Chiral Analysis of Biogenic D,L-Amino Acids Derivatized by N-Fluorenylmethoxycarbonyl-L-alanyl N-Carboxyanhydride Using High-Performance Liquid Chromatography

Zbrožek, J.; Pumera, Martin; Flegel, Martin

doi:10.1093/chromsci/40.9.505

Cited by 6 publications

(7 citation statements)

References 13 publications

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“…Because of its excellent sensitivity, indirect HPLC utilizing chiral derivatization reagent with the creation of two diastereomers is often used in bioanalysis, in contrast to industry. [54][55][56] However, this indirect approach necessitates the presence of a functional group in the analyte. When a chiral deriving reagent is given to the sample, it reacts with these functions to produce well-resolved diastereomers using a reversed-phase column (C18 or C8).…”

Section: Chiral Bioanalytical Methodsmentioning

confidence: 99%

“…The most extensively utilized of the four procedures is HPLC. Because of its excellent sensitivity, indirect HPLC utilizing chiral derivatization reagent with the creation of two diastereomers is often used in bioanalysis, in contrast to industry 54–56 . However, this indirect approach necessitates the presence of a functional group in the analyte.…”

Section: Chiral Bioanalytical Methodsmentioning

confidence: 99%

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Advances and challenges in the pharmacokinetics and bioanalysis of chiral drugs

2022

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Enantioselective analytical approaches are essential for monitoring pharmacokinetics and acquiring accurate data to better understand the role of stereochemistry in pharmacokinetics. Enantioselectivity significantly impacts the pharmacokinetics of chiral drugs, especially in metabolic profile, leading to toxicity of enantiomer. Consequently, there is a need to study the pharmacokinetics of enantiomerically pure drugs and racemates as they differ in affinity with enzymes and proteins. Combining the best enantioseparation conditions with the specified biological matrix and the intended purpose of the analysis is a challenging task. This review discusses the importance of chirality in stereoselective pharmacokinetics with more relevant examples, various enantioselective analytical techniques, and stationary phases employed. Challenges such as lack of universal chiral columns, biological inversion of the isomers, and others have been discussed. Further presented the recent advances in the screening of chiral drugs and innovative improvements in the analytical approaches for chiral molecule analysis such as supercritical fluid chromatography, simulated moving bed chromatography, and other techniques are discussed.

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Section: Chiral Bioanalytical Methodsmentioning

confidence: 99%

Section: Chiral Bioanalytical Methodsmentioning

confidence: 99%

Advances and challenges in the pharmacokinetics and bioanalysis of chiral drugs

2022

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“…The resolution of racemic mixtures of optical isomers of amino acids is an important issue in pharmaceutical and biotechnology fields because pharmaceutical or bioactive effects of enantiomers are often different [41]. Equally important is to distinguish between amino acid enantiomers in the case of space exploration, since the homochirality of amino acids offers a way of distinguishing between abiotic and biotic origins [42][43][44].…”

Section: Chiral Separationsmentioning

confidence: 99%

Microfluidics in amino acid analysis

Pumera

2007

Electrophoresis

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Microfluidic devices have been widely used to derivatize, separate, and detect amino acids employing many different strategies. Virtually zero-dead volume interconnections and fast mass transfer in small volume microchannels enable dramatic increases in on-chip derivatization reaction speed, while only minute amounts of sample and reagent are needed. Due to short channel path, fast subsecond separations can be carried out. With sophisticated miniaturized detectors, the whole analytical process can be integrated on one platform. This article reviews developments of lab-on-chip technology in amino acid analysis, it shows important design features such as sample preconcentration, precolumn and postcolumn amino acid derivatization, and unlabeled and labeled amino acid detection with focus on advanced designs. The review also describes important biomedical and space exploration applications of amino acid analysis on microfluidic devices.

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“…The flow rate was 1.25 mL/min, and amino acid derivatives were detected at 260 nm. It was established that the method can be used for the enantiomeric separation of Phe, Tyr, Met, Arg, Val, Leu, Cys, Ile, Trp and Asn (Zbrozek et al, 2002).…”

Section: Chiral Separations By Hplcmentioning

confidence: 99%

Chromatography of amino acids and short peptides. New advances

Cserháti

2007

Biomedical Chromatography

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The newest results in the application of various chromatographic methods (gas-liquid chromatography, liquid chromatographic techniques, electrically driven systems) for the separation and quantitative determination of amino acids and short peptides in pure state and in complicated matrices are compiled. The results are concisely described and critically evaluated. The future trends of the chromatographic analysis of amino acids and short peptides are briefly discussed.

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Chiral Analysis of Biogenic D,L-Amino Acids Derivatized by N-Fluorenylmethoxycarbonyl-L-alanyl N-Carboxyanhydride Using High-Performance Liquid Chromatography

Cited by 6 publications

References 13 publications

Advances and challenges in the pharmacokinetics and bioanalysis of chiral drugs

Advances and challenges in the pharmacokinetics and bioanalysis of chiral drugs

Microfluidics in amino acid analysis

Chromatography of amino acids and short peptides. New advances

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