Chitosan‐based matrix solid‐phase dispersion extraction assisted cell membrane magnetic bead ligand‐affinity assay for screening active compounds from Fructus Cnidii

Abstract

Cell membrane ligand-affinity assay is a useful tool for screening the active compounds from natural products. However, in traditional cell membrane ligand-affinity assays, natural products need to be refluxed, before being analyzed. This process consumes considerable time and energy and cannot be used for screening natural products that contain thermally unstable compounds. Therefore, an efficient analytical method is required. In this study, chitosan-based matrix solid-phase dispersion extraction was combined with cell membrane magnetic bead ligand-affinity assay to form a novel method for identifying the active compounds in Fructus Cnidii such as osthole and imperatorin. When compared with traditional cell membrane ligand-affinity assays, this assay requires less energy, extraction time (7 min), solvent volume (1.2 mL), and fewer natural products (40 mg). This indicates that the chitosan-based matrix solid-phase dispersion extraction assisted cell membrane magnetic beads ligand-affinity assay is an alternative analytical method for studying natural products.

Miniaturized ternary deep eutectic solvent‐based matrix solid‐phase dispersion: A green sample preparation method for the determination of chlorophenols in river sediment

“…This technique has proven to achieve good results comparable to those of the most advanced and expensive improved procedures despite itssofter extraction conditions. The efficient utilization of innovative sorbents and green extraction solvents is the main breakthrough in MSPD [17][18][19][20]. Ionic liquids (ILs) were utilized in MSPD in place of harmful solvents in this regard [21][22][23][24].…”