Chiral separation using cyclodextrins as mobile phase additives in open‐tubular liquid chromatography with a pseudophase coating

Abstract

The chiral separation of various analytes (dichlorprop, mecoprop, ibuprofen, and ketoprofen) was demonstrated with different cyclodextrins as mobile phase additives in open-tubular liquid chromatography using a stationary pseudophase semipermanent coating. The stable coating was prepared by a successive multiple ionic layer approach using poly(diallyldimethylammonium chloride), polystyrene sulfonate, and didodecyldimethyl ammonium bromide. Increasing concentrations (0-0.2 mM) of various native and derivatized cyclodextrins in 25 mM sodium tetraborate (pH 9.2) were investigated. Chiral separation was achieved for the four test analytes using 0.05-0.1 mM β-cyclodextrin (resolution between 1.11 and 1.34), γ-cyclodextrin (resolution between 0.78 and 1.27), carboxymethyl-β-cyclodextrin (resolution between 1.64 and 2.59), and 2-hydroxypropyl-β-cyclodextrin (resolution between 0.71 and 1.76) with the highest resolutions obtained with 0.1 mM carboxymethyl-β-cyclodextrin. %RSD values were <10%. This is the first demonstration of chiral open-tubular liquid chromatography using achiral chromatographic coatings and cyclodextrins as mobile phase additives.

Dynamic in situ growth of bonded-phase silica nanospheres on silica capillary inner walls for open-tubular liquid chromatography

“…The functionalized column was also applied to evaluate the tryptic digest of BSA, containing a mixture of numerous peptides in a complex matrix. To have a basis for comparison of the separation performance, a normal silica capillary was modified with poly(diallyldimethylammonium) chloride/polystyrene sulfonate (PDDA-PSS) [ 59 ] and tested under the same condition. PSS would create a negatively charged surface as the partial negative charged surface resulted from the unbonded silanol groups in an SNS-OTS-coated capillary.…”

Recent applications and chiral separation development based on stationary phases in open tubular capillary electrochromatography (2019–2022)

“…CD [ 34 , 35 , 100 ] is the most commonly used polysaccharide material for chiral separations and has appeared frequently. It is either a component of a mixed SP or a chiral selective agent used for synergistic separations.…”

Preparation of open tubular capillary column covalently coated with polystyrene sulfonate with 4,4′‐Azobis(4‐cyanopentanoyl chloride) as polymerization initiator for electrochromatographic separation of alkaloids, sulfonamides, and peptides

“…In previously published work for the preparation of OT columns using polystyrene sulfonate (PSS) as a stationary phase, a dynamic coating (layer-by-layer assembly) procedure was usually used [9,[20][21]. Although ease of preparation is the advantage but the major disadvantage is that the coating gets washed away over time.…”