Chitosan Cross-Linked Pentasodium Tripolyphosphate Micro/Nanoparticles Produced by Ionotropic Gelation

Severino, Patrícia; Silva, Classius Ferreira da; Silva, Mariana A. da; Santana, Maria Helena Andrade; Souto, Eliana B.

doi:10.1007/s12355-014-0360-z

Cited by 35 publications

(25 citation statements)

References 27 publications

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“…Nanoparticles were produced by an ionic crosslinking technique [10], using sodium tripolyphosphate (TPP) as the crosslinking agent, in 30% ( w / w ) concentration ratio of TPP to total chitosan amount, and mechanical stirring at approximately 350 rpm (Multistirrer 15 Magnetic Stirrer, Velp Scientific Inc., Bohemia, NY, USA). In general, for each 4 mL of chitosan solution at 2.5 mg/mL (in acetic acid 1%, v / v , pH 5.0, previously filtered through 0.45 μm Millipore™ membranes), 6 mL of TPP solution at 0.5 mg/mL (filtered through 0.22 μm) was added dropwise (30 s).…”

Section: Methodsmentioning

confidence: 99%

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Effect of Polysaccharide Sources on the Physicochemical Properties of Bromelain–Chitosan Nanoparticles

et al. 2019

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Bromelain, a set of proteolytic enzymes potential pharmaceutical applications, was encapsulated in chitosan nanoparticles to enhance enzyme stability, and the effect of different chitosan sources was evaluated. Chitosan types (i.e., low molecular weight chitosan, chitosan oligosaccharide lactate, and chitosan from shrimp shells) produced nanoparticles with different physicochemical properties, however in all cases, particle size and zeta potential decreased, and polydispersity index increased after bromelain addition. Bromelain encapsulation was higher than 84% and 79% for protein content and enzymatic activity, respectively, with low molecular weight chitosan presenting the highest encapsulation efficiency. Nanoparticle suspension was also tested for accelerated stability and rheological behavior. For the chitosan–bromelain nanoparticles, an instability index below 0.3 was recorded and, in general, the loading of bromelain in chitosan nanoparticles decreased the cohesiveness of the final suspension.

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Section: Methodsmentioning

confidence: 99%

“…Chitosan is obtained from the deacetylation of chitin, which is derived from crustacean shells and fungi cell walls, being one of the most abundant natural polysaccharides [2,9,10,11]. Reaction conditions, extent, and chitin source are factors that can be modified during the chitin deacetylation process.…”

Section: Introductionmentioning

confidence: 99%

Effect of Polysaccharide Sources on the Physicochemical Properties of Bromelain–Chitosan Nanoparticles

et al. 2019

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“…This technique can also be used to count particles, which is a feature not present in DLS . SEM is employed to verify sizes and shape in the case of microparticles …”

Section: Characterizing the Nanoparticles/microparticlesmentioning

confidence: 99%

Ionotropic gelation method in the synthesis of nanoparticles/microparticles for biomedical purposes

Pedroso‐Santana

Fleitas‐Salazar

2020

Polymer International

167

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The encapsulation of drugs inside polymeric nanoparticles/microparticles is a strategy currently employed in the search for new and more effective therapies. The use of biocompatible and biodegradable polymers gives several advantages to these formulations. Protection of the active principals against the action of environmental and physiological agents, the reduced number of doses and a subsequent decrease in drug-related adverse effects, and increased bioavailability are some of these advantages. Several methods and materials are now used to synthesize nanoparticles/microparticles for biomedical applications, from carbon-derived structures to metallic and lipid particles. However, among the methods using polymers, ionotropic gelation is one of the more affordable and easier procedures to perform in daily laboratory work. In this mini-review, we address relevant characteristics of ionotropic gelation, beginning with basic aspects of the technique, which reagents and conditions are commonly used, the factors affecting the stability of the formulation, the advantages and disadvantages of the method and some principal characterization techniques for the nanoparticulate/microparticulate formulation. Finally, we conclude with a few important considerations.

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“…e procedure followed a previously reported methodology [18,19]. Briefly, a CS solution in acetic acid 1% w/v was used as a cationic agent, instead of CaCl 2 .…”

Section: Polyelectrolyte Complexationmentioning

confidence: 99%

Polyelectrolyte Complexation versus Ionotropic Gelation for Chitosan-Based Hydrogels with Carboxymethylcellulose, Carboxymethyl Starch, and Alginic Acid

Henao

Delgado

Contreras

et al. 2018

International Journal of Chemical Engineering

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e preparation of gels by charge interaction methods has been extensively studied, but it is not yet clear how these methods influence gel characteristics. e objective of this work was to study differences in morphology and surface charge of hydrogels prepared by ionotropic gelation, polyelectrolyte complexation, and a combination of both methods. us, the anionic charge was provided by carboxymethylcellulose (CMC), carboxymethylated starch (CMS), and alginic acid (AA); calcium chloride (CaCl 2 ) and chitosan (CS) were used for the ionotropic gelation and polyelectrolyte complexation, respectively. ose materials are commercially available, have low toxicity, and are widely used in the area. ese compounds interact through physical crosslinks, which are affected by physical changes of the medium. Our results showed that these two methods produced changes in the morphology of the hydrogels. CMC gels exhibited larger pores in the presence of CaCl 2 . In polyelectrolyte complexation, CMS produced an increased agglomeration of particles, while the addition of CaCl 2 to AA generated dispersed particles of size in the order of millimeters. Mixing both ionotropic gelation and polyelectrolyte complexation methods yielded gels of varied charge (568 mV for CMC, 502 mV for CMS, and 1713 mV for AA). FTIR spectra of the hydrogels showed interactions between the different polymeric compounds, being the greatest changes between 1250 and 1600 cm −1 , due possibly to the replacement of Na by Ca at crosslinking points. erefore, the method of gel preparation employed had a major influence on the size and pore distribution, parameters which in turn influence encapsulation and drug delivery in these systems.

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Chitosan Cross-Linked Pentasodium Tripolyphosphate Micro/Nanoparticles Produced by Ionotropic Gelation

Cited by 35 publications

References 27 publications

Effect of Polysaccharide Sources on the Physicochemical Properties of Bromelain–Chitosan Nanoparticles

Effect of Polysaccharide Sources on the Physicochemical Properties of Bromelain–Chitosan Nanoparticles

Ionotropic gelation method in the synthesis of nanoparticles/microparticles for biomedical purposes

Polyelectrolyte Complexation versus Ionotropic Gelation for Chitosan-Based Hydrogels with Carboxymethylcellulose, Carboxymethyl Starch, and Alginic Acid

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