Chitosan Is Necessary for the Structure of the Cell Wall, and Full Virulence of Ustilago maydis

Abstract

Smut fungi comprise a large group of biotrophic phytopathogens infecting important crops, such as wheat and corn. U. maydis is a plant pathogenic fungus responsible for common smut in maize and teocintle. Through our analysis of the transcriptome of the yeast-to-mycelium dimorphic transition at acid pH, we determined the number of genes encoding chitin deacetylases of the fungus, and observed that the gene encoding one of them (UMAG_11922; CDA1) was the only one up-regulated. The mutation of this gene and the analysis of the mutants revealed that they contained reduced amounts of chitosan, were severely affected in their virulence, and showed aberrant mycelial morphology when grown at acid pH. When the CDA1 gene was reinserted into the mutants by the use of an autonomous replication plasmid, virulence and chitosan levels were recovered in the retro mutant strains, indicating that the CDA1 gene was involved in these features. These data revealed that chitosan plays a crucial role in the structure and morphogenesis of the cell wall during mycelial development of the fungus, and that in its absence, the cell wall becomes altered and is unable to support the stress imposed by the defense mechanism mounted on by the plant host during the infection process.

Effect of Shock Waves on the Growth of Aspergillus niger Conidia: Evaluation of Germination and Preliminary Study on Gene Expression

“…The cell wall of filamentous fungi is an essential component for morphogenesis and cell viability as well as protection against external ambient stress factors [48][49][50]. Its structure is a physically rigid layer formed mainly of networks of carbohydrates (β-1,3 glucan, β-1,6 glucan, and chitin), proteins (mannoproteins and hydrophobins), and other surface components, such as melanins [7,51].…”

Effect of Shock Waves on the Growth of <em>Aspergillus niger</em> Conidia: Evaluation of Germination and Preliminary Study on Gene Expression

“…The cell wall of filamentous fungi is an essential component for morphogenesis and cell viability as well as protection against external ambient stress factors [45][46][47]. Its structure is a physically rigid layer formed mainly of networks of carbohydrates (β-1,3 glucan, β-1,6 glucan, chitin), proteins (mannoproteins, hydrophobins), and other surface components, such as melanins [7,48].…”