Chitosan-Gelatin Films Cross-Linked with Dialdehyde Cellulose Nanocrystals as Potential Materials for Wound Dressings

Abstract

In this study, thin chitosan-gelatin biofilms cross-linked with dialdehyde cellulose nanocrystals for dressing materials were received. Two types of dialdehyde cellulose nanocrystals from fiber (DNCL) and microcrystalline cellulose (DAMC) were obtained by periodate oxidation. An ATR-FTIR analysis confirmed the selective oxidation of cellulose nanocrystals with the creation of a carbonyl group at 1724 cm−1. A higher degree of cross-linking was obtained in chitosan-gelatin biofilms with DNCL than with DAMC. An increasing amount of added cross-linkers resulted in a decrease in the apparent density value. The chitosan-gelatin biofilms cross-linked with DNCL exhibited a higher value of roughness parameters and antioxidant activity compared with materials cross-linked with DAMC. The cross-linking process improved the oxygen permeability and anti-inflammatory properties of both measurement series. Two samples cross-linked with DNCL achieved an ideal water vapor transition rate for wound dressings, CS-Gel with 10% and 15% addition of DNCL—8.60 and 9.60 mg/cm2/h, respectively. The swelling ability and interaction with human serum albumin (HSA) were improved for biofilms cross-linked with DAMC and DNCL. Significantly, the films cross-linked with DAMC were characterized by lower toxicity. These results confirmed that chitosan-gelatin biofilms cross-linked with DNCL and DAMC had improved properties for possible use in wound dressings.

Development of functional active films from blend of gelatin with crude orange juice pomace pectin: Test for packaging of virgin olive oil

“…A general trend was observed, showing that an increase in the ratio of SOPP from 0% to 50% (w/w) within the gelatin film matrix led to a significant increase in the apparent density from 1.08 ± 0.02 in CFG (100/0) to 1.5 ± 0.08 in the G‐SOPP (50/50). These results were found to be similar, as already presented in the literature for gelatin‐based films incorporated with polysaccharide hydrocolloids 78,79 . This variation in apparent density can be attributed to the changes in solid content density and porosity of films by adding SOPP 80 .…”

Schiff base crosslinked gelatin–Spirulina platensis protein concentrate films with enhanced antioxidant activity

“…The suspension (film + supernatant) was then mixed with 5 mL of 0.06 mmol/L DPPH stock solution and allowed to react in the dark at ambient temperature for 30 min. The absorbance of the supernatant was measured at 517 nm and RSA% was calculated as described for PC (Wegrzynowska‐Drzymalska et al., 2022). All determinations were performed in duplicate.…”

A Review on Chitosan and Cellulose Hydrogels for Wound Dressings

“…Cellulose nanocrystals are excellent biomaterials with tunable surface chemistry. Recently, several studies have been focused on the topic of modification of cellulose nanocrystals, such as by esterification, oxidation [ 43 ], carbamation, amidation, etherification [ 44 ]. In the past years, it has been reported that cellulose nanocrystals can be oxidized with periodate and form several aldehyde groups [ 45 ].…”

“…Therefore, dialdehyde cellulose nanocrystals may react with the free amino groups from chitosan or gelatin same as glutaraldehyde. This type of reaction is widely known as the Schiff base reaction [ 44 ].…”