Chitosan/gelatin/PVA membranes for mammalian cell culture

Mhatre, Aditi; Bhagwat, Advait; Bangde, Prachi; Jain, Ratnesh; Dandekar, Prajakta

doi:10.1016/j.carpta.2021.100163

Cited by 11 publications

(15 citation statements)

References 43 publications

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“…[ 19 ] The shifting of absorption peaks at ≈3420, ≈1650, and ≈1560 cm −1 from the FTIR spectra of CG1:3/P, CG1:1/P, and CG31/P nanofibers indicated the formation of hydrogen bonding between NH and OH groups of chitosan, NH, OH, and CO groups of gelatin, and OH group of PVA. [ 14,19,20,51,53 ] All three nanofibers exhibited absorbance peaks at ≈2900, ≈1650, and ≈1400 cm −1 which were attributed to the CH 2 stretching, amide bond, and CH 2 bending vibration, respectively. It could be concluded that the nanofibrous web is composed of all chitosan, gelatin, and PVA compounds.…”

Section: Resultsmentioning

confidence: 99%

“…The contact angle of all samples was less than 90° which indicated their hydrophilicity and their cell affinity and surface cell recognition sites as result. [ 20 ] PVA, chitosan, and gelatin have a hydrophilic nature because of the existence of functional groups in their structure to form hydrogen bonding. [ 60 ] The PVA nanofibrous web was the smallest contact angle because of the existence of OH groups.…”

Section: Resultsmentioning

confidence: 99%

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Fabrication and DOE Optimization of Electrospun Chitosan/Gelatin/PVA Nanofibers for Skin Tissue Engineering

Hadipour-Goudarzi

Hemmatinejad

Shokrgozar

2023

Macro Materials & Eng

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Chitosan/gelatin‐based nanofibers display excellent biological performance in tissue engineering because of their biocompatible composition and nanofibrous structure with a high surface‐to‐volume ratio mimicking the native extracellular matrix. In this study, to save time and cost of experiments, a response surface methodology based on Box–Behnken design (BBD) is developed to predict the mean diameter of (chitosan:gelatin)/poly(vinyl alcohol) (PVA) nanofibers in three volume ratios of chitosan:gelatin by considering PVA percentage, applied voltage, and flow rate as input variables. The morphology and chemical composition of nanofibers are investigated through scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR), respectively. The optimum conditions to yield the minimum diameter of nanofibers with chitosan:gelatin ratios of 25:75, 50:50, and 75:25 are found and result in 165, 121, and 92 nm, respectively, which show good accordance with BBD estimated results. The tensile testing indicates that nanofibers containing higher ratio of chitosan:gelatin result in higher tensile stress and lower toughness and tensile strain. The water contact angle analysis (WCA) shows the appropriate hydrophilicity of crosslinked nanofibers. The MTT assay shows excellent cell viability and cell attachment of nanofibers for mouse fibroblast (L929) cells. The results indicate that optimum nanofibers are potent candidates for wound healing applications.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Fabrication and DOE Optimization of Electrospun Chitosan/Gelatin/PVA Nanofibers for Skin Tissue Engineering

Hadipour-Goudarzi

Hemmatinejad

Shokrgozar

2023

Macro Materials & Eng

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“…This finding was influenced by the hydrophilic properties of the gelatin and PVA polymers. However, increasing the amount of PVA in the composite scaffold results in a decrease in the ratio of hydrophilic to hydrophobic groups in the polymer blend, thereby enhancing the flexibility of the scaffold [ 49 ]. Hence, the contact angle testing of the fabricated gelatin–PVA hydrogels demonstrated that the hydrophilicity of GPVA5_GNP has the highest contact angle, as it contains the highest percentage of PVA in the hydrogel formulation.…”

