Chitosan hydrogel nanoparticle enhance therapeutic effect of bovine umbilical mesenchymal stem cell conditioned medium on canine cognitive dysfunction or canine alzheimer’s like mediated by inhibition of neuronal apoptotic

Abstract

In treating brain diseases, such as canine cognitive dysfunction, most currently available potent drugs have weak therapeutic efficacy. One of the causes is the inability of the substance to reach the brain in therapeutic quantities. These pharmaceuticals lacked targeted mechanisms for drug delivery, coming about in an elevated drugs concentration in imperative organs, which driven to drug harmfulness. In recent years, cell-free treatment (conditioned medium) determined from animal and human stem cells have provided new promise for treating brain diseases, as CM can stimulate the regeneration of neurons and prevent the inflammation and apoptotic of neurons caused by pathology or aging. On the other hand, it is well known that Chitosan-Hydrogel (CH) is a polymer derived from natural sources. It has been authorized for use in biomedical use because of its uncommon biodegradability, biocompatibility, and mucoadhesive properties. CH modification has been utilized to generate nanoparticles (NPs) for intranasal and intravenous brain targeting. NPs shown upgraded drug take-up to the brain with decreased side impacts due to their drawn out contact time with the nasal mucosa, surface charge, nano size, and capacity to extend the tight intersections inside the mucosa. Due to the aforementioned distinctive characteristics, developing CHNPs load with Bovine Umbilical Mesenchymal Stem Cell Conditioned Medium is crucial as a new therapeutic strategy for canine cognitive dysfunction.

Smart responsive in situ hydrogel systems applied in bone tissue engineering

“…To achieve the various smart responses, the various natural or synthetic hydrogels are usually modified by functional groups (e.g., unsaturated bond) or compounded with functional nanoparticles (e.g., MoS 2 , Cu 2 O, F 3 O 4 , etc.) ( Faustino et al, 2023 ; Wihadmadyatami et al, 2023 ; Hong et al, 2024 ).…”