Chitinase and β-1,3-glucanase activities of Trichoderma harzianum in response towards pathogenic and non-pathogenic isolates: Early indications of compatibility in consortium

Ting, Adeline Su Yien; Chai, Jing Yun

doi:10.1016/j.bcab.2014.10.003

Cited by 30 publications

(13 citation statements)

References 34 publications

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“…Chitinase activity is essential for the selection of an efficient biocontrol agent since chitin is the major structural component of cell wall from pathogens. Chitinases together with β-1,3-glucanases are cell-wall-degrading enzymes involved in the initiation of mycoparasitism [42,43]. The secreted enzymes act synergistic each other, but at the same time with other metabolites.…”

Section: Discussionmentioning

confidence: 99%

Applications of Fungal Strains with Keratin-Degrading and Plant Growth Promoting Characteristics

et al. 2019

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Protein hydrolysates (PHs) are organic non-microbial biostimulants having beneficial effects on plants. The study was designed to assess the effects on plants by the applications of PHs obtained from Trichoderma isolates grown on keratin wastes. Trichoderma isolates were characterized for indole-3-acetic acid and siderophores production, activity of lytic enzymes, phosphorous solubilization and inhibition of pathogens growth, using qualitative specific tests. Fungal isolates were cultured on a medium with keratin wastes (wool and feathers) to obtain PHs. Fungal PHs were tested in vivo for plant biostimulant action, as follows: (i) seeds germination test; (ii) activation of plant proton pump; (iii) evaluation of effect on tomato seedling growth. PHs from T. asperellum cultured on feathers medium reached the highest values for all parameters recorded (plant height and diameter, number of leaves and branches), with the exception of those for plant biomass, which were maximum for the wool medium. The metabolites released by keratin degradation under the activity of selected T. asperellum isolate improved crop health and productivity. The use of PHs can be a reasonable solution for the environmental pollution of by-products from the food chain, as well as for the replacement of chemical fertilizers with microbial formulations to stimulate plant growth.

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Section: Discussionmentioning

confidence: 99%

Applications of Fungal Strains with Keratin-Degrading and Plant Growth Promoting Characteristics

et al. 2019

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“…Of the 61 isolates, 17 presented high chitinase activity and 8 presented medium chitinase activity. Ting and Chai (2015) evaluated the expression of chitinase and β-1,3-glucanase activity of T. harzianum in the presence of pathogenic isolates (Fusarium oxysporum, Ganoderma boninense) and non-patogenics (T. viridescens, Serratia marcescens, and Streptomyces griseus). The results showed that T. harzianum presented chitinase activity, independent of the presence of pathogen isolates, as it produces these enzymes naturally.…”

Section: Shelf Lifementioning

confidence: 99%

Encapsulation of Trichoderma harzianum Preserves Enzymatic Activity and Enhances the Potential for Biological Control

Maruyama

Bilesky-José

Lima

et al. 2020

Front. Bioeng. Biotechnol.

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Trichoderma harzianum is a biological control agent used against phytopathogens and biostimulation in agriculture. However, its efficacy can be affected by biotic and abiotic factors, and microencapsulation has been used to maximize the efficacy. The objective was to develop polymeric microparticles to encapsulate T. harzianum, to perform physicochemical characterization to evaluate its stability, to evaluate effects on the soil microbiota, antifungal activity in vitro and enzymatic activity. Size distribution of wet and dry microparticles was 2000 and 800 µm, respectively. Scanning electron microscopy showed spherical morphology and encapsulation of T. harzianum. Photostability assays showed that encapsulation protected the fungus against ultraviolet radiation. The evaluation of the microbiota showed that the proportion of denitrifying bacteria increased when compared to the control. The T. harzianum encapsulation showed an improvement in the chitinolytic and cellulosic activity. In vitro tests showed that encapsulated fungus were able to provide a greater control of S. sclerotiorum.

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“…β-N-Acetylglucosaminidase (EC 3.2.1.52) is a subcategory of exochitinase; they hydrolyze short N-acetyl GOSs, typically N-acetyl chitobiose to release N-acetylglucomine (GlcNAc) as the solo end product. 3,5 Chitinases are widely distributed in different organisms, including bacteria, 4,6 fungi, 7,8 insects, 9 animals, 10 and plants. 11,12 Among them, microbial chitinases have gained more research interest because they may be easily produced and put into commercial use.…”

Section: ■ Introductionmentioning

confidence: 99%

“…levisporus, 18 Paecilomyces thermophila, 7 Penicillium ochrochloron, 17 Metschnikowia f ructicola, 15 Thermomyces lanuginosus, 16 and Trichoderma harzianum. 8 No chitinase has been ever reported from the thermophilic fungus, Rhizomucor miehei.…”

Section: ■ Introductionmentioning

confidence: 99%

Biochemical Characterization of a Novel Acidic Exochitinase from Rhizomucor miehei with Antifungal Activity

Yang

Yan

et al. 2016

J. Agric. Food Chem.

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A novel chitinase gene (RmChi44) from Rhizomucor miehei was cloned and expressed in Escherichia coli as an intracellular soluble and active protein. The recombinant chitinase (RmChi44) was purified to homogeneity and biochemically characterized. The molecular mass of RmChi44 was estimated to be 44.6 kDa on SDS-PAGE. RmChi44 displayed an acidic pH optimum of 4.5 and was stable within pH 4.5-9.0. The optimal temperature of RmChi44 was found to be 50 °C. The Km values of RmChi44 for colloidal chitin and glycol chitin were 4.02 and 1.55 mg/mL, respectively. RmChi44 hydrolyzed colloidal chitin to yield mainly N-acetyl chitobiose, exhibiting an exotype cleavage pattern. Moreover, the enzyme displayed β-N-acetylglucosaminidase activity, splitting N-acetyl COSs with degree of polymerization (DP) 2-5 into their monomer. In addition, RmChi44 showed antifungal activity against some phytopathogenic fungi. This is the first report on an exochitinase showing β-N-acetylglucosaminidase activity and antifungal activity from Rhizomucor species.

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Chitinase and β-1,3-glucanase activities of Trichoderma harzianum in response towards pathogenic and non-pathogenic isolates: Early indications of compatibility in consortium

Cited by 30 publications

References 34 publications

Applications of Fungal Strains with Keratin-Degrading and Plant Growth Promoting Characteristics

Applications of Fungal Strains with Keratin-Degrading and Plant Growth Promoting Characteristics

Encapsulation of Trichoderma harzianum Preserves Enzymatic Activity and Enhances the Potential for Biological Control

Biochemical Characterization of a Novel Acidic Exochitinase from Rhizomucor miehei with Antifungal Activity

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