Chitosan Hydrogel Dressing Loaded with Adipose Mesenchymal Stem Cell-Derived Exosomes Promotes Skin Full-Thickness Wound Repair

Abstract

Cutaneous trauma repair is still a challenge in the clinic due to the scar formation and slow healing rate, especially for diabetic patients. Various drug-loading wound dressings have been explored to solve this problem. Mesenchymal stem cell (MSC)derived exosomes have been considered as a potential cell-free drug because of their anti-inflammation function and tissue repair property that are comparable to that of MSCs. Herein, a composite wound dressing (Exo/Gel) consisting of the chitosan hydrogel and adipose MSC-derived exosome (ADMSC−Exo) was designed and fabricated by a physical mixing method to promote the skin fullthickness wound repair. The exosomes were slowly released from the Exo/Gel dressing with the degradation of the chitosan hydrogel. The Exo/Gel displayed enhanced cell migration and angiogenic properties in vitro. And the results in the rat skin wound model showed that the Exo/Gel could promote the regular collagen deposition, angiogenesis, and hair follicle mosaicism regeneration. These results proved that the hydrogel dressing with ADMSCs-derived exosomes can accelerate skin wound healing, which is a strategy for developing wound dressings.

Exosomes derived from mesenchymal stem cells in diabetes and diabetic complications

“…Adipose tissue contains a larger concentration of MSCs compared to bone marrow and other sources. Obtaining exosomes from adipose stem cells is less invasive and lacks ethical limits when compared to bone marrow stem cells, as well as immunomodulatory properties [ 21 ]. Additionally, UCMSCs derived exosomes are extracted from discarded umbilical cords.…”

Smart responsive in situ hydrogel systems applied in bone tissue engineering

“…The ideal candidate biomaterials can bridge tissue damage and improve regeneration by enhancing new natural tissue regeneration ( Xue et al, 2021 ). Simultaneously, they are also required to demonstrate excellent biocompatible, tunable mechanical properties and biodegradation as they degrade gradually during tissue repair ( Wu D. et al, 2024 ; Zhou H. et al, 2024 ; Vaidya et al, 2024 ). To date, a variety of natural and synthetic biomaterials have been applied in an attempt to simulate ECM of the natural bone tissue ( Xu et al, 2023 ; Sung et al, 2024 ; Yoon et al, 2024 ).…”