Chitinophaga tropicalis sp. nov., isolated from forest soil

Abstract

<jats:p>A novel bacterial strain, designated ysch24<jats:sup>T</jats:sup>, was isolated from a forest soil sample collected from the Cat Tien National Park, southern Vietnam. Cells were Gram-stain-negative, aerobic, gliding, filamentous or rod-shaped. The results of 16S rRNA gene analyses revealed that strain ysch24<jats:sup>T</jats:sup> belongs to the genus <jats:italic> <jats:named-content content-type="genus"> <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.8334" xlink:type="simple">Chitinophaga</jats:ext-link> </jats:named-content> </jats:italic>, and was most closely related to <jats:italic> <jats:named-content content-type="species"> <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.34195" xlink:type="simple">Chitinophaga silvisoli</jats:ext-link> </jats:named-content> </jats:italic> GDMCC 1.1411<jats:sup>T</jats:sup> (97.4 %), followed by <jats:italic> <jats:named-content content-type="species"> <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.23620" xlink:type="simple">Chitinophaga oryziterrae</jats:ext-link> </jats:named-content> </jats:italic> JCM 16595<jats:sup>T</jats:sup> (97.3 %) and <jats:italic> <jats:named-content content-type="species"> <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.9783" xlink:type="simple">Chitinophaga sancti</jats:ext-link> </jats:named-content> </jats:italic> NBRC 15057<jats:sup>T</jats:sup> (96.9 %). The average nucleotide identity and digital DNA–DNA hybridization values between strain ysch24<jats:sup>T</jats:sup> and closely related type strains were 72.0–74.0 % and 19.1–19.4 %, respectively. Major fatty acids were iso-C<jats:sub>15 : 0</jats:sub>, C<jats:sub>16 : 1</jats:sub> <jats:italic> </jats:italic>ω5<jats:italic>c</jats:italic> and iso-C<jats:sub>17 : 0</jats:sub> 3-OH and the predominant respiratory quinone was menaquinone 7. Polar lipids consisted of phosphatidylethanolamine, four unidentified aminophospholipids, two unidentified phospholipids and four unidentified lipids. The genomic DNA G+C content was 45.6 mol%. The study clearly showed that strain ysch24<jats:sup>T</jats:sup> should represent a novel species of the genus <jats:italic> <jats:named-content content-type="genus"> <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.8334" xlink:type="simple">Chitinophaga</jats:ext-link> </jats:named-content> </jats:italic>, for which the name <jats:italic>Chitinophaga tropicalis</jats:italic> sp. nov. is proposed. The type strain is ysch24<jats:sup>T</jats:sup> (=GDMCC 1.1355<jats:sup>T</jats:sup>=KACC 21527<jats:sup>T</jats:sup>).</jats:p>

Chitinophaga silvatica sp. nov., isolated from forest soil, and reclassification of Chitinophaga extrema as a later heterotypic synonym of Chitinophaga solisilvae

“…Now, it belongs to the family Chitinophagaceae in the phylum Bacteroidetes while myxobacteria only refer to the group of Myxococcales in Proteobacteria. More than 41 species in the genus Chitinophaga had been validly published, including the recently described Chitinophaga extrema [2], Chitinophaga solisilvae [3], Chitinophaga fulva [4], etc., and most of them were obtained from soil, especially forest soil, such as Chitinophaga tropicalis , Chitinophaga varians , Chitinophaga silvisoli , Chitinophaga caseinilytica , Chitinophaga flava and Chitinophaga dinghuensis [5–10]. This is probably due to their roles in such environments and some Chitinophaga species have shown plant growth promoting (PGP) abilities by altering the carbon source use of their fungal hosts as endosymbiotic bacteria and decomposing both dead fungal and plants in substance recycling [11, 12].…”

Chitinophaga hostae sp. nov., isolated from the rhizosphere soil of Hosta plantaginea

“…The genus Chitinophaga , which is the type genus of the family Chitinophagaceae , was first proposed by Sangkhobol and Skerman with Chitinophaga pinensis as the type species [1]. Until January 2022, the genus Chitinophaga consisted of 43 validly published species (LPSN, https://lpsn.dsmz.de/genus/chitinophaga) [2] including the recently described Chitinophaga fulva [3], Chitinophaga extrema [4], Chitinophaga solisilvae [5], Chitinophaga tropicalis [6] and Chitinophaga vietnamensis [7]. Members of the genus Chitinophaga are Gram-stain-negative, aerobic, rod-shaped or filamentous, and non-spore-forming [8], commonly with phosphatidylethanolamine, unidentified aminolipids and unidentified lipids as the major polar lipids, iso-C 15 : 0 , C 16 : 1 ω 5 c and iso-C 17 : 0 3-OH as the major fatty acids and MK-7 as the major quinone.…”