Chiral determination of a novel acaricide cyflumetofen enantiomers in cucumber, tomato, and apple by HPLC

Abstract

A simple chiral analytical method was developed for the enantiomeric determination of cyflumetofen in cucumber, tomato, and apple by normal-phase HPLC. The effects of mobile phase composition and column temperature on the enantioseparation were evaluated. Excellent separation was achieved at 25°C on a Chiralpak AD-H column, with a mixture of n-hexane and 2-propanol (95:5, v/v) as mobile phase at a flow rate of 1.0 mL/min detecting at 234 nm. The resolution of cyflumetofen enantiomers was up to 5.5. The elution order of the enantiomers was determined by an online OR-2090 detector, which was performed under the same chromatographic conditions. The first eluted enantiomer was (-)-cyflumetofen and the second eluted one was (+)-cyflumetofen. The method was validated for linearity, repeatability, accuracy, LOD, and LOQ. LOD ranged from 0.1 to 0.15 mg/kg, with the LOD varying from 0.33 to 0.5 mg/kg for each enantiomer, respectively. The average recoveries of the pesticide ranged from 71.4 to 102.0% at all fortification levels. The precision values associated with the analytical method, expressed as RSD values, were below 14.8% in all matrices. The method was then successfully applied to detect cyflumetofen enantiomers in real samples.

Enantioselective separation and pharmacokinetic dissipation of cyflumetofen in field soil by ultra‐performance convergence chromatography with tandem mass spectrometry

“…The separation factor (α) decreased as the proportion of 2‐propanol increased, likely due to the high viscosity of 2‐propanol. The results are similar to those reported by Chen . CO 2 /isopropanol (96.5:3.5, v/v) was chosen as the mobile phase due to the good resolution (3.06) and short retention time (3.11, 3.63 min) obtained using these compounds.…”

“…The other columns were insufficiently able to discriminate between the cyflumetofen stereoisomers. Previous research also found that cyflumetofen is well separated on a coated‐type amylose stationary phase column using HPLC .…”

“…Previous research reported the enantiomeric determination of cyflumetofen in cucumber, tomato, and apple by normal‐phase HPLC . However, this method has several disadvantages including high solvent consumption and lengthy retention times.…”

Distribution behaviour of acaricide cyflumetofen in tomato during home canning

“…However, a Joint FAO/WHO Meeting found that cyflumetofen is potentially carcinogenic in male rats (FAO/WHO 2006). Some researchers have reported analytical methods for cyflumetofen (Chen et al 2013;Li et al 2013) and toxicity assessments (Takahashi et al 2012), but few studies have focused on the concentration change during food processing. There are no MRLs for the related processed commodities.…”

Preparation of cyflumetofen nanocapsules and study on the controlled release performance and its field efficacy

“…Cyflumetofen is a novel type of benzoyl acetonitrile acaricide developed by Ostuka AgriTechno Co., Ltd (Tokyo, Japan) . Cyflumetofen has a significant controlling effect on mites on fruits, vegetables, flowers and other plants without cross‐resistance to existing acaricides, and is very safe to non‐target plants, mammals and aquatic organisms, both beneficial organisms and natural enemies . At present, the main cyflumetofen formulation on the market is a 20% cyflumetofen suspension, which is degraded to its de‐esterified form (AB‐1) for killing mites after the active ingredient has entered into them.…”