Section: Discussionmentioning

confidence: 99%

Injectable Crosslinked Genipin Hybrid Gelatin–PVA Hydrogels for Future Use as Bioinks in Expediting Cutaneous Healing Capacity: Physicochemical Characterisation and Cytotoxicity Evaluation

et al. 2022

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The irregular shape and depth of wounds could be the major hurdles in wound healing for the common three-dimensional foam, sheet, or film treatment design. The injectable hydrogel is a splendid alternate technique to enhance healing efficiency post-implantation via injectable or 3D-bioprinting technologies. The authentic combination of natural and synthetic polymers could potentially enhance the injectability and biocompatibility properties. Thus, the purpose of this study was to characterise a hybrid gelatin–PVA hydrogel crosslinked with genipin (GNP; natural crosslinker). In brief, gelatin (GE) and PVA were prepared in various concentrations (w/v): GE, GPVA3 (3% PVA), and GPVA5 (5% PVA), followed by a 0.1% (w/v) genipin (GNP) crosslink, to achieve polymerisation in three minutes. The physicochemical and biocompatibility properties were further evaluated. GPVA3_GNP and GPVA5_GNP with GNP demonstrated excellent physicochemical properties compared to GE_GNP and non-crosslinked hydrogels. GPVA5_GNP significantly displayed the optimum swelling ratio (621.1 ± 93.18%) and excellent hydrophilicity (38.51 ± 2.58°). In addition, GPVA5_GNP showed an optimum biodegradation rate (0.02 ± 0.005 mg/h) and the highest mechanical strength with the highest compression modulus (2.14 ± 0.06 MPa). In addition, the surface and cross-sectional view for scanning electron microscopy (SEM) displayed that all of the GPVA hydrogels have optimum average pore sizes (100–199 μm) with interconnected pores. There were no substantial changes in chemical analysis, including FTIR, XRD, and EDX, after PVA and GNP intervention. Furthermore, GPVA hydrogels influenced the cell biocompatibility, which successfully indicated >85% of cell viability. In conclusion, gelatin–PVA hydrogels crosslinked with GNP were proven to have excellent physicochemical, mechanical, and biocompatibility properties, as required for potential bioinks for chronic wound healing.

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“…The results on the 7th day showed that the number of cells in the AD group continued to decline, while the number of cells in the BC group was larger. This difference may be due to the use of gelatin composite materials in group B, which is conducive to cell growth and clinging [27,28]; while group C has more groove structures than group A, which indicates that the introduced microstructure is conducive to cell growth; comparing group D samples with group B, there is less gelatin material, and the number of cells is significantly reduced, which indicates that the gelatin composite material has better biocompatibility. The results on the 14th day showed that the growth of the three groups of ACD cells was not as ideal as that of group B, which also verified the In addition, a macroscopic view of cell growth is shown in Figure 7.…”

Section: Cell Viabilitymentioning

confidence: 99%

New Method for Preparing Small-Caliber Artificial Blood Vessel with Controllable Microstructure on the Inner Wall Based on Additive Material Composite Molding

et al. 2021

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The diameter of most blood vessels in cardiovascular and peripheral vascular system is less than 6 mm. Because the inner diameter of such vessels is small, a built-in stent often leads to thrombosis and other problems. It is an important goal to replace it directly with artificial vessels. This paper creatively proposed a preparation method of a small-diameter artificial vascular graft which can form a controllable microstructure on the inner wall and realize a multi-material composite. On the one hand, the inner wall of blood vessels containing direct writing structure is constructed by electrostatic direct writing and micro-imprinting technology to regulate cell behavior and promote endothelialization; on the other hand, the outer wall of blood vessels was prepared by electrospinning PCL to ensure the stability of mechanical properties of composite grafts. By optimizing the key parameters of the graft, a small-diameter artificial blood vessel with controllable microstructure on the inner wall is finally prepared. The corresponding performance characterization experimental results show that it has advantages in structure, mechanical properties, and promoting endothelialization.

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Chitosan/gelatin/PVA membranes for mammalian cell culture

Cited by 11 publications

References 43 publications

Fabrication and DOE Optimization of Electrospun Chitosan/Gelatin/PVA Nanofibers for Skin Tissue Engineering

Fabrication and DOE Optimization of Electrospun Chitosan/Gelatin/PVA Nanofibers for Skin Tissue Engineering

Injectable Crosslinked Genipin Hybrid Gelatin–PVA Hydrogels for Future Use as Bioinks in Expediting Cutaneous Healing Capacity: Physicochemical Characterisation and Cytotoxicity Evaluation

New Method for Preparing Small-Caliber Artificial Blood Vessel with Controllable Microstructure on the Inner Wall Based on Additive Material Composite Molding

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