Oak Tasar Silkworm Antheraea proylei J. Induces Apoptosis in a Caspase-dependent Manner in A549 and HeLa Cells and Caspase-independent Manner in PC3 Cells

Devi

Singh

et al. 2020

Preprint

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“…A. pernyi is the second most sericulturally‐relevant lepidopteran species after the domesticated silkworm B. mori . In addition to its value in the production of silk, food, and biomedicine (Li et al., 2017; Liu, You, et al., 2014; Silva et al., 2019), its diverse, distinct, and striking body colours that develop during the larval stage attract many tourists to its local regions. Thus, development of A. pernyi sericulture can help mountainous farmers economically.…”

Section: Discussionmentioning

confidence: 99%

A chromosome‐scale genome assembly of Antheraea pernyi (Saturniidae, Lepidoptera)

Duan

Liu

et al. 2020

Molecular Ecology Resources

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Antheraea pernyi is a semi‐domesticated lepidopteran insect species valuable to the silk industry, human health, and ecological tourism. Owing to its economic influence and developmental properties, it serves as an ideal model for investigating divergence of the Bombycoidea super family. However, studies on the karyotype evolution and functional genomics of A. pernyi are limited by scarce genomic resource. Here, we applied PacBio sequencing and chromosome structure capture technique to assemble the first high‐quality A. pernyi genome from a single male individual. The genome is 720.67 Mb long with 49 chromosomes and a 13.77‐Mb scaffold N50. Approximately 441.75 Mb, accounting for 60.74% of the genome, was identified as repeats. The genome comprises 21,431 protein‐coding genes, 85.22% of which were functionally annotated. Comparative genomics analysis suggested that A. pernyi diverged from its common ancestor with A. yamamai ~30.3 million years ago, and that chromosome fission contributed to the increased chromosome number. The genome assembled in this work will not only facilitate future research on A. pernyi and related species but also help to progress comparative genomics analyses in Lepidoptera.

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“…SF, a natural polymer protein extracted from silk, exhibits good biocompatibility, stable mechanical properties, tunable biodegradation, and good preservation of biomolecule activity . Its conformation can be divided into Silk I (random coil and α ‐helix) and Silk II (β‐sheet) ,.…”

Section: Structure and Properties Of Sfmentioning

confidence: 99%

Combined Application of Graphene‐Family Materials and Silk Fibroin in Biomedicine

et al. 2019

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The main graphene‐family materials used in biomedicine include graphene, graphene oxide, and reduced graphene oxide. They have been well documented for their distinctive microstructure and excellent physicochemical properties. Although they also have some shortcomings, such as slow biodegradation and dose‐dependent biotoxicity, these problems can be solved by using them in conjunction with other biomaterials. Silk fibroin (SF), a natural polymer material with many advantageous properties, has been used widely in biomedicine. However, SF requires modification by other biomaterials to improve its mechanical properties and control its degradation rate for further use. In recent years, the combined application of graphene‐family materials and SF has increased gradually, and the effects of it on cell behavior have been preliminary investigated, such as promoting cell adhesion, proliferation, and differentiation. Further studies of the combined use have been conducted in biomedicine, including tissue engineering, biosensor, and other biomedical usage. In this paper, this combined application has been outlined and summarized, and some envisioned challenges and future perspectives have been mentioned. We hope that this review will provide some reference and inspiration for the combined application of graphene‐family materials and SF in biomedicine in the future.

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Chinese Oak Tasar Silkworm Antheraea pernyi Silk Proteins: Current Strategies and Future Perspectives for Biomedical Applications

Cited by 38 publications

References 73 publications

Sericin Preparation from Cocoons of Oak Tasar Silkworm Antheraea proylei J. Induces Apoptosis in a Caspase-dependent Manner in A549 and HeLa Cells and Caspase-independent Manner in PC3 Cells

Sericin Preparation from Cocoons of Oak Tasar Silkworm Antheraea proylei J. Induces Apoptosis in a Caspase-dependent Manner in A549 and HeLa Cells and Caspase-independent Manner in PC3 Cells

A chromosome‐scale genome assembly of Antheraea pernyi (Saturniidae, Lepidoptera)

Combined Application of Graphene‐Family Materials and Silk Fibroin in Biomedicine

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