Chitosan–hyaluronan/nano chondroitin sulfate ternary composite sponges for medical use

Anisha, B.S.; Sankar, Deepthi; Mohandas, Annapoorna; Chennazhi, K.P.; Nair, Shantikumar V.; Jayakumar, R.

doi:10.1016/j.carbpol.2012.10.058

Cited by 115 publications

(40 citation statements)

References 23 publications

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“…This agent may support drug delivery system development, 36 bioprosthetic heart valve construction, 26 preparation of collagen scaffolds, 37 and a large number of other purposes. [38][39][40][41] However, these procedures use EDC to treat biomaterial structures prior to their contact with cells/tissues. The same is not observed when EDC is applied on dentin, since its fluid-filled tubular and porous structure allows EDC to diffuse across dentin to reach the odontoblast-like cells following its application.…”

Section: Discussionmentioning

confidence: 99%

Transdentinal Cytotoxicity of Carbodiimide (EDC) and Glutaraldehyde on Odontoblast-like Cells

Scheffel

Bianchi

Soares

et al. 2015

Operative Dentistry

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Carbodiimide (EDC) and glutaraldehyde are cross-linking agents capable of increasing resin-dentin bond durability. This study showed that these substances might be safely applied on acid-etched dentin since they have no transdentinal cytotoxic effects on cultured odontoblast-like cells. SUMMARYObjective: To evaluate the transdentinal cytotoxicity of three different concentrations of carbodiimide (EDC) or 5% glutaraldehyde (GA) on MDPC-23 cells.Methods: Seventy 0.4-mm-thick dentin disks obtained from human molars were adapted to artificial pulp chambers. MDPC-23 cells were seeded on the pulpal surface of the disks. After 48 hours, the occlusal dentin was acid-etched and treated for 60 seconds with one of the Results: EDC at all test concentrations did not reduce cell viability, while 5% GA did increase cell metabolism. Cell death by necrosis was not elicited by EDC or 5% GA. At the 24-hour period, 0.3 M and 0.5 M EDC reduced TP production by 18% and 36.8%, respectively. At seven days, increased TP production was observed in all groups. Collagen production at the 24-hour period was reduced when 0.5 M EDC was used. After seven days, no difference was observed among the groups. SEM showed no alteration in cell morphology or number, except in the hydrogen peroxide group.Conclusions: Treatment of acid-etched dentin with EDC or GA did not cause transdentinal cytotoxic effects on odontoblast-like cells.

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Section: Discussionmentioning

confidence: 99%

Transdentinal Cytotoxicity of Carbodiimide (EDC) and Glutaraldehyde on Odontoblast-like Cells

Scheffel

Bianchi

Soares

et al. 2015

Operative Dentistry

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“…Chitosan was also used in the formation of hydrogels based on the formation of complexes between chitosan/chondroitin sulphates (CT/CS) then applying as devices for chondroitin release [64]. Chitosan-hyaluroan composite cross-linked with chondroitin sulphate nanoparticles (CH-HYA/nCS) was developed based on simple ionic cross-linking using N,N-(3-dimethylaminopropyl)-Nethyl Carbodiimide (EDC) [65], followed by lyophilisation to obtain the composite sponge [66][67][68]. CH-HYA/nCS was structurally and physicochemically evaluated showing high porosity (60-70%).…”

Section: Pharmaceutical Strategies To Enhance the Delivery Of Glucosamentioning

confidence: 99%

“…It also showed high swelling and water uptake properties, owing to the high porosity of the nanocomposite sponges, with high biodegradation profile and cell viability, indicating that CH-HYA/nCS sponge was totally biocompatible. This sponge technique was recommended as a scaffold for chondroitin sulphate release [65].…”

Section: Pharmaceutical Strategies To Enhance the Delivery Of Glucosamentioning

confidence: 99%

Nutraceutical Formulations Containing Glucosamine and Chondroitin Sulphate in the Treatment of Osteoarthritis: Emphasis on Clinical Efficacy and Formulation Challenges

