Chitosan-coated magnetic iron oxide nanoparticles for DNA and rhEGF separation

Pérez, Annia Gómez; González‐Martínez, Eduardo; Águila, Carlos R. Díaz; González-Martínez, David A.; Ruíz, Gustavo González; Artalejo, Aymed García; Yee‐Madeira, H.

doi:10.1016/j.colsurfa.2020.124500

Cited by 38 publications

(17 citation statements)

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“…Although MFe 2 O 4 nanoparticles have exhibited excellent characteristics in the biomedical field, their safe application in the human body is a major problem owing to the heavy metal elements presented in MFe 2 O 4 [1], which also limits their applications in the biomedical field. So, as one of the typical nanomaterials, spinel iron oxide nanoparticles without heavy metal elements have been widely applied in many aspects of the biomedicine field due to their good biocompatibility and no toxicity or low toxicity in organisms [2,3], such as magnetic resonance imaging (MRI) [4], clinical diagnosis and the treatment of diseases [5,6], magnetic carriers for drug targeting [7], catalysis [8][9][10][11][12], immune assays [13], targeting photodynamic therapy [14][15][16], cell separation [17], DNA extraction [18], and so on. Therefore, the preparations and applications of iron oxide nanoparticles have attracted more and more researchers [19,20].…”

Section: Introductionmentioning

confidence: 99%

Superparamagnetic α-Fe2O3/Fe3O4 Heterogeneous Nanoparticles with Enhanced Biocompatibility

Wang

Liu

2021

Nanomaterials

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A novel type of magnetic α-Fe2O3/Fe3O4 heterogeneous nanoparticles was prepared via a facile solution combustion process with ferric nitrate and urea as raw materials, and they were characterized by XRD, SEM, TEM, and VSM techniques. The effects of the calcination temperature, the calcination time, the ratio of ferric nitrate and urea, and the heating rate on the relative content of Fe3O4 in the heterogeneous nanoparticles were investigated. The toxicity of α-Fe2O3/Fe3O4 heterogeneous nanoparticles to human hepatocytes L-02, the blood routine, and the histopathological section observation of mice were explored. The results showed that the ratio of ferric nitrate and urea was a key factor to affect the relative content of Fe3O4 in the heterogeneous nanoparticles. The calcination temperature and the calcination time had similar influences, and the corresponding calcination temperature and the calcination time were selected according to their own needs. The CCK8 results initially revealed that α-Fe2O3/Fe3O4 heterogeneous nanoparticles had no effect on cell viability when the concentration of the heterogeneous nanoparticles was less than 100 ng/mL, which suggested their excellent biocompatibility. At the same time, the tail vein administration concentration of 0.9 mg/kg had good biological safety.

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Section: Introductionmentioning

confidence: 99%

Superparamagnetic α-Fe2O3/Fe3O4 Heterogeneous Nanoparticles with Enhanced Biocompatibility

Wang

Liu

2021

Nanomaterials

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“…DNA, proteins, antigens, and antibodies need to be purified from their library before their usage in any biomedical application. [ 221 ] For instance, DNA detection [ 222 ] and separation [ 223 ] is a vital step before the polymerase chain reaction (PCR) step to evaluate specific gene expression. Hei and Cai [ 119 ] reported a method to purify and concentrate RNA of novel coronavirus (SERS‐CoV), which is then amplified PCR to detect the genome sequence of the viral nucleic acid.…”

Section: Biomedical Applications Of Mnpsmentioning

confidence: 99%

Recent progress of magnetic nanoparticles in biomedical applications: A review

et al. 2021

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Magnetic nanoparticles (MNPs) offer tremendous potentialities in biomedical applications for a long while. Since these materials' interactions in biological media largely rely on their crystal structures, sizes, and shapes, detailed studies on their synthesis mechanism for medicinal aspects are crucial. Despite many review reports that have already been published on MNPs, they mainly have focused either on their perspective in biomedical applications or their synthesis and characterization along with functionalization mechanisms as individual entities. For this reason, this review uncovers a comprehensive insight into the ongoing improvement of fabrication processes, surface functionalization of MNPs for biomedical applications together. Besides, various magnetic nanocomposite (MNCs) for smart drug delivery, recent hyperthermia treatment, lab‐on‐a‐chip, and magnetic bio‐separation, and some of the recent emerging imaging techniques using MNPs are discussed. A detailed analysis of toxicity, challenges, and recent progress of clinical trials of MNPs is sketched out to open numerous entryways for advanced research on MNPs for biomedical applications.

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“…Another inorganic nanocarrier, such as carbon, generating noticeable attention about the expansion of improved carrier delivery systems due to the governable topographies such as wider surface area concerning volume ratio, simple surface alteration to upsurge uptake, tuneable size, immunologically inactive surface, and excellent stability in an internal biological setting [ 315 ]. Similarly, iron oxide is a very prevalent examined material for inorganic nanocarrier production, and the majority of them are FDA-permitted nanomedicines or vehicles for gene delivery [ 269 , 316 , 317 ]. Also, magnetic iron-based gene-editing tools are compounds of maghemite (Fe 2 O 3 ) or magnetite (Fe 3 O 4 ) that retain superparamagnetic characteristics with confined structures or sizes and have shown triumph as gene delivery systems and contrast agents in thermal-based cancer therapy [ 292 ].…”

Section: Delivery Vehicles For Crispr/cas-based Targeting In Tnbcmentioning

confidence: 99%