Chitosan encapsulation modulates the effect of trans-cinnamaldehyde on AHL-regulated quorum sensing activity

Qin, Xiaofei; Kräft, T.; Goycoolea, Francisco M.

doi:10.1016/j.colsurfb.2018.05.054

Cited by 20 publications

(15 citation statements)

References 57 publications

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“…Given that the QS system operates with AHL concentrations orders of magnitude lower than those tested for chitosan in the antimicrobial assays, the concentrations of chitosan tested in this assay were 160- to 4-fold lower than those tested in the antibacterial assays. The bacterial strain is the same one that we have used in parallel studies [ 26 , 27 , 28 , 29 , 30 , 33 ]. It is a green fluorescent protein (GFP) reporter that does not synthesize its own AHL, namely 3OC 6 HSL, and responds by producing GFP upon exogenous addition of extremely low concentrations of 3OC 6 HSL (<1 nM).…”

Section: Resultsmentioning

confidence: 99%

“…In the present work, complementary to the two parallel ones, we have investigated the antibacterial activity of 10 chitosan samples of varying M w and DA. This enabled us to select one non-toxic chitosan to explore in further detail its anti-QS activity using the Escherichia coli Top10 reporter strain that we have used in previous works [ 26 , 28 , 29 , 30 ].…”

Section: Introductionmentioning

confidence: 99%

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Assessment of the Quorum Sensing Inhibition Activity of a Non-Toxic Chitosan in an N-Acyl Homoserine Lactone (AHL)-Based Escherichia coli Biosensor

2018

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New approaches to deal with drug-resistant pathogenic bacteria are urgent. We studied the antibacterial effect of chitosans against an Escherichia coli quorum sensing biosensor reporter strain and selected a non-toxic chitosan to evaluate its quorum sensing (QS) inhibition activity and its effect on bacterial aggregation. To this end, chitosans of varying degree of acetylation (DA) (12 to 69%) and molecular weight (Mw) (29 to 288 kDa) were studied. Only chitosans of low DA (~12%) inhibited bacterial growth, regardless of their Mw. A chitosan with medium degree of polymerization (named MDP) DA30, with experimental DA 42% and Mw 115 kDa was selected for further QS inhibition and scanning electron microscopy (SEM) imaging studies. MDP DA30 chitosan exhibited QS inhibition activity in an inverse dose-dependent manner (≤12.5 µg/mL). SEM images revealed that this chitosan, when added at low concentration (≤30.6 µg/mL), induced substantial bacterial aggregation, whereas at high concentration (234.3 µg/mL), it did not. Aggregation explains the QS inhibition activity as the consequence of retardation of the diffusion of N-acylated homoserine lactones (AHLs).

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Assessment of the Quorum Sensing Inhibition Activity of a Non-Toxic Chitosan in an N-Acyl Homoserine Lactone (AHL)-Based Escherichia coli Biosensor

2018

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“…Due to the increased antibiotic resistance among the human bacterial pathogens and the current poor effects of antibiotics to treat bacterial infections, finding novel alternative antimicrobial approaches is urgently needed. [5][6][7]33 Correspondingly, one of these approaches is targeting the expression of the QSregulated pathogenic factors using the analogs of the QS molecules. In this regard, they have been developed as Figure 1 The LuxR/AHL-mediated quorum sensing the regulation of the target genes' expressions in P. aeruginosa.…”

Section: Quorum Quenchingmentioning

confidence: 99%

“…Therefore, the chitosan NPs with vast surface areas modifies the bacterial membrane penetrability via the membrane incorporation and also leads to the death of bacteria. 33,[97][98][99] Furthermore, chitosan NPs have shown anti-QS properties by disrupting biofilm production, decreasing the expression of lasR and rhlR genes, and reducing the secretion of pyocyanin and proteases in P. aeruginosa. 100 In addition, chitosan NPs have a negative effect on the pathogenic factors produced by P. aeruginosa PAO1.…”

