Chitosan-DNA nanoparticles enhanced the immunogenicity of multivalent DNA vaccination on mice against Trueperella pyogenes infection

Huang, Ting; Song, Xuhao; Jie, Jing; Zhao, Kelei; Shen, Yongmei; Zhang, Xiuyue; Yue, Bisong

doi:10.1186/s12951-018-0337-2

Cited by 57 publications

(50 citation statements)

References 48 publications

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“…To prevent bronchitis caused by respiratory syncytial virus (RSV), chitosan-DNA nanospheres administered intranasally induced the expression of corresponding protein, and improved the levels of anti-RSVspecific IgG, sIgA, cytotoxic T lymphocytes, and IFN- [62]. The chitosan-DNA nanoparticles were effective against diseases of the T. pyogenes infection and provided strategies for further development of novel vaccines encapsulated in chitosan nanoparticles [63].…”

Section: Chitosan-based Nanoparticlesmentioning

confidence: 99%

Applications of polymer-based nanoparticles in vaccine field

Guo

Utupova

et al. 2019

Nanotechnology Reviews

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Polymer-based nanoparticles have good solubility, stability, safety, and sustained release,which increases the absorption of loaded drugs, protects the drugs from degradation, and prolongs their circulation time and targeted delivery. Generally, we believe that prevention and control of infectious diseases through inoculation is the most efficient measure. However, these vaccines including live attenuated vaccines, inactivated vaccines, protein subunit vaccines, recombinant subunit vaccines, synthetic peptide vaccines and DNA vaccines have several defects, such as immune tolerance, poor immunogenicity, low expression level and induction of respiration pathological changes. All kinds of biodegradable natural and synthetic polymers play major roles in the vaccine delivery system to control the release of antigens for an extended period of time. In addition, these polymers also serve as adjuvants to enhance the immunogenicity of vaccine. This review mainly introduces natural and synthetic polymer-based nanoparticles and their formulation and properties. Moreover, polymer-based nanoparticles as adjuvants and delivery carriers in the applications of vaccine are also discussed. This review provides the basis for further operation of nano vaccines by utilizing the polymer-based nanoparticles as vaccine adjuvants and delivery systems. Polymer-based nanoparticles have exhibited great potential in improving the immunogenicity of antigens and the development of nano vaccines in future.

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Section: Chitosan-based Nanoparticlesmentioning

confidence: 99%

Applications of polymer-based nanoparticles in vaccine field

Guo

Utupova

et al. 2019

Nanotechnology Reviews

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“…Helicobacter pylori, a causative agent of gastritis, duodenal ulcers, gastric ulcer and cancer [29], is an opportunistic and infectious bacterium not only in humans, but also in animals. The infection rates are over 50.8% in developing countries whereas they are more than 34.7% in developed countries [30].…”

Section: Discussionmentioning

confidence: 99%

“…Intramuscular immunization is very effective in eliciting humoral and cellular immune responses. Previous researches have proved that virulence genes enhance the immunity of the vaccine, and chitosan provides longer residence times by intramuscular infection [29,42], allowing the vaccine to access the lymphoid tissue better and resulting in increased IgG production. Therefore, our study further confirmed the stimulation effect of humoral immune responses of cagW and chitosan in mice in comparison to the background signal stimulated by pcDNA3.1 (+) and PBS.…”

Section: Discussionmentioning

confidence: 99%

Effect of the cagW-based gene vaccine on the immunologic properties of BALB/c mouse: an efficient candidate for Helicobacter pylori DNA vaccine

