Chitin synthase 1 (Chs1) gene sequences of Microsporum equinum and Trichophyton equinum

The New Species Concept in Dermatophytes—a Polyphasic Approach

“…Type IV refers to A. benhamiae strains [67,Drouot et al,unpublished data]. RFLP analysis of the mtDNA as well as of the ITS and chitin synthase I sequences were too conservative to discriminate among T. tonsurans, T. interdigitale and T. equinum [35,54,77].…”

“…Though dermatophyte species proved to be relatively closely related to one another, early molecular tests such as restriction fragment typing [28] were able to distinguish common species. Full entry into the modern era of biosystematics came with the first molecular phylogeny of the dermatophytes and their Arthrodermataceous relatives by Gräser et al [29], with additional molecular phylogenetic analyses rapidly following [30][31][32][33][34][35]. All these phylogenies tended to be strongly influenced by the close evolutionary relationships among dermatophyte species that evolved on hosts that are themselves relatively recently evolved, particularly humans as well as animals such as cats, cattle and horses that have undergone rapid selection and habitat change in the course of domestication.…”

A rapid molecular method for diagnosing epidemic dermatophytosis in a racehorse facility

“…The dermatophyte genomic DNA samples (200 ng) were amplified by PCR in a reaction mixture (30 µ l) containing 10 mmol/l Trishydrochloride (pH 8.3), 50 mmol/l KCl, 1.5 mmol/l MgCl 2 , 0.001% gelatin, 200 mmol/l of each deoxynucleoside triphosphate (dNTP), 1.0 unit of rTaq DNA polymerase 3 , and 0.5 µ g of primer pairs. The sequences of the specific CHS1 gene primers in dermatophytes were based on the sequences of previous research (Kano et al. 1997, 2001, 2003).…”

Fungal Skin Diseases

“…9 Extremely rare isolates include T. tonsurans, T. rubrum, M. nanum, M. audouinii, M. cookei, T. terrestre, T. schoenleinii, M. distortum, and Epidermophyton floccosum. 19 The incidence and prevalence of dermatophytosis vary with the climate, natural reservoirs, and living conditions. 9 Phylogenetic analyses indicate that T. equinum and M. equinum are closely related to the teleomorphs Arthroderma vanbreuseghemii and A. otae, respectively.…”