Section: Synthetic Compoundsmentioning

confidence: 99%

<p>Quorum Quenching: A Potential Target for Antipseudomonal Therapy</p>

Hemmati¹,

Salehi²,

Ghotaslou³

et al. 2020

IDR

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There has been excessive rate of use of antibiotics to fight Pseudomonas aeruginosa (P. aeruginosa) infections worldwide, which has consequently caused the increased resistance to multiple antibiotics in this pathogen. Due to the widespread resistance and the current poor effect of antibiotics consumed to treat P. aeruginosa infections, finding some novel alternative therapeutic methods are necessary for the treatment of infections. The P. aeruginosa biofilms can cause severe infections leading to the increased antibiotic resistance and mortality rate among the patients. In this regard, there are no approaches that can efficiently manage these infections; therefore, novel and effective antimicrobial and antibiofilm agents are needed to control and treat these bacterial infections. Quorum sensing inhibitors (QSIs) or quorum quenchings (QQs) are now considered as potential therapeutic alternatives and/or adjuvants to the current failing antibiotics, which can control the virulence traits of the pathogens, so as a result, the host immune system can quickly eliminate bacteria. Thus, the aims of this review article were presenting a brief explanation of the research reports on the natural and synthetic QSIs of P. aeruginosa, and the assessment of the current understanding on the QS mechanisms and various QQ strategies in P. aeruginosa.

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“…In the present work, complementary to the two parallel ones, we have addressed the antibacterial activity of ten samples of chitosans of varying MW and DA. This enabled us to select one non-toxic chitosan to explore in further detail its anti-QS activity using the E. coli Top 10 reporter strain that we have used in previous works [26,[35][36][37].…”

Section: Introductionmentioning

confidence: 99%

Assessment of the Quorum Sensing Inhibition Activity of a Non-Toxic Chitosan in a AHL-Based <em>E. coli</em> Biosensor

Xin¹,

Emich²,

Goycoolea³

2018

Preprint

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New approaches to deal with drug-resistant pathogenic bacteria are urgent. We studied the antibacterial effect of chitosans against an E. coli quorum sensing biosensor reporter strain, and selected a non-toxic chitosan to evaluate its QS inhibition activity and its effect on bacterial aggregation. To this end, chitosans of varying DA (12 to 69%) and Mw (29 to 288 KDa) were studied. Only chitosans of low DA (~12%) inhibited the bacterial growth, regardless of the Mw. Chitosan MDP DA30 (DA 42% and Mw 115 kDa) was selected for further QS inhibition and SEM imaging studies. MDP DA30 chitosan exhibited QS inhibition activity in an inverse dose-dependent manner (≤12.5 µg/mL). SEM images revealed that this chitosan, when added at low concentration (≤30.6 µg/mL), induced substantial bacterial aggregation, whereas at high concentration (234.3 µg/mL), it did not. Aggregation explains the QS inhibition activity as the consequence of retardation of the diffusion of AHL.

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Chitosan encapsulation modulates the effect of trans-cinnamaldehyde on AHL-regulated quorum sensing activity

Abstract: This is a repository copy of Chitosan encapsulation modulates the effect of trans-cinnamaldehyde on AHL-regulated quorum sensing activity.

Cited by 20 publications

References 57 publications

Assessment of the Quorum Sensing Inhibition Activity of a Non-Toxic Chitosan in an N-Acyl Homoserine Lactone (AHL)-Based Escherichia coli Biosensor

Assessment of the Quorum Sensing Inhibition Activity of a Non-Toxic Chitosan in an N-Acyl Homoserine Lactone (AHL)-Based Escherichia coli Biosensor

<p>Quorum Quenching: A Potential Target for Antipseudomonal Therapy</p>

Assessment of the Quorum Sensing Inhibition Activity of a Non-Toxic Chitosan in a AHL-Based <em>E. coli</em> Biosensor

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