Chitin deacetylase 1 and 2 are indispensable for larval–pupal and pupal–adult molts in Heortia vitessoides (Lepidoptera: Crambidae)

Wang, Chunyan; Cheng, Jie; Lyu, Zihao; Li, Zhixing; Chen, Jingxiang; Lin, Tong

doi:10.1016/j.cbpb.2019.110325

Cited by 16 publications

(19 citation statements)

References 39 publications

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“…Developmental expression analyses indicated that LsCDA1 was highly expressed in late larval and late pupal stages, which are specific periods prior to each molt. Similar results have been confirmed for many CDA1 genes of other insect species, such as N. lugens [10], T. castaneum [13], S. paniceum [14], and H. vitessoides [27]. The dramatically increased expression of CDA1 was probably related to the requirement for chitin deposition during molt periods.…”

Section: Characteristics and Expression Profiles Of Lscda1supporting

confidence: 82%

“…Similar findings have been reported for LdCDA1 and LdCDA2 in L. decemlineata; however, the expressions levels of two LdCDAs were inhibited after the application of juvenile hormone (JH) and its analogs, and were stimulated by the silencing of JH synthesis and signaling genes [15,16]. In S. paniceum [14] and H. vitessoides [27], exposure to 20E could activate the expression of CDA genes at various times. Substantial upregulation of six BmCDAs (BmCDA1-BmCDA6) by 20E was observed not only in the larval cuticles and midguts, but also in embryo cell lines of B. mori [25].…”

Section: Transcriptional Regulation Of Lscda1 By 20esupporting

confidence: 80%

“…The silencing of NlCDA1, NlCDA2, and NlCDA4 caused molting failure and high mortality, while nonviable phenotypes were found after injection with double-stranded RNA (dsRNA) for NlCDA3 [10]. Similar molting defects were observed after RNAi of CDA genes in many insect species, including Stegobium paniceum [14], Leptinotarsa decemlineata [15,16], Locusta migratoria [19], Choristoneura fumiferana [22], Hyphantria cunea [24], Bombyx mori [25], and Heortia vitessoides [27]. In Drosophila melanogaster, mutations of DmCDA1 (Serpentine) and DmCDA2 (vermiform) led to limited tracheal elongation and affected the formation of the larval cuticle [20,28,29].…”

Section: Introductionmentioning

confidence: 81%

“…In Oxya chinensis, nymphs injected with dsCDA1 displayed arrested development, and more than 95% died during the molting process [18]. The function of CDA1 in the molting was also studied in S. paniceum [14], L. migratoria [19], and H. vitessoides [27]. Given that CDA1 is essential for insect metamorphosis, it could be a promising target for RNAi-based insecticide development.…”

Section: Knockdown Of Lscda1 Affects Chitin Metabolism and Impairs Momentioning

confidence: 99%

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Role of Chitin Deacetylase 1 in the Molting and Metamorphosis of the Cigarette Beetle Lasioderma serricorne

Yang

Yan

et al. 2020

IJMS

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Chitin deacetylases (CDAs) are chitin-modifying enzymes known to play vital roles in insect metamorphosis and development. In this study, we identified and characterized a chitin deacetylase 1 gene (LsCDA1) from the cigarette beetle Lasioderma serricorne. LsCDA1 contains a 1614 bp open reading frame encoding a protein of 537 amino acids that includes domain structures typical of CDAs. LsCDA1 was mainly expressed in the late larval and late pupal stages. In larval tissues, the highest level of LsCDA1 was detected in the integument. The expression of LsCDA1 was induced by 20-hydroxyecdysone (20E) in vivo, and it was significantly suppressed by knocking down the expression of ecdysteroidogenesis genes and 20E signaling genes. RNA interference (RNAi)-aided silencing of LsCDA1 in fifth-instar larvae prevented the larval–pupal molt and caused 75% larval mortality. In the late pupal stage, depletion of LsCDA1 resulted in the inhibition of pupal growth and wing abnormalities, and the expression levels of four wing development-related genes (LsDY, LsWG, LsVG, and LsAP) were dramatically decreased. Meanwhile, the chitin contents of LsCDA1 RNAi beetles were significantly reduced, and expressions of three chitin synthesis pathway genes (LsTRE1, LsUAP1, and LsCHS1) were greatly decreased. The results suggest that LsCDA1 is indispensable for larval–pupal and pupal–adult molts, and that it is a potential target for the RNAi-based control of L. serricorne.

