Chitinases biosynthesis by immobilized Aeromonas hydrophila SBK1 by prawn shells valorization and application of enzyme cocktail for fungal protoplast preparation

Halder, Suman Kumar; Maity, Chiranjit; Jana, Arijit; Ghosh, Kuntal; Das, Asim K.; Paul, Tanmay; Mohapatra, Pradeep Kumar Das; Pati, Biswamoy; Mondal, Keshab Chandra

doi:10.1016/j.jbiosc.2013.07.011

Cited by 24 publications

(7 citation statements)

References 18 publications

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“…In time less than 5 h enzyme molecules bind inefficiently to the support and hence decreased laccase activity was observed. 26 There was decreasing enzyme activity with increasing immobilization time beyond the optimum. It is possible that over time as the quantity of enzyme adsorbed onto the support increased it led to overcrowding, thus decreasing the enzyme activity.…”

Section: Effect On Enzyme Dosementioning

confidence: 97%

“…It is possible that over time as the quantity of enzyme adsorbed onto the support increased it led to overcrowding, thus decreasing the enzyme activity. 26 In another study, the optimal immobilization time of laccase from T. pubescens was reported to be 4 h. 9 Characterization of chitosan-immobilized laccase Immobilization of an enzyme is known to affect its characteristics with respect to temperature, pH and other external parameters. 9 Therefore, the activity profile of the immobilized LAC-DS was studied with respect to these parameters.…”

Section: Effect On Enzyme Dosementioning

confidence: 99%

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Immobilization of laccase from Bacillus subtilisDS on chitosan beads and standardization of process for biodegradation of textile dyes

Kumar

Khullar

Sharma

et al. 2021

J of Chemical Tech & Biotech

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BACKGROUND:The textile industry generates a huge amount of effluents containing different hazardous dyes. Indigo carmine and malachite green are the most commonly used dyes in textile and dye industries. Pollution caused by these dyes affects aquatic life.RESULTS: Bacillus subtilis DS produces an alkali-thermostable laccase. This enzyme was immobilized on chitosan beads and conditions for immobilization were optimized. After immobilization, the maximum activity of 26 IU g -1 beads was found under optimized conditions of 1% glutaraldehyde, 3 h cross-linking time and 5 h immobilization time. The immobilized enzyme retained a temperature and pH profile similar to that of the free enzyme. Significant enzyme activity was retained after ten reaction cycles with >60% stability after 15 days, allowed reuse of enzyme over multiple cycles. Immobilized laccase was used to degrade textile dyes and the conditions were standardized statistically for its optimal degradation. Under optimized conditions a high degradation rate was achieved for indigo carmine (95.24%) and malachite green (90%), respectively. CONCLUSION: Immobilized laccase is a highly suitable candidate for bioremediation of textile industry dyes because of its stability and reusability.

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Section: Effect On Enzyme Dosementioning

confidence: 97%

Section: Effect On Enzyme Dosementioning

confidence: 99%

Immobilization of laccase from Bacillus subtilisDS on chitosan beads and standardization of process for biodegradation of textile dyes

Kumar

Khullar

Sharma

et al. 2021

J of Chemical Tech & Biotech

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“…Efficient cell wall degradation enzymes are of substantial importance to generate protoplasts. Some degradation enzymes, such as the snailase (Mink et al, 1990) and chitinase (Patil et al, 2013;Halder et al, 2014), have been frequently used to deconstruct the hyphae in some fungi. Shi et al (2019) performed a series of optimizations on the preparation of protoplasts.…”

Section: Protoplast Preparation and Transformation Technologiesmentioning

confidence: 99%

The Gibberellin Producer Fusarium fujikuroi: Methods and Technologies in the Current Toolkit

Cen

Lin

Wang

et al. 2020

Front. Bioeng. Biotechnol.

