Chitinophaga parva sp. nov., a new member of the family Chitinophagaceae, isolated from soil in a chemical factory

Abstract

A Gram-stain-negative bacterium, designated LY-1, was isolated from the soil sample collected from a chemical factory in Fuyang city, Anhui province, China. Cells of strain LY-1 were strictly aerobic, non-motile and rod-shaped. Strain LY-1 grew optimally at pH 7.0 and at 30-35 °C. The taxonomic position was investigated using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain LY-1 was a member of the genus Chitinophaga and showed the highest sequence similarity to Chitinophaga costaii A37T2 (97.5 %) and lower (<97.0 %) sequence similarity to other known Chitinophaga species. Chemotaxonomic analysis revealed that strain LY-1 possessed menaquinone-7 as the major isoprenoid quinone; and iso-C15 : 0 (46.4 %), C16 : 1ω5c (27.8 %) and iso-C17 : 0 3-OH (9.0 %) were the predominant fatty acids. The polar lipids of strain LY-1 consisted of phosphatidylethanolamine, three unidentified phosphoaminolipids, one unidentified phospholipid, four unidentified lipids, two unidentified aminolipids and two unidentified glycolipids. The genomic DNA G+C content of strain LY-1 was 52.4 mol% based on total genome calculations. The average nucleotide identity and the digital DNA-DNA hybridization value of the draft genomes between strain LY-1 and strain A37T2 were 76.8 and 19.8 %, respectively. Based on the phylogenetic and phenotypic characteristics, chemotaxonomic data, and DNA-DNA hybridization, strain LY-1 is considered a novel species of the genus Chitinophaga, for which the name Chitinophagaparva sp. nov. (type strain LY-1=CCTCC AB 2018018=KCTC 62444) is proposed.

Paraflavisolibacter caeni gen. nov., sp. nov., a novel taxon within the family Chitinophagaceae isolated from sludge

“…tropicus LCS9 T , iso-C 15 : 1 G and anteiso-C 15 : 1 A were the major fatty acids in LCS9 T , but not detected in strain LB-8 T (). The only respiratory quinone of strain LB-8 T was menaquinone-7 (MK-7), which is also reported for the genera Flavisolibacter , Paracnuella , Cnuella and other members of the family Chitinophagaceae [1, 4–8, 29–31]. The major polar lipids of strain LB-8 T were phosphatidylethanolamine, four unidentified aminolipids and two unidentified lipids (Fig.…”

“…However, strain LB-8 T could be differentiated from its near relatives by producing large amount of anteiso-C 15 : 0 and failing to produce iso-C 15 : 1 G. Compared to its closest relative F. tropicus LCS9 T , iso-C 15 : 1 G and anteiso-C 15 : 1 A were the major fatty acids in LCS9 T , but not detected in strain LB-8 T (Table 2). The only respiratory quinone of strain LB-8 T was menaquinone-7 (MK-7), which is also reported for the genera Flavisolibacter, Paracnuella, Cnuella and other members of the family Chitinophagaceae [1,[4][5][6][7][8][29][30][31].…”

“…According to the List of Prokaryotic Names with Standing in Nomenclature (https://lpsn.dsmz.de/family/chitinophagaceae) [2], there are 53 validly published species (including synonyms) in the family Chitinophagaceae with Chitinophaga [3] as the type genus. Members of the family Chitinophagaceae are Gram-negative, aerobic or facultatively anaerobic, usually non-motile, rod-shaped and contain MK-7 as the isoprenoid quinone and iso-C 15 : 0 , iso-C 17 : 0 3-OH and iso-C 15 : 1 G as the major fatty acids [1, 3–8]. During the course of a study on the bacterial diversity in an activated sludge collected from an agricultural chemical factory in Binzhou city, Shandong province, PR China, a pale yellow-pigmented bacterium, named LB-8 T , was isolated.…”

Commonalities between the Atacama Desert and Antarctica rhizosphere microbial communities

“…On the other hand, Haliangum and Gemmatimonas have been proposed as beneficial soil bacteria that tend to be increased in healthy soils ( Gong et al, 2021 ; Shang and Liu, 2021 ). Finally, multiple strains from Chitinophagaceae have been isolated and reported as PGPR ( Madhaiyan et al, 2015 ; Dahal et al, 2017 ; Ke et al, 2018 ; Lee and Whang, 2020 ). Therefore, these bacterial genera found in all the five rhizospheres of the desert and Antarctic plant species might have synergistic functionalities that help plant with environmental adversities.…”