Ching-fang-pai-tu-san inhibits the release of influenza virus

Hsieh, Chung-Fan; Yen, Hung-Rong; Liu, Chih-Hao; Lin, Shiming; Horng, Jim-Tong

doi:10.1016/j.jep.2012.09.035

Cited by 9 publications

(17 citation statements)

References 47 publications

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“…Based on these results, we suggest that TDSWex can inhibit NA activity. To confirm whether the antiviral effect of TDSWex correlated with its effect on NA function, we performed a plaque reduction assay1630. The plaque numbers were significantly reduced by about 36% and 32%, respectively, when influenza viruses were treated with 0.1 mg/mL or 0.05 mg/mL of TDSWex during viral replication (#1 vs. #2 and #1 vs. #3, Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Total RNA extraction and RT-qPCR were performed as previously described30. To quantify changes in gene expression, the ΔCt method was used to calculate the relative changes normalized against GAPDH.…”

Section: Methodsmentioning

confidence: 99%

“…The cells were treated with or without addition of TDSWex (0.1 mg/mL) in E0 and harvested at the indicated time points for western immunoblotting as previously described30. The antibodies against virus M1 antibody (catalogue #1321) were from ViroStat (Portland, ME).…”

Section: Methodsmentioning

confidence: 99%

“…TDSWex (0.1 mg/mL) in E0 was added at –3 to –1 h (preadsorption), –1 to 0 h (adsorption), 0 to 12 h, and –3 to 12 h. All the supernatants were collected at 12 h p.i. for titer determination by plaque assay30.…”

Section: Methodsmentioning

confidence: 99%

“…This assay was previously described with minor modification3057. Briefly, MDCK cells were incubated on ice for 20 min before infected with 3 × TCID 50 of WSN in the presence of different concentrations of TDSWex on ice for 1 h. The medium containing the nonadsorbed virus was removed and the cells were then washed twice with HBSS.…”

Section: Methodsmentioning

confidence: 99%

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An extract from Taxodium distichum targets hemagglutinin- and neuraminidase-related activities of influenza virus in vitro

Hsieh

Chen

Lin

et al. 2016

Sci Rep

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Influenza virus remains an emerging virus and causes pandemics with high levels of fatality. After screening different plant extracts with potential anti-influenza activity, a water extract of Taxodium distichum stems (TDSWex) showed excellent activity against influenza viruses. The EC50 of TDSWex was 0.051 ± 0.024 mg/mL against influenza virus A/WSN/33. TDSWex had excellent antiviral efficacy against various strains of human influenza A and B viruses, particularly oseltamivir-resistant clinical isolates and a swine-origin influenza strain. We observed that the synthesis of viral RNA and protein were inhibited in the presence of TDSWex. The results of the time-of-addition assay suggested that TDSWex inhibited viral entry and budding. In the hemagglutination inhibition assay, TDSWex inhibited the hemagglutination of red blood cells, implying that the extract targeted hemagglutin-related functions such as viral entry. In the attachment and penetration assay, TDSWex showed antiviral activity with EC50s of 0.045 ± 0.026 and 0.012 ± 0.003 mg/mL, respectively. In addition, TDSWex blocked neuraminidase activity. We conclude that TDSWex has bimodal activities against both hemagglutinin and neuraminidase during viral replication.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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