Agiba¹

2017

Int J Curr Pharm Sci

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Osteoarthritis (OA) is essentially a debilitating disease symptomatized by a gradual loss of articular joint cartilage, causing painful impairment among the population of different ages, particularly patients over the age of 50 y. Nutraceuticals; namely glucosamine and chondroitin have been widely used in the treatment of OA. The chondroprotective properties of the aforementioned agents have been reported, allowing the repair and recovery of the articular surface in OA. The purpose of this review article is to report the current evidence for the use of glucosamine and chondroitin sulphate in the treatment of knee OA with emphasis on their indications for clinical use, effectiveness and safety. It also highlights the role of some advanced formulation technologies in optimizing the delivery of those drugs.

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“…In this contest, the importance of a porous, 3D scaffold with native extracellular matrix (ECM) mimicking components come into the picture. Chitin, the second most abundant natural biopolymer after collagen, has been widely exploited due to its ECM-mimicking nature and one of the degradation products, viz, N-acetyl-D-glucosamine, has been reported to promote fibroblast proliferation and ordered collagen deposition [1]. Chitin also promotes macrophage migration and stimulates the deposition of granulation tissue.…”

Section: Introductionmentioning

confidence: 99%

“…To prove this hypothesis, we mixed chondroitin sulfate nanoparticles with the chitin/PBS composite. Chondroitin sulfate nanoparticles have been proven, in our previous report, to provide an enhanced fibroblast proliferation [1]. The developed ternary composite was then characterized and analyzed for cytocompatibility using Human Dermal Fibroblasts (HDF).…”

Section: Introductionmentioning

confidence: 99%

Fabrication of Chitin/Poly(butylene succinate)/Chondroitin Sulfate Nanoparticles Ternary Composite Hydrogel Scaffold for Skin Tissue Engineering

et al. 2014

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Skin loss is one of the oldest and still not totally resolved problems in the medical field. Since spontaneous healing of the dermal defects would not occur, the regeneration of full thickness of skin requires skin substitutes. Tissue engineering constructs would provide a three dimensional matrix for the reconstruction of skin tissue and the repair of damage. The aim of the present work is to develop a chitin based scaffold, by blending it with poly(butylene succinate) (PBS), an aliphatic, biodegradable and biocompatible synthetic polymer with excellent mechanical properties. The presence of chondroitin sulfate nanoparticles (CSnp) in the scaffold would favor cell adhesion. A chitin/PBS/CSnp composite hydrogel scaffold was developed and characterized by SEM (Scanning Electron Microscope), FTIR (Fourier Transform Infrared Spectroscopy), and swelling ratio of scaffolds were analyzed. The scaffolds were evaluated for the suitability for skin tissue engineering application by cytotoxicity, cell attachment, and cell proliferation studies using human dermal fibroblasts (HDF). The cytotoxicity and cell proliferation studies using HDF confirm the suitability of the scaffold for skin regeneration. In short, these results show OPEN ACCESSPolymers 2014, 6 2975 promising applicability of the developed chitin/PBS/CSnps ternary composite hydrogel scaffolds for skin tissue regeneration.

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Chitosan–hyaluronan/nano chondroitin sulfate ternary composite sponges for medical use

Cited by 115 publications

References 23 publications

Transdentinal Cytotoxicity of Carbodiimide (EDC) and Glutaraldehyde on Odontoblast-like Cells

Transdentinal Cytotoxicity of Carbodiimide (EDC) and Glutaraldehyde on Odontoblast-like Cells

Nutraceutical Formulations Containing Glucosamine and Chondroitin Sulphate in the Treatment of Osteoarthritis: Emphasis on Clinical Efficacy and Formulation Challenges

Fabrication of Chitin/Poly(butylene succinate)/Chondroitin Sulfate Nanoparticles Ternary Composite Hydrogel Scaffold for Skin Tissue Engineering

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