Chiral analysis of aliphatic short chain organic acids by capillary electrophoresis

Abstract

Short chain organic acids play an important role in different areas such as biochemistry, clinical chemistry, or the food industry. The enantiomeric ratio of chiral metabolites is an important parameter for the understanding of metabolic processes and in many cases it can serve diagnostic purposes. On the other hand, the presence of racemates in food products could indicate the use of organic acids as additives; this is not always permitted and needs to be controlled. The short chain of these acids makes difficult the three point interaction generally accepted as necessary for chiral recognition. Relatively recent publications have demonstrated the feasibility of their direct chiral separation in capillary electrophoresis by various techniques utilizing exchange capillary electrophoresis, macrocyclic antibiotics, cyclodextrins, ion‐pair method, and transition metal complexes. The present article describes existing methods and strategies proposed to advance these areas.

Recent advances in the application of capillary electromigration methods for food analysis

“…Several reviews have been published on the application of CE methods in food analysis in the last three years, which is a good demonstration of the possibilities of this technique in this particular field. Table 1 summarizes the different reviews published on this topic [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19], including the aforementioned review by Frazier and Papadopoulou [1].…”

Capillary electrophoresis for short‐chain organic acids and inorganic anions in different samples

“…The method, which does not require any other sample pretreatment more than dilution and filtration, was applied to the identification of both isomers in body fluids as plasma, urine, amniotic fluid, and CSF [113]. This very complex area exceeds the intention of the present paper, but to mention that a comprehensive review on the subject has been recently published [142].…”

Chiral separation of disease biomarkers with 2‐hydroxycarboxylic acid structure

“…Different studies have approached the enantioseparation of 2-hydroxycarboxylic acids. Some of them are ligand exchange CE [19][20][21], ligand exchange LC [22], GC analysis after derivatization [23], LC after derivatization [24], direct LC by using macrocyclic antibiotic chiral stationary phases (CSPs) [25][26][27], and quinine-or quinidine-derived CSPs [28][29][30][31][32][33], amongst others.…”