Chitosan biopolymer functionalized gold nanoparticles with controlled cytotoxicity and improved antifilarial efficacy

Abstract

Ultra-high stable chitosan functionalized gold nanoparticles (GNPs) of desired biopolymeric corona are synthesized without adding conventional hazardous reducing agents. The inherent unique set of properties like reducing ability, stabilizing effect, and mucoadhesiveness of chitosan is exhibited in the present work. Morphologies and ultra-stability of the synthesized materials are characterized by standard techniques. The mucoadhesiveness of the synthesized materials are well documented through the biological potency of the synthesized GNPs. The prominent bioactivity is evident from the antifilarial activity. The cellular and molecular level studies on the induction of oxidative stress, DNA damage, and undesirable protein expression clearly explain the antifilarial activities. Interestingly, the developed nanoparticle shows no detectable sign of toxicity when evaluated in vitro (rat peritoneal MФ) or in vivo (Wistar rat). Therefore, the synthesized green GNPs appear to be a substantial promise as an efficacious broad-spectrum nanotherapeutic agent with safe outcome for clinical attempt.

Polyphenol enriched ethanolic extract of Cajanus scarabaeoides (L.) Thouars exerts potential antifilarial activity by inducing oxidative stress and programmed cell death

“…The S . cervi , a WHO-recommended model for filarial research, resembles the periodicity and antigenic pattern of the human filarid [15,17–19]. Motile adults of the parasite S .…”

“…In brief, 70 μg of adult S . cervi worm lysate treated with EECs was resolved in 12.5% SDS-PAGE and thereafter electrotransferred to PVDF membrane, and incubated overnight with nematode-specific pro-apoptotic EGL-1, CED-4 antibodies (Santa Cruz Biotechnologies, USA) and anti-apoptotic CED-9 antibody (Santa Cruz Biotechnologies, USA) [5, 15, 19]. Further, the primary antibody probed PVDF membranes were incubated with alkaline phosphatase conjugated secondary IgG (Sigma-Aldrich, MO, USA) for at least 6 h. Finally, the protein expressions were visualized using BCIP/NBT and photographed using chemidoc (Bio-Rad, USA).…”

“…The liver was perfused with PBS, chopped in small pieces (5×5 mm), fixed in Bouin’s fixative and processed for histological preparation following Mukherjee at al. [13] and Chowdhury et al [19]. After histological preparations liver tissue sections were stained with hematoxylin and eosin and observed under brightfield optical microscope (Dewinter, Italy).…”

Surface-Modified Noble Metal Nanoparticles as Antimicrobial Agents: Biochemical, Molecular and Therapeutic Perspectives

“…In this alarming situation, the exploration of noble metal nanoparticles has eventually come out as a boon for all. Bioactive nanoparticle itself or in combination with any antimicrobial drug can provide better intervention, especially against the drug-resistant pathogens (Dey et al 2016;Chowdhury et al 2018Chowdhury et al , 2020. Amongst the metal nanoparticles, silver (Ag), gold (Au) and platinum (Pt) are majorly explored for synthesizing therapeutic nanoparticles.…”

Preparation and Characterization of magnetic PLA/Fe3O4-g-PLLA composite melt blown nonwoven fabric for air filtration

“…[8][9][10] Melt blown nonwoven fabric (MB), a kind of innovative fabric obtained by the one-step melt-blowing process, has drawn people's interest in being used as the high-efficiency air filtration filters or N95-certified mask to treat the serious event of air pollution according to its unique performances. 2,3,[11][12][13][14] Compared with traditional woven fabrics, MB has the simpler manufacturing process, lower prices and better air permeability, meanwhile, still remains the high surface area, small pore size, good barrier and mechanical properties. Thus, MB is regarded as a desired energy saving and consumption reducing replacement for traditional fabric filters.…”