Chehelgerdi

Doosti

2020

J Nanobiotechnol

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Background: Helicobacter pylori (H. pylori) infect more than half of the world population, and they cause different serious diseases such as gastric carcinomas. This study aims to design a vaccine on the basis of cagW against H. pylori infection. After pcDNA3.1 (+)-cagW-CS-NPs complex is produced, it will be administered into the muscles of healthy BALB/c mice in order to study the effect of this DNA vaccine on the interleukin status of mice, representing its effect on the immune system. After that, the results will be compared with the control groups comprising the administration of cagW-pCDNA3.1 (+) vaccine, the administration of chitosan and the administration of PBS in the muscles of mice. Methods: The cagW gene of H. pylori was amplified by employing PCR, whose product was then cloned into the pcDNA3.1 (+) vector, and this cloning was confirmed by PCR and BamHI/EcoRV restriction enzyme digestion. CagW gene DNA vaccine was encapsulated in chitosan nanoparticles (pcDNA3.1 (+)-cagW-CS-NPs) using a complex coacervation method. The stability and in vitro expression of chitosan nanoparticles were studied by DNase I digestion and transfection, and the immune responses elicited in specific pathogen-free (SPF) mice by the pcDNA3.1 (+)-cagW-CS-NPs were evaluated. Apart from that, the protective potential pcDNA3.1 (+)-cagW-CS-NPs was evaluated by challenging with H. pylori. Results: The pcDNA3.1 (+)-cagW-CS-NPs comprises cagW gene of H. pylori that is encapsulated in chitosan nanoparticles, produced with good morphology, high stability, a mean diameter of 117.7 nm, and a zeta potential of + 5.64 mV. Moreover, it was confirmed that chitosan encapsulation protects the DNA plasmid from DNase I digestion, and the immunofluorescence assay showed that the cagW gene could express in HDF cells and maintain good bioactivity at the same time. In comparison to the mice immunized with the control plasmid, in vivo immunization revealed that mice immunized with pcDNA3.1 (+)-cagW-NPs showed better immune responses and prolonged release of the plasmid DNA. Conclusions: This research proves chitosan-DNA nanoparticles as potent immunization candidates against H. pylori infection and paves the way for further developments in novel vaccines encapsulated in chitosan nanoparticles.

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Section: Discussionmentioning

confidence: 99%

Effect of the cagW-based gene vaccine on the immunologic properties of BALB/c mouse: an efficient candidate for Helicobacter pylori DNA vaccine

Chehelgerdi

Doosti

2020

Preprint

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Background Helicobacter pylori ( H. pylori ) infect more than half of the world population, and they cause different serious diseases such as gastric carcinomas. This study aims to design a vaccine on the basis of cagW against H. pylori infection. After pcDNA3.1 (+)- cagW -CS-NPs complex is produced, it will be administered into the muscles of healthy BALB/c mice in order to study the effect of this DNA vaccine on the interleukin status of mice, representing its effect on the immune system. After that, the results will be compared with the control groups comprising the administration of cagW -pCDNA3.1 (+) vaccine, the administration of chitosan and the administration of PBS in the muscles of mice. Methods The cagW gene of H. pylori was amplified by employing PCR, whose product was then cloned into the pcDNA3.1 (+) vector, and this cloning was confirmed by PCR and BamHI/EcoRV restriction enzyme digestion. CagW gene DNA vaccine was encapsulated in chitosan nanoparticles (pcDNA3.1 (+)- cagW -CS-NPs) using a complex co-acervation method. The stability and in vitro expression of chitosan nanoparticles were studied by DNase I digestion and transfection, and the immune responses elicited in specific pathogen-free (SPF) mice by the pcDNA3.1 (+)-cagW-CS-NPs were evaluated. Apart from that, the protective potential pcDNA3.1 (+)- cagW -CS-NPs was evaluated by challenging with H.pylori . Results The pcDNA3.1 (+)- cagW -CS-NPs comprises cagW gene of H. pylori that is encapsulated in chitosan nanoparticles, produced with good morphology, high stability, a mean diameter of 117.7 nm, and a zeta potential of + 5.64 mV. Moreover, it was confirmed that chitosan encapsulation protects the DNA plasmid from DNase I digestion, and the immunofluorescence assay showed that the cagW gene could express in HDF cells and maintain good bioactivity at the same time. In comparison to the mice immunized with the control plasmid, in vivo immunization revealed that mice immunized with pcDNA3.1 (+)- cagW -NPs showed better immune responses and prolonged release of the plasmid DNA. Conclusions This research proves chitosan-DNA nanoparticles as potent immunization candidates against H. pylori infection and paves the way for further developments in novel vaccines encapsulated in chitosan nanoparticles.

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Chitosan-DNA nanoparticles enhanced the immunogenicity of multivalent DNA vaccination on mice against Trueperella pyogenes infection

Cited by 57 publications

References 48 publications

Applications of polymer-based nanoparticles in vaccine field

Applications of polymer-based nanoparticles in vaccine field

Effect of the cagW-based gene vaccine on the immunologic properties of BALB/c mouse: an efficient candidate for Helicobacter pylori DNA vaccine

Effect of the cagW-based gene vaccine on the immunologic properties of BALB/c mouse: an efficient candidate for Helicobacter pylori DNA vaccine

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