Chiral separation and determination of amino acids in real samples by LE-MEKC using Cu(II)-l-proline as chiral selector

Wu, Yongjiang; Zhai, Yu-Yao; Zhang, Hongfen; Jing, Huanwang; Chen, Anjia

doi:10.1016/j.jchromb.2014.07.001

Cited by 20 publications

(7 citation statements)

References 21 publications

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“…Therefore, it is important to investigate the effect of the concentration ratio of Cu (II) to L-Ile on resolution. Figure 3A shows the results of resolutions upon changing the concentration ratio of Cu (II) to L-Ile, which was achieved by altering the concentration of L-Ile (4,8,12,16,20, and 24 mmol/L) with a fixed concentration of Cu (II) of 4 mol/L constant in the running buffer (10 mmol/L NH 4 OAc containing 5% methanol, 20 mmol/L SDS, pH 8.5 or 9.0). As can be seen in Figure 3A, the optimal resolution was obtained when the ratio of Cu(II) to L-Ile was 1:5.…”

Section: Effect Of the Ratio Of Cu (Ii) To L-ile And Concentration mentioning

confidence: 99%

Enantioseparation of ofloxacin and its four related substances with ligand exchange–micellar electrokinetic chromatography using copper(II)‐L‐isoleucine complex as chiral selector

Liu

Wang

2017

Chirality

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A ligand-exchange micellar electrokinetic capillary electrophoresis system with copper(II)-L-isoleucine complexes as the chiral selector incorporated in micelles of sodium dodecyl sulfate (SDS) was developed for the enantioseparation of ofloxacin and its four related substances (impurities A, C, E, and F). The effects of important parameters affecting separation such as buffer pH, SDS concentration, chiral selector concentration, and organic additive were investigated in detail. Under optimum experimental conditions, enantioseparation of ofloxacin, impurities A, C, E, and F enantiomers was accomplished with resolutions of 4.28, 2.83, 3.40, 3.58, and 2.46, respectively. Further, simultaneous separation of impurities A, C, E, and F enantiomers was achieved using 10 mmol/L NH OAc as the running buffer containing 4 mmol/L copper sulfate,20 mmol/L L-isoleucine, 20 mmol/L SDS, and 5% methanol at pH 8.5. To the best of our knowledge, the simultaneous enantioseparation of four impurities of ofloxacin has not been reported previously.

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Section: Effect Of the Ratio Of Cu (Ii) To L-ile And Concentration mentioning

confidence: 99%

Enantioseparation of ofloxacin and its four related substances with ligand exchange–micellar electrokinetic chromatography using copper(II)‐L‐isoleucine complex as chiral selector

Liu

Wang

2017

Chirality

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“…The selectivity for all the AAs changed with increase in the concentration of different surfactants. Sodium dodecyl sulfate was found to improve the selectivity between analytes for enantioseparations of dansyl AAs by ligand-exchange capillary electrophoresis [80] and of three underivatized AAs by ligand-exchange micellar electrokinetic chromatography (LE-MEKC) [81].…”

Section: Selectivity and Retention In Lecmentioning

confidence: 99%

“…Cu(II) complex of l-proline as chiral additive provided good selectivity and high resolutions for enantioseparation and determination of three underivatized AAs in human urine, AAs injection, and AA oral liquid [81].…”

Section: Selectivity and Retention In Lecmentioning

confidence: 99%

Enantioresolution of Amino Acids: A Decade’s Perspective, Prospects and Challenges

Tanwar

Bhushan

2015

Chromatographia

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“…Obtaining high purity optical isomers from racemates has always been very challenging. At present, many separation methods are carried out to get enantiopure isomers, such as TLC , HPLC , CE , SFC , ligand‐exchange MEKC , GC , magnetic assisted sorption separation , LLE techniques , etc. Although many chiral resolution methods have been developed for MA and its derivatives , endeavor on scale‐up preparation and simultaneous chiral recognition/separation are still needed.…”

Section: Introductionmentioning

confidence: 99%

Facile enantioseparation and recognition of mandelic acid and its derivatives in self‐assembly interaction with chiral ionic liquids

Cai

Gao

Yin

et al. 2019

J of Separation Science

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Mandelic acid and its derivatives are important medical intermediates in the pharmaceutical industry. Different stereoisomers exhibited distinct biological properties to human bodies. Given that, enantioselective recognition and separation of mandelic acid are of great importance. In this study, four novel different types of chiral ionic liquids bearing designed functional groups were synthesized and successful enantioselective precipitation with mandelic acid and its derivatives. That is, (R, R)-chiral ionic liquid 1 can coprecipitated with S-mandelic acid and its derivatives was observed. In addition, good correlation coefficient is achieved by using electrospray mass spectrum at negative ion pattern for quick analysis of the enantioselective precipitation, which could be served as a method of enantioselective recognition. The possible intermolecular interactions are established after systematical studies by NMR spectroscopy and DFT calculations. K E Y W O R D S chiral separation, coprecipitation, ionic liquids, mandelic acid J Sep Sci 2019;42:3589-3598.

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Chiral separation and determination of amino acids in real samples by LE-MEKC using Cu(II)-l-proline as chiral selector

Cited by 20 publications

References 21 publications

Enantioseparation of ofloxacin and its four related substances with ligand exchange–micellar electrokinetic chromatography using copper(II)‐L‐isoleucine complex as chiral selector

Enantioseparation of ofloxacin and its four related substances with ligand exchange–micellar electrokinetic chromatography using copper(II)‐L‐isoleucine complex as chiral selector

Enantioresolution of Amino Acids: A Decade’s Perspective, Prospects and Challenges

Facile enantioseparation and recognition of mandelic acid and its derivatives in self‐assembly interaction with chiral ionic liquids

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