Chiral Separation of Pharmaceuticals by High Performance Liquid Chromatography

Abstract

The increasing demand for enantiopure drugs has led to the development of a variety of stereoselective separation technologies. Among them, high performance liquid chromatography (HPLC) is well recognized as a powerful, fast and efficient technique, which has been successfully employed for analysis and preparation of enantiomers of drugs. Nowadays, liquid chromatographic techniques are the focus of intensive research, which lead to the rational design and production of highly selective and efficient chromatographic materials. This review focuses on various HPLC methods and related hyphenated techniques (including LC-MS, LC-OR detector, LC-CD detector) for chiral separation of pharmaceuticals, many new developments and applications are introduced in chiral HPLC separations in recent years. In this paper, the usage of new materials as chiral stationary phases (CSPs) in liquid chromatography for enantiomeric discrimination is investigated in detail. Moreover, hyphenated techniques are very useful for chiral separation of HPLC, especially for separation of new pharmaceuticals.

HPLC determination of enantiomeric thiazolidine-2-carboxylic acid on chiral stationary phase with pre-column derivatization

“…Thus, an efficient and economic method is necessary to be developed for precise determination of enantiomeric excess (e.e.%) of the thiazolidine-2-carboxylic acid enantiomers. Among the available methods for the separation of enantiomers, the HPLC method with chiral stationary phases (CSPs) is more rapid and efficient in terms of resolution [13][14][15][16]. Our experimental results indicated that thiazolidine-2-carboxylic acid can not be separated directly on the popular CSPs satisfactorily.…”

Design, preparation and application of a Pirkle-type chiral stationary phase for enantioseparation of some racemic organic acids and molecular dynamics studies

“…Among the methods used for the chiral resolution, enantio separation by HPLC using CSPs is currently the most popular one. [7][8][9][10][11][12] For this reason, significant effort was devoted to the development of new effective CSPs in recent years. For this purpose, various kinds of CSPs have been developed and tested so far.…”

Interactions between pyrazole derived enantiomers and Chiralcel OJ: Prediction of enantiomer absolute configurations and elution order by molecular dynamics simulations

“…Although many chiral compounds can be separated by HPLC effectively [13,14], the absolute configurations of the isomers cannot be confirmed directly [15]. e.g.…”