Chitosan-<i>g</i>-oligo(L,L-lactide) Copolymer Hydrogel Potential for Neural Stem Cell Differentiation

Revkova, V. A.; Grebenik, Ekaterina A.; Kalsin, Vladimir A.; Демина, Т. С.; Bardakova, Kseniia N.; Shavkuta, Boris; Melnikov, Pavel; Samoilova, E. M.; Konoplyannikov, Mikhail; Efremov, Yuri M.; Zhang, Chao; Акопова, Т. А.; Troitsky, Alexandr V; Timashev, Peter; Baklaushev, Vladimir P.

doi:10.1089/ten.tea.2019.0265

Cited by 21 publications

(18 citation statements)

References 44 publications

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“…Indeed, in matrigel, in the medium containing BDNF and GDNF, in 2 weeks, almost all drNPCs undergo differentiation, characterizing by the disappearance of SOX2 and formation of a NF-200-positive neuronal network and GFAP-positive astrocytes. 7 …”

Section: Discussionmentioning

confidence: 99%

“…An interesting finding of this study is the ability of SBEM, both unmodified and peptide-modified, to provide the preservation of undifferentiated drNPCs even in the differentiation medium. Indeed, in matrigel, in the medium containing BDNF and GDNF, in 2 weeks, almost all drNPCs undergo differentiation, characterizing by the disappearance of SOX2 and formation of a NF-200-positive neuronal network and GFAP-positive astrocytes …”

Section: Discussionmentioning

confidence: 99%

“…The development of ECM-mimicking biomaterials has made it possible to create organotypic 3D cultures for studying directed differentiation, migration, and intercellular cooperation of neuroglial cells. 6 , 7 …”

Section: Introductionmentioning

confidence: 99%

“…At the same time, their interaction with the microenvironment and specifics of 3D culturing are understood poorer than those of iPSC derivatives. The development of ECM-mimicking biomaterials has made it possible to create organotypic 3D cultures for studying directed differentiation, migration, and intercellular cooperation of neuroglial cells. , …”

Section: Introductionmentioning

confidence: 99%

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Spidroin Silk Fibers with Bioactive Motifs of Extracellular Proteins for Neural Tissue Engineering

et al. 2021

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The interaction of neural progenitor cells (NPCs) with the extracellular matrix (ECM) plays an important role in neural tissue regeneration. Understanding which motifs of the ECM proteins are crucial for normal NPC adhesion, proliferation, and differentiation is important in order to create more adequate tissue engineered models of neural tissue and to efficiently study the central nervous system regeneration mechanisms. We have shown earlier that anisotropic matrices prepared from a mixture of recombinant dragline silk proteins, such as spidroin 1 and spidroin 2, by electrospinning are biocompatible with NPCs and provide good proliferation and oriented growth of neurites. This study objective was to find the effects of spidroin-based electrospun materials, modified with peptide motifs of the extracellular matrix proteins (RGD, IKVAV, and VAEIDGIEL) on adhesion, proliferation, and differentiation of directly reprogrammed neural precursor cells (drNPCs). The structural and biomechanical studies have shown that spidroin-based electrospun mats (SBEM), modified with ECM peptides, are characterized by a uniaxial orientation and elastic moduli in the swollen state, comparable to those of the dura mater. It has been found for the first time that drNPCs on SBEM mostly preserve their stemness in the growth medium and even in the differentiation medium with brain-derived neurotrophic factor and glial cell line-derived neurotrophic factor, while addition of the mentioned ECM-peptide motifs may shift the balance toward neuroglial differentiation. We have demonstrated that the RGD motif promotes formation of a lower number of neurons with longer neurites, while the IKVAV motif is characterized by formation of a greater number of NF200-positive neurons with shorter neurites. At the same time, all the studied matrices preserve up to 30% of neuroglial progenitor cells, phenotypically similar to radial glia derived from the subventricular zone. We believe that, by using this approach and modifying spidroin by various ECM-motifs or other substances, one may create an in vitro model for the neuroglial stem cell niche with the potential control of their differentiation.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Spidroin Silk Fibers with Bioactive Motifs of Extracellular Proteins for Neural Tissue Engineering

et al. 2021

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“…Owing to their pronounced amphiphilic properties the copolymers can be used as emulsifiers for biodegradable microparticles with a complex architecture by solvent evaporation from oil/water emulsions [104,105]. A higher hydrophobization of chitosan, as compared to modification by interaction with lactide, made it possible to use copolymers to obtain hydrogels of a given architecture by stereolithography; the hydrogels were successfully used as scaffolds for nerve tissue regeneration [103,106,107]. The grafting of short and medium chains of oligolactides on chitosan gives it new properties but, in general, does not provide the necessary hydrophobicity.…”

Section: Copolymers Of Chitosan With Polylactide and Materialsmentioning

confidence: 99%

Materials Based on Chitosan and Polylactide: From Biodegradable Plastics to Tissue Engineering Constructions

2021

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The transition to green chemistry and biodegradable polymers is a logical stage in the development of modern chemical science and technology. In the framework of this review, the advantages, disadvantages, and potential of biodegradable polymers of synthetic and natural origin are compared using the example of polylactide and chitosan as traditional representatives of these classes of polymers, and the possibilities of their combination via obtaining composite materials or copolymers are assessed. The mechanochemical approach to the synthesis of graft copolymers of chitosan with oligolactides/polylactides is considered in more detail.

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Review: the latest advances in biomedical applications of chitosan hydrogel as a powerful natural structure with eye-catching biological properties

et al. 2022

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Chitosan-g-oligo(L,L-lactide) Copolymer Hydrogel Potential for Neural Stem Cell Differentiation

Cited by 21 publications

References 44 publications

Spidroin Silk Fibers with Bioactive Motifs of Extracellular Proteins for Neural Tissue Engineering

Spidroin Silk Fibers with Bioactive Motifs of Extracellular Proteins for Neural Tissue Engineering

Materials Based on Chitosan and Polylactide: From Biodegradable Plastics to Tissue Engineering Constructions

Review: the latest advances in biomedical applications of chitosan hydrogel as a powerful natural structure with eye-catching biological properties

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