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Section: Characteristics and Expression Profiles Of Lscda1supporting

confidence: 82%

Section: Transcriptional Regulation Of Lscda1 By 20esupporting

confidence: 80%

Section: Introductionmentioning

confidence: 81%

Section: Knockdown Of Lscda1 Affects Chitin Metabolism and Impairs Momentioning

confidence: 99%

See 2 more Smart Citations

Role of Chitin Deacetylase 1 in the Molting and Metamorphosis of the Cigarette Beetle Lasioderma serricorne

Yang

Yan

et al. 2020

IJMS

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“…A. sinensis is the principal source of Chinese agarwood, a resinous heartwood formed in response to fungal infection, a moth whose leaf-eating larvae use A. sinensis as a sole food source [3]. The moth annually produces up to seven-to eight-generational alternation in southern China, and its larvae entirely defoliate A. sinensis, causing serious financial losses [4][5][6]. Control methods such as avermectin, sex pheromone, and trichlorfon administration have been used for infestations [7].…”

Section: Introductionmentioning

confidence: 99%

Cloning, Expression Analysis, 20-Hydroxyecdysone Induction, and RNA Interference Study of Autophagy-Related Gene 8 from Heortia vitessoides Moore

Lyu

et al. 2020

Insects

Self Cite

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Autophagy is a highly conserved and regulated process in eukaryotic cells and remodels cytoplasm, recovers essential nutrients, and disposes of unwanted cytoplasmic components. Autophagy-related gene (ATG) 8, identified in Heortia vitessoides Moore, which is an oligophagous pest of Aquilaria sinensis (Lour.), was characterized (HvATG8). Multiple sequence alignment showed that HvATG8 possesses highly conserved domain structures. Stage-and tissue-specific expressions indicated that HvATG8 is highly expressed in prepupal, pupal, and adult stages and in the midgut of larvae and abdomen of adults. Lack of function of HvATG8 by RNA interference resulted in a significant decrease in survival rate and an increase in abnormal or nonviable phenotypes in H. vitessoides. Transition rate from larval to pupal stages was 33.0% and from pupal to adult stages was 15.0% after injection. Reduction of ATG8 expression reduced survival of H. vitessoides. Therefore, HvATG8 possibly plays a key role in normal growth stage of H. vitessoides. HvATG8 suppression downregulates HvATG3 expression, suggesting that the two genes are interconnected. Further, HvATG8 expression increased by 20-hydroxyecdysone treatment, starvation, and extreme temperature exposure. Starvation also altered expression of other ATGs in H. vitessoide. This study may be used to guide research on molecular mechanisms of autophagy in insects.Insects 2020, 11, 245 2 of 16 Autophagy is a self-digesting system of cells, which plays a pivotal role in maintaining the stability of the intracellular environment [9]. Three different types have been mentioned to date: microautophagy, chaperone-mediated autophagy, and macroautophagy [10]. Microautophagy means autonomous depression and deformation of lysosomal membranes or vacuolar membranes, which envelop and engulf part of the cytoplasm. An autophagy vesicle has a double-layer membrane structure, and its outer membrane can be fused with the lysosomal membrane. After autophagy vesicles enter the lysosome, the inner membrane is rapidly degraded, and vesicle contents are released, which is degraded by the hydrolytic enzyme; this allows cells to reuse substrates. In chaperone-mediated autophagy, receptors on the surface of lysosomal membranes selectively bind to soluble cytosolic proteins and regulate their transport to lysosomes. The formed chaperone-substrate complex is bound to the receptor of the lysosomal membrane, and the substrate is transported by the secondary chaperone protein in the cavity [11]. Macroautophagy, a degradation process focused on eliminating macromolecules and organelles, is the main pathway of autophagy in eukaryotic cells, which communicates a highly conducted self-degradation process that is started as an adaptive response under adverse conditions, for instance, nutritional removal. Usually, autophagy is referred to as macroautophagy. It is an intracellular process that degrades proteins in cytoplasmic components and organelles using lysosomes in eukaryote organisms ranging from yeast to mammals [11]. Auto...

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Genetic Engineering Technologies for Management of Crambid Pests

Singh

Kalenahalli

Voddagallaiah

2022

Genetic Methods and Tools for Managing Crop Pests

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Chitin deacetylase 1 and 2 are indispensable for larval–pupal and pupal–adult molts in Heortia vitessoides (Lepidoptera: Crambidae)

Cited by 16 publications

References 39 publications

Role of Chitin Deacetylase 1 in the Molting and Metamorphosis of the Cigarette Beetle Lasioderma serricorne

Role of Chitin Deacetylase 1 in the Molting and Metamorphosis of the Cigarette Beetle Lasioderma serricorne

Cloning, Expression Analysis, 20-Hydroxyecdysone Induction, and RNA Interference Study of Autophagy-Related Gene 8 from Heortia vitessoides Moore

Genetic Engineering Technologies for Management of Crambid Pests

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