Chitosan‐laponite nanocomposite scaffolds for wound dressing application

Abstract

The pivotal issue of skin regeneration research is the development of effective biomaterials that exhibit biological activities as fungicide and bactericide, combining simple and low cost manufacturing technologies. In this context, nanocomposite scaffolds based on chitosan (Ch)/Laponite (Lap) were produced by using different concentrations of Lap via freeze‐drying process for potential application in skin regeneration. The influence of Lap concentration on the scaffold properties was evaluated. The prepared scaffolds were characterized by x‐ray diffraction (XRD), scanning electron microscopy (SEM), porosity, swelling capacity, and mechanical analyses. The results revealed that the scaffolds exhibited a porous architecture, besides the increase in the clay content, leads to an increase in the porosity, an improvement of mechanical strength, and a decrease of swelling capacity. In vitro tests were also carried out to evaluate the biocompatibility of the materials, such as bioadhesion, antibacterial activity, viability, and cell adhesion. Viability and cell adhesion demonstrated that all scaffolds were not cytotoxic and the fibroblast cells readily attached on the surface of the scaffolds. Thereby, the results suggested that the nanocomposite scaffolds are biomaterials potentially useful as wound dressings.

Immobilization of carboxymethyl chitosan/laponite on polycaprolactone nanofibers as osteoinductive bone scaffolds

“…The viability of cells on PCL and PCL@CMC scaffolds was also assessed for the comparison. Results showed that the cell viability was significantly improved from PCL to PCL@CMC and PCL@CMC/LAP3.5% during this time (Figure 3B) Gonzaga et al reported that the addition of LAP nanoplatelets to chitosan enhanced the cell proliferation on the corresponding scaffolds 24 …”

Polylactide/Polyvinylalcohol-Based Porous Bioscaffold Loaded with Gentamicin for Wound Dressing Applications

“…The porosity of the bioscaffolds was determined by liquid displacement method [35]. The weight (W 1 ) and volume (V) of bioscaffolds were measured before the immersion into ethanol.…”

“…After the saturation of bioscaffolds by the absorption of ethanol, the weight of bioscaffolds were measured again (W 2 ). The porosity of the bioscaffolds was calculated according to the Equation (2) (ρ represents the density of the ethanol) [35]. All experiments were performed in triplicate.…”

“…The degree of swelling is mainly dependent on the porosity and hydrophilicity of the bioscaffolds [35,61]. This property of the bioscaffolds plays an important role in the acceleration of wound healing as it absorbs exudates and fluids secreted from the wound and provides a moist environment for the wound area [3,8].…”

Steady and Oscillatory Shear Flow Behavior of Different Polysaccharides with Laponite®

“…Laponite ® was also processed with chitosan to develop a scaffold for wound healing applications. The material had an increase in porosity and an improvement in the mechanical properties after the addition of the clay [17]. Laponite ® was added to a gellan gum's injectable hydrogel to confer stability and strength to the obtained material [18].…”