Chitinimonas arctica sp. nov., isolated from Arctic tundra soil

Abstract

<jats:p>A Gram-stain-negative, rod-shaped, green-pigmented, aerobic and motile bacterium, strain R3-44<jats:sup>T</jats:sup>, was isolated from Arctic tundra soil. Stain R3-44<jats:sup>T</jats:sup> clustered closely with members of the genus <jats:italic> <jats:named-content content-type="genus"> <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.8539" xlink:type="simple">Chitinimonas</jats:ext-link> </jats:named-content> </jats:italic>, which belongs to the family <jats:italic> <jats:named-content content-type="family"> <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.1618" xlink:type="simple">Burkholderiaceae</jats:ext-link> </jats:named-content> </jats:italic>, and showed the highest 16S rRNA sequence similarity to <jats:italic> <jats:named-content content-type="species"> <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.30766" xlink:type="simple">Chitinimonas naiadis</jats:ext-link> </jats:named-content> </jats:italic> AR2<jats:sup>T</jats:sup> (96.10%). Strain R3-44<jats:sup>T</jats:sup> grew optimally at pH 7.0, 28 °C and in the presence of 0–0.5 % (w/v) NaCl. The predominant respiratory isoprenoid quinone of strain R3-44<jats:sup>T</jats:sup> was identified as ubiquinone Q-8. The polar lipids consisted of phosphatidylglycerol, phosphatidylethanolamine, unidentified aminolipid and unidentified phospholipid. The main fatty acids were summed feature 3 (comprising C<jats:sub>16 : 1</jats:sub> <jats:italic> </jats:italic>ω7<jats:italic>c</jats:italic> and/or C<jats:sub>16 : 1</jats:sub> <jats:italic> </jats:italic>ω6<jats:italic>c</jats:italic>, 40.6 %) and C<jats:sub>16 : 0</jats:sub> (29.3 %). The DNA G+C content of strain R3-44<jats:sup>T</jats:sup> was 60.8 mol%. On the basis of the evidence presented in this study, strain R3-44<jats:sup>T</jats:sup> represents a novel species of the genus <jats:italic> <jats:named-content content-type="genus"> <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.8539" xlink:type="simple">Chitinimonas</jats:ext-link> </jats:named-content> </jats:italic>, for which the name <jats:italic>Chitinimonas arctica</jats:italic> sp. nov. is proposed, with the type strain R3-44<jats:sup>T</jats:sup> (=CCTCC AB 2010422<jats:sup>T</jats:sup>=KCTC 72602<jats:sup>T</jats:sup>).</jats:p>

Parachitinimonas caeni gen. nov., sp. nov., a novel member of family Burkholderiaceae isolated from activated sludge collected in Shenzhen, PR China

“…The pH value was adjusted before sterilization to pH 3.0-10.0 (at intervals of 0.5 pH units) in R2A broth using appropriate biological buffers. The temperature range for growth was determined on R2A plates by incubating the cultures at 4-45 °C (4,10,15,20,25,28,30,37,40,42 and 45 °C) for 3 days and at 4 °C for at least 14 days. The following phenotypic tests were performed according to standard methods [19]: catalase and oxidase activities; nitrate reduction; and hydrolysis of starch, carboxymethyl cellulose, gelatin, Tween 20, Tween 40 and Tween 80.…”

“…The genus Chitinimonas was first isolated from a shrimp-farming pond and proposed to represent a distinct genus within the family Burkholderiaceae with the unique ability to degrade chitin [9]. Currently, seven species have been recognized within the genus Chitinimonas : Chitinimonas taiwanensis [9], Chitinimonas koreensis [10], Chitinimonas viridis [11], Chitinimonas prasina [12], Chitinimonas naiadis [13], Chitinimonas lacunae [14] and Chitinimonas arctica [15]. The genus Chitinivorax was first isolated from a freshwater lake; only one species has been recognized within the genus Chitinivorax , namely Chitinivorax tropicus [16].…”