Chitooligosaccharides enzymatic production by Metarhizium anisopliae

Assis, Cristiane Fernandes de; Araújo, Nathália Kelly de; Pagnoncelli, Maria Giovana Binder; Pedrini, Márcia Regina da Silva; Macêdo, Gorete Ribeiro de; Santos, Everaldo Silvino dos

doi:10.1007/s00449-010-0412-z

Cited by 36 publications

(26 citation statements)

References 39 publications

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“…The reactions were incubated at 55°C for 10, 30 and 40 min. The reaction was interrupted by immersing the tube in boiling water for 10 min (Assis et al 2010). The hydrolysates, denominated here as hydrolysate A, B, and C were analyzed in HPLC.…”

Section: Chitooligosaccharide Productionmentioning

confidence: 99%

Chitooligosaccharides antagonize the cytotoxic effect of glucosamine

Assis

Costa

Melo-Silveira

et al. 2011

World J Microbiol Biotechnol

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Chitooligosaccharides (COS) are partially hydrolyzed compounds derived from chitosan that exhibit a number of biological activities, including antitumor, antibacterial and antifungal properties. In this work, we examined the cytotoxicity of pure COS and oligomers A, B and C (solutions composed of different amounts of COS) produced by enzymatic hydrolysis using a crude enzyme extract produced by the fungus Metarhrizium anisopliae. The antiproliferative effect of these molecules was analyzed using tumor cell lines (HepG2 and HeLa cells) and in a normal cell line (3T3). The antioxidant activity was analyzed in several in vitro experiments. Glucosamine showed higher toxicity (approximately 92%) to all cell lines studied. However, the oligomers obtained after hydrolysis demonstrated no toxic effects on the normal cells (3T3). Furthermore, we showed that a small amount of other COS can decrease the cytotoxic effect of glucosamine against 3T3 cells, indicating that glucosamine could be used as an antitumor drug in the presence of other COS. In addition, different effects were found in antiproliferative assays, which depended on the COS composition in the oligomers (A, B and C), showing that a combination of them may be essential for developing antineoplastic drugs. Superoxide anion scavenging was the main antioxidant activity demonstrated by the COS and oligomers. This activity was also dependent on the oligomer composition of the chitosan hydrolysates. Further work will identify the ideal proportions of COS and glucosamine for maximizing the effects of these biological activities.

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Section: Chitooligosaccharide Productionmentioning

confidence: 99%

Chitooligosaccharides antagonize the cytotoxic effect of glucosamine

Assis

Costa

Melo-Silveira

et al. 2011

World J Microbiol Biotechnol

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“…The monomer (glucosamine) shows the highest concentration. Such a polymer profile suggests the presence of enzymes with exo and endochitosanase activity [12,13]. Figure 2 shows the cytotoxicity of the tested compounds in tumor and normal cells.…”

Section: Resultsmentioning

confidence: 99%

Cytotoxicity of Chitosan Oligomers Produced by Crude Enzyme Extract from the Fungus Metarhizium Anisopliae in Hepg2 and Hela Cells

Assis¹,

Melo-Silveira²,

Oliveira³

2012

J Bioproces Biotechniq

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“…Interestingly, chitosanolytic activity of an entomopathogen, M. anisopliae was used to produce (GlcN) 2-6 from chitosan. 154 Similar to chitinases, some chitosanases also exhibit transglycosylation activity. S. griseus HUT 6037 produced two chitosanases that hydrolyzed partially deacetylated chitin and also carboxymethylcellulose.…”

Section: Applications Of Chitinolytic Enzymesmentioning

confidence: 99%

Chitinolytic enzymes: An appraisal as a product of commercial potential

Chavan

Deshpande

2013

Biotechnology Progress

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Chitin, its deacetylated form, chitosan and chitinolytic enzymes viz. endo-chitinase, N-acetylglucosaminidase, chitosanase, chitin deacetylase (CDA) are gaining importance for their biotechnological applications. Presently, chitin degrading enzymes constitute high-cost low-volume products in human health care and associated research. Indeed chitinases and CDA-chitosanase complex possesss tremendous potential in agriculture to control plant pathogenic fungi and insects. The success in exploring chitinases especially for agriculture, i.e. as a high-volume low-cost product, depends on the availability of highly active preparations with a reasonable cost. Therefore, a reconsideration in terms of understanding the roles of chitinolytic enzymes in applications, e.g. host-pathogen interaction for biocontrol, different mechanisms of chitin degradation, and identification of new enzymes with varying specificities, may make them more useful in a variety of commercial processes in the near future. The possible issues and challenges encountered in the translation of proof of concept into a commercial product will be appraised in this review.

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Chitooligosaccharides enzymatic production by Metarhizium anisopliae

Cited by 36 publications

References 39 publications

Chitooligosaccharides antagonize the cytotoxic effect of glucosamine

Chitooligosaccharides antagonize the cytotoxic effect of glucosamine

Cytotoxicity of Chitosan Oligomers Produced by Crude Enzyme Extract from the Fungus Metarhizium Anisopliae in Hepg2 and Hela Cells

Chitinolytic enzymes: An appraisal as a product of commercial potential

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