Chiral separation of dansyl-amino acids in a nonaqueous medium by capillary electrophoresis

Valkó, István E.; Sirén, Heli M. M.; Riekkola, Marja‐Liisa

doi:10.1016/0021-9673(96)00003-9

Cited by 85 publications

(30 citation statements)

References 53 publications

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“…Enantioseparation in pure organic solvents was first reported by Ye and Khaledi in 1994 [150], and recent advances in the use of nonaqueous CE as well as the effect of organic solvents on separation performances involving optical isomers were reviewed by Wang and Khaledi [151], as well as by Riekkola and co-workers [5]. For example, Valkó et al [152] reported that the problem associated with the low solubility of b-CD in water can be resolved by dissolving this commonly employed chiral selector in N-methylformamide (NMF). b-CD was about 40 times more soluble in NMF than in water and, importantly, NMF was found to have a marked effect on the stability of the analyte (dansyl amino acids)-chiral selector complex.…”

Section: Optical Isomersmentioning

confidence: 99%

Effects of organic solvents on sample pretreatment and separation performances in capillary electrophoresis

Huie

2003

Electrophoresis

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A review of recent developments in theoretical as well as application studies concerning the use of organic solvents, either as purely nonaqueous solvents, hydro-organic mixtures, or a combination of an organic solvent with another organic modifier(s), in the sample matrix and/or separation buffer for effecting sample pretreatment and/or improving separation performances in capillary electrophoresis (CE) is presented. In particular, recent advances made in furthering the basic understanding of selectivity changes that occur in capillary zone electrophoresis due the presence of organic solvents in the separation medium, based on in-depth studies of fundamental processes, such as acid-base chemistry, ion-ion and ion-solvent interactions, were discussed in detail. The utilization of organic solvents for improving the resolution of highly challenging and important separations, i.e., those involving the separation of positional and optical isomers, was also critically reviewed. Furthermore, a comprehensive survey of the use of organic solvents for on-line sample pretreatment, e.g., minimizing aggregation and maximizing solubilization of hydrophobic analytes, improving concentration detection sensitivity for analytes via the use of sample stacking, was presented and discussed. Moreover, recent applications involving the use of organic solvents for improving the CE separations of a variety of molecular species with significance in various disciplines, including biological, environmental and pharmaceutical areas, were summarized and tabulated.

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Section: Optical Isomersmentioning

confidence: 99%

Effects of organic solvents on sample pretreatment and separation performances in capillary electrophoresis

Huie

2003

Electrophoresis

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“…In this section, three neutral CDs (a-CD, b-CD, and g-CD) were tested together with SC to be used as the chiral selectors for prothiofos, sulprofos, and profenofos. Though amide-type solvents are preferred to these neutral selectors [20,21,[31][32][33], they could not be used as the solvent medium in our case because 200 nm was the optimal wavelength in this experiment, while the UV absorption of the amide-type solvents was higher than 240 nm. As a result, MeOH and ACN were chosen as the solvent medium.…”

Section: Sc-ª-cd Systemmentioning

confidence: 76%

“…The first publications on chiral separation by NACE appeared in the literature in 1996 [20][21][22][23]. One of the most important advantages of NACE is the possibility of investigating non or poorly watersoluble substances.…”

Section: Introductionmentioning

confidence: 99%

Nonaqueous and aqueous‐organic media for the enantiomeric separations of neutral organophosphorus pesticides by CE

Lin

LiangJun

et al. 2007

Electrophoresis

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The enantiomeric separation of some poorly water-soluble organophosphorus pesticides (OPs) has been investigated using nonaqueous solvent and aqueous-organic solvent systems. In this work, sodium cholate (SC) either with SDS or gamma-CD was used to achieve enantiomeric separations of four neutral and poorly water-soluble OPs, i.e., profenofos, prothiofos, sulprofos, and pyraclofos. Electrophoretic medium consisted of a mixture of methanol (MeOH) with ACN (4:1 v/v) or a mixture of MeOH with H(2)O and ACN (5:4:1 v/v/v). On one hand, NACE was applied for enantiomeric separation of pyraclofos using a large amount of chiral and achiral surfactants (SC and SDS). On the other hand, H(2)O was added to act as a solvent additive to increase the solubility of gamma-CD in the organic solvents such as MeOH and ACN, in which the solubility of gamma-CD was very low. The presence of H(2)O was found to be particularly useful for the enantiomeric separation of profenofos, prothiofos, and sulprofos. In this way, the range of application of the neutral CDs in CE has been extended. In addition, SC was used as the only electrolyte. The proposed method has been applied for the analysis of soil samples.

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“…Ainsi la quinine a été utilisée dans le méthanol comme sélecteur chiral pour séparer les acides aminés énantiomères dérivatisés par le chlorure de 3,5-dinitrobenzoyle [24]. Par ailleurs des acides aminés dansylés ont été séparés aisément dans le N-méthylformamide à l'aide de β-cyclodextrine comme sélecteur chiral, qui s'avère 35 fois plus soluble en milieu organique qu'en milieu aqueux [25].…”

Section: Solubilitéunclassified

Électrophorèse capillaire en milieu non aqueux

Desbène-Monvernay¹,

Mofaddel²

1999

Analusis

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Chiral separation of dansyl-amino acids in a nonaqueous medium by capillary electrophoresis

Cited by 85 publications

References 53 publications

Effects of organic solvents on sample pretreatment and separation performances in capillary electrophoresis

Effects of organic solvents on sample pretreatment and separation performances in capillary electrophoresis

Nonaqueous and aqueous‐organic media for the enantiomeric separations of neutral organophosphorus pesticides by CE

Électrophorèse capillaire en milieu non aqueux

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