Chitinases as Antibacterial Proteins: A Systematic Review

Medeiros, Suelen Carneiro de; Júnior, José Telmo Valença; Sales, Gleilton Weyne Passos; Grangeiro, Thalles Barbosa; Nogueira, Nádia Accioly Pinto

doi:10.5530/jyp.2018.10.33

Cited by 21 publications

(15 citation statements)

References 38 publications

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“…In particular, one defense-related gene, e ndochitinase B , was differentially expressed and highly upregulated in resistant hybrids (see Supplementary Table S6 ). Endochitinases have previously been reported as important bactericides, and some of them have ability to cleave peptidoglycan chains, promoting bacterial cell lysis 13 . Other defense-related genes were differentially expressed in susceptible plants by CLas.…”

Section: Resultsmentioning

confidence: 99%

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Wide-ranging transcriptomic analysis of Poncirus trifoliata, Citrus sunki, Citrus sinensis and contrasting hybrids reveals HLB tolerance mechanisms

Curtolo

Pacheco

Boava

et al. 2020

Sci Rep

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Huanglongbing (HLB), caused mainly by ‘Candidatus Liberibacter asiaticus’ (CLas), is the most devastating citrus disease because all commercial species are susceptible. HLB tolerance has been observed in Poncirus trifoliata and their hybrids. A wide-ranging transcriptomic analysis using contrasting genotypes regarding HLB severity was performed to identify the genetic mechanism associated with tolerance to HLB. The genotypes included Citrus sinensis, Citrus sunki, Poncirus trifoliata and three distinct groups of hybrids obtained from crosses between C. sunki and P. trifoliata. According to bacterial titer and symptomatology studies, the hybrids were clustered as susceptible, tolerant and resistant to HLB. In P. trifoliata and resistant hybrids, genes related to specific pathways were differentially expressed, in contrast to C. sinensis, C. sunki and susceptible hybrids, where several pathways were reprogrammed in response to CLas. Notably, a genetic tolerance mechanism was associated with the downregulation of gibberellin (GA) synthesis and the induction of cell wall strengthening. These defense mechanisms were triggered by a class of receptor-related genes and the induction of WRKY transcription factors. These results led us to build a hypothetical model to understand the genetic mechanisms involved in HLB tolerance that can be used as target guidance to develop citrus varieties or rootstocks with potential resistance to HLB.

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Section: Resultsmentioning

confidence: 99%

“…Interestingly, the exclusive DEGs of the R Pool, formed by the CLas-negative hybrids, may be linked with genes and mechanisms capable of eliminating the bacteria from the plant, such as endochitinase B . Plant endochitinases cleave peptidoglycan chains, thereby promoting bacterial cell lysis 13 .…”

Section: Discussionmentioning

confidence: 99%

Wide-ranging transcriptomic analysis of Poncirus trifoliata, Citrus sunki, Citrus sinensis and contrasting hybrids reveals HLB tolerance mechanisms

Curtolo

Pacheco

Boava

et al. 2020

Sci Rep

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“…The gene annotation of mannan synthase [51], vacuolar protein sorting-associated protein [52], auxin efflux carrier protein [53] and cytochrome P450 [54] were related to plant growth and development, and the gene annotation of glycosyltransferase family 2 protein [55] and 3-ketoacyl-CoA synthase [56] were related to plant resistance. In the five descending genes, the three genes, ferritin [57], anthranilate N -benzoyltransferase protein [58] and chitinase protein [59] were related to antibacterial activity. The results indicated that the growth and stress resistance of tetraploid plants was better than those of diploid ones, while their antibacterial ability was worse than diploid ones.…”

Section: Resultsmentioning

confidence: 99%

Induction, identification and genetics analysis of tetraploid Actinidia chinensis

Liu

et al. 2019

R. Soc. open sci.

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Actinidia chinensis is a commercially important fruit, and tetraploid breeding of A. chinensis is of great significance for economic benefit. In order to obtain elite tetraploid cultivars, tetraploid plants were induced by colchicine treatment with leaves of diploid A. chinensis ‘SWFU03’. The results showed that the best treatment was dipping leaves 30 h in 60 mg l−1 colchicine solutions, with induction rate reaching 26%. Four methods, including external morphology comparison, stomatal guard cell observation, chromosome number observation and flow cytometry analysis were used to identify the tetraploid of A. chinensis. Using the induction system and flow cytometry analysis methods, 187 tetraploid plants were identified. Three randomly selected tetraploid plants and their starting diploid plants were further subjected to transcriptome analysis, real-time quantitative polymerase chain reaction (RT-qPCR) and methylation-sensitive amplification polymorphism (MSAP) analysis. The transcriptome analysis results showed that there were a total of 2230 differentially expressed genes (DEG) between the diploid and tetraploid plants, of which 660 were downregulated and 1570 upregulated. The DEGs were mainly the genes involved in growth and development, stress resistance and antibacterial ability in plants. RT-qPCR results showed that the gene expression levels of the growth and stress resistance of tetraploid plants were higher than those of diploid ones at the transcriptome level. MSAP analysis of DNA methylation results showed that tetraploid plants had lower methylation ratio than diploid ones. The present results were valuable to further explore the epigenetics of diploid and tetraploid kiwifruit plants.

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“…Few of the derivatives of chitin are famously known to be non-allergic, nontoxic, biocompatible, and biodegradable due to the huge medical use of these kinds of compounds. Chitinases also have some other medical applications as well, such as the first invention of the involvement of acidic mammalian chitinase (AMCase) in the pathogenesis of asthma was unexpected and novel because mammals did not have the ability to use chitin as a source of energy, nor they generate any structure of chitinase [61][62][63][64][65][66][67][68][69][70][71][72][73][74][75].…”

Section: Medicinal Functionsmentioning

confidence: 99%

Chitinases Potential as Bio-Control

Iqbal

2019

BJSTR

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The richest second polysaccharide in nature later than cellulose is chitin. It is present in the exoskeleton of yeast, fungi, algae and insects. It is also present in the inner structure of different vertebrates. The degradation of chitin is done by chitinases enzymes. In the environment nitrogen and carbon are generated by the contribution of chitinases. In biotechnological applications chitinolytic enzymes and chitin have gaining their significance. In the field of agriculture for the control of pathogens particularly the chitinases demoralized. Some human diseases particularly such as asthma, chitinases have a broad use for human health care. For the preparation of pharmaceutically significant chito-oligosaccharides and preparation of single cell protein, N-acetyl D glucosamine, separation of protoplast from yeast & fungi, for the handling of chitinous squander, to stop the pathogenic fungi and to stop the morphogenesis and mosquitoes etc chitinases has wider range applications. We are discussed a different type of chitinases and the chitinases present in a number of organisms for example plants, bacteria, mammals and fungi in this review.

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Chitinases as Antibacterial Proteins: A Systematic Review

Cited by 21 publications

References 38 publications

Wide-ranging transcriptomic analysis of Poncirus trifoliata, Citrus sunki, Citrus sinensis and contrasting hybrids reveals HLB tolerance mechanisms

Wide-ranging transcriptomic analysis of Poncirus trifoliata, Citrus sunki, Citrus sinensis and contrasting hybrids reveals HLB tolerance mechanisms

Induction, identification and genetics analysis of tetraploid Actinidia chinensis

Chitinases Potential as Bio-Control

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