Chiral discrimination using an immunosensor

Abstract

Based on the stereoselectivity of immunoglobulins, we have developed a new chiral sensor for the detection of low-molecular-weight analytes. Using surface plasmon resonance detection, enantiomers of free, underivatized alpha-amino acids can be monitored in a competitive assay by their interaction with antibodies specific for the chiral center of this class of substances. The sensitivity to the minor enantiomer in nonracemic mixtures exceeds currently available methods; therefore, such immunosensors can readily detect traces of enantiomeric impurities and are attractive for a range of applications in science and industry.

Contributions to the theory and practice of the chromatographic separation of enantiomers

“…Thus, Heike Hofstetter (née Diebold) investigated the first use of enantioselective combinatorial selectors (cyclohexapeptide libraries) in capillary electrophoresis together with the group of Günther Jung, Tübingen, Germany, 113 while Oliver Hofstetter used antibodies raised against D -and L -amino acids in an enantioselective biosensor system 114 and for the determination of trace amounts of enantiomeric impurities (D -in L -amino acids) 115 in a cooperation with Meir Wilchek and Bernard S. Green, Rehovot, Israel. Birgit Gross separated an inherently chiral [60]fullerene derivative on Gil-Av's TAPA stationary phase by enantioselective HPLC together with Andreas Hirsch, Erlangen, Germany.…”

Chiral Biosensors and Immunosensors

“…Chiral discrimination was shown for the enantiomers of tyrosine, DOPA (3,4-dihydroxyphenylalanine), norleucine (Fig 6.) and tryptophan. The immunosensor allowed to detect 0.01% of the minor enantiomer present in the major enantiomer sample (Hofstetter et al, 1999). Also a magnetic relaxation switching appears can be applied as a detection method for the construction of chiral immunosensors.…”

Chiral Nanoscience and Nanotechnology

“…Based on the stereoselectivity of immunoglobulins, a chiral sensor for the detection of low-molecular-weight analytes has been reported 41 . Such immuno-sensors can readily detect traces of enantiomeric impurities and are attractive for a range of applications in science and industry.…”