Chip-calorimetric monitoring of biofilm eradication with antibiotics provides mechanistic information

Mariana, F.; Buchholz, Friederike; Lerchner, J.; Neu, Thomas R.; Harms, Hauke; Maskow, Thomas

doi:10.1016/j.ijmm.2012.12.009

Cited by 26 publications

(12 citation statements)

References 44 publications

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“…As an alternative, chip calorimeters [ 31 – 34 ] used in combination with flow-through systems can allow a much higher throughput than conventional isothermal microcalorimeters. Such chip calorimeters have recently been used as bio-activity monitors in exploratory studies [ 35 , 36 ]. However, their small volume (10 nL to 100 μL) and their lower sensitivity compared to conventional instruments, limit their use with dilute cell suspensions and heterogeneous materials like tissue preparations and small animals.…”

Section: Introductionmentioning

confidence: 99%

Isothermal microcalorimetry accurately detects bacteria, tumorous microtissues, and parasitic worms in a label‐free well‐plate assay

Braissant

Keiser

Meister

et al. 2015

Biotechnology Journal

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Isothermal microcalorimetry is a label-free assay that allows monitoring of enzymatic and metabolic activities. The technique has strengths, but most instruments have a low throughput, which has limited their use for bioassays. Here, an isothermal microcalorimeter, equipped with a vessel holder similar to a 48-well plate, was used. The increased throughput of this microcalorimeter makes it valuable for biomedical and pharmaceutical applications. Our results show that the sensitivity of the instrument allows the detection of 3 × 104 bacteria per vial. Growth of P. mirabilis in Luria Broth medium was detected between 2 and 9 h with decreasing inoculum. The culture released 2.1J with a maximum thermal power of 76 μW. The growth rate calculated using calorimetric and spectrophotometric data were 0.60 and 0.57 h–1, respectively. Additional insight on protease activities of P. mirabilis matching the last peak in heat production could be gathered as well. Growth of tumor microtissues releasing a maximum thermal power of 2.1 μW was also monitored and corresponds to a diameter increase of the microtissues from ca. 100 to 428 μm. This opens new research avenues in cancer research, diagnostics, and development of new antitumor drugs. For parasitic worms, the technique allows assessment of parasite survival using motor and metabolic activities even with a single worm.

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Section: Introductionmentioning

confidence: 99%

Isothermal microcalorimetry accurately detects bacteria, tumorous microtissues, and parasitic worms in a label‐free well‐plate assay

Braissant

Keiser

Meister

et al. 2015

Biotechnology Journal

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show abstract

“…Lerchner et al (2008) discuss a flow calorimeter based on a solid-state (or chip) design and use it to study Pseudomonas putida biofilms, although in this case the biofilms were cultured prior to calorimetric measurement. Mariana et al (2013) used the same chip calorimeter to monitor eradication of P. putida biofilms with antibiotics. Morais et al (2014) have also used chip calorimeters to investigate the antimicrobial efficacy of biofilms.…”

Section: Measurement Of Biofilmsmentioning

confidence: 99%

◾ Calorimetric Approaches to Studying Complex Protein Structure–Function–Stability Relationships in Conformational Diseases: The Case of Cystathionine β-Synthase

2016

Biocalorimetry

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“…Lerchner et al [34] discuss a flow calorimeter based on a solid-state (or chip) design and use it to study Pseudomonas putida biofilms, although in this case the biofilms were cultured prior to calorimetric measurement. Mariana et al [35] used the same chip calorimeter to monitor eradication of P. putida biofilms with antibiotics. Morais et al [36] have also used chip calorimeters to investigate antimicrobial efficacy of biofilms.…”

Section: Flow Calorimetry For Biofilm Studiesmentioning

confidence: 99%

Development of a flow system for studying biofilm formation on medical devices with microcalorimetry

Said

Walker

Parsons

et al. 2015

Methods

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Isothermal microcalorimetry (IMC) is particularly suited to the study of microbiological samples in complex or heterogeneous environments because it does not require optical clarity of the sample and can detect metabolic activity from as few as 10 4 CFU/mL cells.While the use of IMC for studying planktonic cultures is well established, in the clinical environment bacteria are most likely to be present as biofilms. Biofilm prevention and eradication present a number of challenges to designers and users of medical devices and implants, since bacteria in biofilm colonies are usually more resistant to antimicrobial agents.Analytical tools that facilitate investigation of biofilm formation are therefore extremely useful.While it is possible to study pre-prepared biofilms in closed ampoules, better correlation with in-vivo behaviour can be achieved using a system in which the bacterial suspension is flowing. Here, we discuss the potential of flow calorimetry for studying biofilms and report the development of a simple flow system that can be housed in a microcalorimeter. The use of the flow system is demonstrated with biofilms of Staphylococcus aureus.

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Chip-calorimetric monitoring of biofilm eradication with antibiotics provides mechanistic information

Cited by 26 publications

References 44 publications

Isothermal microcalorimetry accurately detects bacteria, tumorous microtissues, and parasitic worms in a label‐free well‐plate assay

Isothermal microcalorimetry accurately detects bacteria, tumorous microtissues, and parasitic worms in a label‐free well‐plate assay

◾ Calorimetric Approaches to Studying Complex Protein Structure–Function–Stability Relationships in Conformational Diseases: The Case of Cystathionine β-Synthase

Development of a flow system for studying biofilm formation on medical devices with microcalorimetry

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