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In recent years, there has been a noticeable increase in research interests on the Fusarium species, which includes prevalent plant pathogens and human pathogens, common microbial food contaminants and industrial microbes. Taken the advantage of gibberellin synthesis, Fusarium fujikuroi succeed in being a prevalent plant pathogen. At the meanwhile, F. fujikuroi was utilized for industrial production of gibberellins, a group of extensively applied phytohormone. F. fujikuroi has been known for its outstanding performance in gibberellin production for almost 100 years. Research activities relate to this species has lasted for a very long period. The slow development in biological investigation of F. fujikuroi is largely due to the lack of efficient research technologies and molecular tools. During the past decade, technologies to analyze the molecular basis of host-pathogen interactions and metabolic regulations have been developed rapidly, especially on the aspects of genetic manipulation. At the meanwhile, the industrial fermentation technologies kept sustained development. In this article, we reviewed the currently available research tools/methods for F. fujikuroi research, focusing on the topics about genetic engineering and gibberellin production.

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“…Chitin, the second most abundant natural polymer, is a nitrogen-containing polysaccharide composed of a β-(1,4)-linked N -acetyl-D-glucosamine (GlcNac) [ 1 , 2 ]. Chitin is widely distributed as a structural component of crustaceans, insects, and other arthropods and as a component of most fungi and some algae cell walls.…”

Section: Introductionmentioning

confidence: 99%

“…Approximately 75% of the total weight of shellfish, such as shrimp, crabs, and krill, is considered waste, and chitin comprises 20–58% of the dry weight of the said waste [ 3 ]. Annually, more than 1000 metric tons of chitin are synthesized in the aquatic biosphere and deposited in marine sediment; thus, chitinolytic bacteria efficiently degrade this material to maintain the balance in the marine ecosystem [ 1 , 4 ]. Bacteria can synthesize chitinases to hydrolyze chitin as a carbon, nitrogen, and energy source, consequently making possible the recycling of chitin in the environment [ 2 , 5 ].…”

Section: Introductionmentioning

confidence: 99%

Endochitinase and Chitobiosidase Production by Marine Aeromonas caviae CHZ306: Establishment of Nitrogen Supplementation

et al. 2023

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Aeromonas caviae CHZ306, a marine-derived bacterium isolated from zooplankton, can use chitin (a polymer of a β-(1,4)-linked N-acetyl-D-glucosamine) as a carbon source. The chitin is hydrolyzed by chitinolytic enzymes, namely endochitinases and exochitinases (chitobiosidase and N-acetyl-glucosaminidase). Indeed, the chitinolytic pathway is initiated by the coexpression of the enzymes endochitinase (EnCh) and chitobiosidase (ChB); however, few studies, including biotechnological production of these enzymes, have been reported, although chitosaccharide are helpful in several industries, such as cosmetics. This study demonstrates the potential to maximize the simultaneous EnCh and ChB production by nitrogen supplementation on culture media. Twelve different nitrogen supplementation sources (inorganic and organic) previously analyzed in elemental composition (carbon and nitrogen) were tested and evaluated in the Erlenmeyer flask culture of A. caviae CHZ306 for EnCh and ChB expression. None of the nutrients inhibited bacterial growth, and the maximum activity in both EnCh and ChB was observed at 12 h, using corn-steep solids and peptone A. Corn-steep solids and peptone A were then combined at three ratios (1:1, 1:2, and 2:1) to maximize the production. The high activities for EnCh (30.1 U.L−1) and ChB (21.3 U.L−1) were obtained with 2:1 corn-steep solids and peptone A, corresponding to more than 5- and 3-fold enhancement, respectively, compared to the control condition.

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Chitinases biosynthesis by immobilized Aeromonas hydrophila SBK1 by prawn shells valorization and application of enzyme cocktail for fungal protoplast preparation

Cited by 24 publications

References 18 publications

Immobilization of laccase from Bacillus subtilisDS on chitosan beads and standardization of process for biodegradation of textile dyes

Immobilization of laccase from Bacillus subtilisDS on chitosan beads and standardization of process for biodegradation of textile dyes

The Gibberellin Producer Fusarium fujikuroi: Methods and Technologies in the Current Toolkit

Endochitinase and Chitobiosidase Production by Marine Aeromonas caviae CHZ306: Establishment of Nitrogen Supplementation

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