Chitosan-g-polyaniline: a creatine amidinohydrolase immobilization matrix for creatine biosensor

Abstract

Abstract.A novel matrix composed of chitosan-graft-polyaniline (CHIT-g-PANI) was electrochemically prepared to investigate the immobilization of creatine amidinohydrolase (CAH). CAH enzyme was covalently immobilized with the CHIT-g-PANI matrix using glutaraldehyde as a linker. The resulting CAH/CHIT-g-PANI biomatrix was characterized with Fourier transform infrared spectroscopy (FTIR), atomic force microscopy (AFM), contact angle measurement and cyclic voltammetry (CV) taking CHIT-g-PANI as a reference. The influence of various parameters on CAH enzyme activity within the matrix was investigated including pH, temperature, and time. The Michaelis-Menten constant and apparent activities for the CAH enzyme were calculated to be 0.51 mM and 83.59 mg/cm 2 , respectively; indicating CHIT-g-PANI matrix has a high affinity to immobilize CAH enzyme.

Gum ghatti based novel electrically conductive biomaterials: A study of conductivity and surface morphology

“…Conducting form of PANI is described by band at 1237 cm -1 [10,46]. The bands at 796 and 1731 cm -1 were corresponding to an out-of-plane bending vibration of C-H on para-disubstituted rings and carboxyl groups, respectively [10,47,48]. It has been reported that the band at 1110 cm -1 to be a measure of the degree of the delocalization of electrons and is also revealing of high conductivity.…”

Zein-Polyaniline Blends—a Route to Electrically Conductive Biopolymer

“…5C, component 2) can appear due to hydrogen bonding or covalent bonding between zein and PANI, in particular NH 2 and OH groups in zein molecules. These types of interactions were reported for chitosan [19,20] and polysaccharide [10] in their combination with PANI. The N(1s) peaks were also seen in all the samples.…”

“…The second way was attempted with the aim to study some possible interactions between zein and PANI reported already for some other products containing PANI prepared by in situ oxidative polymerisation, e.g. polysaccharide-PANI Downloaded by [University of Tasmania] at 21:17 13 October 2014 [10] and chitosan-PANI [19,20] because such an order of mixing ingredients is more favorable for a grafting polymerisation rather than formation of homopolymer.…”

Current Frontiers in Electrochemical Biosensors Using Chitosan Nanocomposites

“…The effect of pH, temperature, and time on the CAH enzyme activity within the matrix was studied. The Michaelis -Menten constant and apparent activity for the CAH enzyme were calculated to be 0.51 mM and 83.59 mg cm − 2 , which was attributed to the fact that the chitosan -graft -PANI matrix had a high affi nity to immobilize CAH enzyme [30] . MWNTs, Pt -NPs, and a sol -gel of chitosan -silica organic -inorganic hybrid composite was used to develop a glucose biosensor.…”