Chitosan-coated magnetic nanoparticles as carriers of 5-Fluorouracil: Preparation, characterization and cytotoxicity studies

Zhu, Li; Ma, Jingwei; Jia, Nengqin; Zhao, Yu; Shen, Hujun

doi:10.1016/j.colsurfb.2008.07.020

Cited by 208 publications

(108 citation statements)

References 29 publications

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“…Nevertheless, the 5-FU nanospheres inhibited PC-3 proliferation to a similar extent, and with a similar kinetics to that displayed on HT29. By contrast, blank chitosan nanospheres were completely ineffective in all the experiments (data not shown), as previously reported for different chitosan formulation [42].The nanosphere formulation may increase the amount of 5-FU entering the two cell lines. Indeed fluorescent chitosan nanopheres can be internalized by cancer cells (data not shown).…”

Section: Resultssupporting

confidence: 87%

New Chitosan Nanospheres for the Delivery of 5-Fluorouracil: Preparation, Characterization and in vitro Studies

Cavalli¹,

Leone²,

Minelli³

et al. 2014

CDD

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The aim of this work is to develop new chitosan nanospheres for the delivery of 5-fluorouracil (5-FU). Drug loaded nanospheres were prepared using a technique derived from a combination of coacervation and emulsion droplet coalescence methods. The size and morphology of nanospheres were characterized by laser light scattering and transmission electron microscopy. The 5-FU interaction with chitosan nanospheres was investigated by DSC analysis and FT-IR spectroscopy. The in vitro release was studied by dialysis bag technique. Cytotoxicity of 5-FU loaded chitosan nanospheres was evaluated in vitro on HT29 and PC-3 cell lines. The effects of 5-FU loaded chitosan nanospheres on adhesion of tumor cells to human umbilical vein endothelial cells (HUVEC) were also investigated. 5-FU loaded chitosan nanospheres appeared with a spherical shape, with a mean diameter of about 200 nm and a negative zeta potential of about -6.0 mV. The successful interaction between drug and chitosan nanosphere matrix was demonstrated by both DSC and FT-IR analyses. The quantitative determination of 5-FU was assayed by UV-Vis analysis. The encapsulation efficiency of 5-FU content was about 70%. A kinetic study of in vitro release demonstrated that the percentages of 5-FU delivered from nanospheres was approx. 10% after 3 hours. The in vitro studies showed that 5-FU loaded nanospheres were effective in reducing tumor cell proliferation in a time-and concentration-dependent manner. 5-FU nanospheres were also able to inhibit both HT29 and PC-3 adhesion to HUVEC after 48 hours of treatment.

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Section: Resultssupporting

confidence: 87%

New Chitosan Nanospheres for the Delivery of 5-Fluorouracil: Preparation, Characterization and in vitro Studies

Cavalli¹,

Leone²,

Minelli³

et al. 2014

CDD

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“…The release of nanoparticles in SBF occurred in stages, characterized by sustained release, which was similar to previous studies. 30 Rapid release was observed between 0 and 6 hours, with a cumulative release of 20.6%, resulting from diffusion of surface 5-FU into the SBF solution. Smooth slow-release occurred after between 6 hours and 7 days.…”

mentioning

confidence: 99%

Optimized synthesis of glycyrrhetinic acid-modified chitosan 5-fluorouracil nanoparticles and their characteristics

Cheng¹,

Chen²,

Wang³

et al. 2014

IJN

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The nanoparticle drug delivery system, which uses natural or synthetic polymeric material as a carrier to deliver drugs to targeted tissues, has a broad prospect for clinical application for its targeting, slow-release, and biodegradable properties. Here, we used chitosan (CTS) and hepatoma cell-specific binding molecule glycyrrhetinic acid to synthesize glycyrrhetinic acid-modified chitosan (GA-CTS). The synthetic product was confirmed by infrared (IR) spectra and hydrogen-1 nuclear magnetic resonance. The GA-CTS/5-fluorouracil (5-FU) nanoparticles were synthesized by combining GA-CTS and 5-FU and conjugating 5-FU onto the GA-CTS nanomaterial. The central composite design was performed to optimize the preparation process as CTS:tripolyphosphate sodium (TPP) weight ratio =5:1, 5-FU:CTS weight ratio =1:1, TPP concentration =0.05% (w/v), and cross-link time =50 minutes. GA-CTS/5-FU nanoparticles had a mean particle size of 193.7 nm, a polydispersity index of 0.003, a zeta potential of +27.4 mV, and a drug loading of 1.56%. The GA-CTS/5-FU nanoparticle had a protective effect on the drug against plasma degrading enzyme, and provided a sustained release system comprising three distinct phases of quick, steady, and slow release. Our study showed that the peak time, half-life time, mean residence time and area under the curve of GA-CTS/5-FU were longer or more than those of the 5-FU group, but the maximum concentration (C max ) was lower. We demonstrated that the nanoparticles accumulated in the liver and have significantly inhibited tumor growth in an orthotropic liver cancer mouse model.

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“…Thus, it is a nontoxic biocompatible amino polysaccharide that has a number of medical applications in drug release in aqueous solutions at pH < 6.5. Specifically, chitosan oligosaccharide has been applied to a variety of magnetic nanoparticles in order to enhance their colloidal stability and circulation (Zhu et al, 2009;Bae et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Chitosan-Coated Fe<sub>3</sub>O<sub>4</sub> Magnetic Nanoparticles as Carrier of Cisplatin for Drug Delivery

Arum¹,

Oh²,

Kang³

et al. 2015

Fisheries and aquatic sciences

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A synthesis method for a chitosan-coated magnetic drug-delivery system of cisplatin is proposed. Here, cisplatin was conjugated to the surface of Magnetite (Fe 3 O 4 ) nanoparticles via a (3-Aminopropyl)-trimethoxysilane (APTS) coupling agent. To reduce the cytotoxic effect of cisplatin, the magnetic drug was then encapsulated in chitosan (CS-cisplatin-Fe 3 O 4 ) through the water/oil (W/O) emulsion method. The CS-cisplatin-Fe 3 O 4 nanoparticles were synthesized in a spherical shape with a diameter of 190 nm. The cytotoxicity assay was performed using HeLa cells. The cisplatin uptake of the cells was determined using High Performance Liquid Chromatography (HPLC) to calculate the drug content. The controlled release of cisplatin was demonstrated by regulating the dissolution and diffusion of the drug through the chitosan matrix.

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Chitosan-coated magnetic nanoparticles as carriers of 5-Fluorouracil: Preparation, characterization and cytotoxicity studies

Cited by 208 publications

References 29 publications

New Chitosan Nanospheres for the Delivery of 5-Fluorouracil: Preparation, Characterization and in vitro Studies

New Chitosan Nanospheres for the Delivery of 5-Fluorouracil: Preparation, Characterization and in vitro Studies

Optimized synthesis of glycyrrhetinic acid-modified chitosan 5-fluorouracil nanoparticles and their characteristics

Chitosan-Coated Fe<sub>3</sub>O<sub>4</sub> Magnetic Nanoparticles as Carrier of Cisplatin for Drug Delivery

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