Chitosan microcapsules as controlled release systems for insulin

Aiedeh, Khaled; Gianasi, Elisabetta; Orienti, Isabella; Zecchi,

doi:10.3109/02652049709006810

Cited by 121 publications

(47 citation statements)

References 17 publications

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“…The significant increase may be because of the increase in the viscosity of the droplets (due to the increase in concentration of polymer solution). This increase is high enough to result in difficult dispersion and subdivision of droplets reported (21). Increase in MPS due to increased viscosity of the polymer solution has also been reported (22,23).…”

Section: Particle Size Distributionmentioning

confidence: 79%

Evaluation of Ionotropic Cross-Linked Chitosan/Gelatin B Microspheres of Tramadol Hydrochloride

Basu

Kavitha²,

M³

2010

AAPS PharmSciTech

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Abstract. Microspheres of tramadol hydrochloride (TM) for oral delivery were prepared by complex coacervation method without the use of chemical cross-linking agents such as glutaraldehyde to avoid the toxic reactions and other undesirable effects of the chemical cross-linking agents. Alternatively, ionotropic gelation was employed by using sodium-tripolyphosphate as cross-linking agent. Chitosan and gelatin B were used as polymer and copolymer, respectively. All the prepared microspheres were subjected to various physicochemical studies, such as drug-polymer compatibility by thin layer chromatography (TLC) and Fourier transform infrared (FTIR) spectroscopy, surface morphology by scanning electron microscopy, frequency distribution, drug entrapment efficiency, in vitro drug release characteristics and release kinetics. The physical state of drug in the microspheres was determined by differential scanning calorimetry (DSC) and X-ray diffractometry (XRD). TLC and FTIR studies indicated no drug-polymer incompatibility. All the microspheres showed initial burst release followed by a fickian diffusion mechanism. DSC and XRD analysis indicated that the TM trapped in the microspheres existed in an amorphous or disordered-crystalline status in the polymer matrix. From the preliminary trials, it was observed that it may be possible to formulate TM microspheres by using biodegradable natural polymers such as chitosan and gelatin B to overcome the drawbacks of TM and to increase the patient compliance.

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Section: Particle Size Distributionmentioning

confidence: 79%

Evaluation of Ionotropic Cross-Linked Chitosan/Gelatin B Microspheres of Tramadol Hydrochloride

Basu

Kavitha²,

M³

2010

AAPS PharmSciTech

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“…So far there are no delivery systems for foods which are designed to enhance the efficiency and stability of bioactive agents [15].…”

Section: Introductionmentioning

confidence: 99%

Digestion characteristics and kinetic analysis of bio-molecules in a simulated human intestinal system

Choi¹,

Buisson²,

Kim³

2015

Integr Food Nutr Metab

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We designed and assembled a simulated human intestinal system (SHIS) consisting of a stomach and a small intestine. Each reactor vessel has several ports such as the input and output of medium, a sampler of liquid phase, a pH electrode, a pH control (acid and base), and a thermometer. The SHIS was kept at body temperature (37°C) by pumping water into the space between the jacket and the inside walls. The stomach chamber was initially filled with gas fluids and then digested for 2 hr. After the stomach digestion, the residuals was delivered from stomach chamber into the small intestine chamber by secreting intestinal fluid for 4 hr, followed by secretion of 0.3M NaHCO 3 , 0.1M NaHCO 3 , 4% bile salt, and 2% bile salt for 1, 3, 0.5, and 3.5 hrs, respectively. In order to prepare the alginate/chitosan capsule as a delivery system for bioactive agents, the microencapsulation system was assembled with a control unit, a electrical, a pneumatic systems, and a reaction vessel. After digestion of alginate capsules, the content of total sugar was 7.47% and 60.82% in the stomach and small intestine (simulated human intestinal system, SHIS), respectively. However, in case of alginate/chitosan capsule and alginate/chitosan capsule coated with polyacryl emulsion (alginate/chitosan-PAE), the total sugar content were 3.12 and 4.62% in the stomach model and 43.46 and 42.09% in the small intestine model, respectively. There were no difference on the degree of digestion in the alginate capsule and alginate/polyethylene glycol (alginate/PEG) capsule prepared with 0.1-0.3% of PEG in the stomach model. As a result, the alginate matrix remained in a shrunken state due to conversion of sodium alginate into insoluble alginic acid, which acted as a barrier to chitosan microparticles. And it was effective for digesting by intestinal fluid and releasing of the sugar.

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“…Because chitosan shares some characteristics with glycosaminoglycans (GAG) and hyaluronic acid, it is used in various tissue engineering applications (Suh and Matthew 2000). In addition, the biodegradabe, biocompatible, non-antigenic, non-toxic and bifunctional properties of chitosan make this polymer a suitable material for biomedical applications such as wound dressings (Hirano et al 1990) and drug delivery (Aiedeh et al 1997). It is known that chitosan plays a role in cell attachment and proliferation (Zhu et al 2002).…”

Section: Introductionmentioning

confidence: 99%

A cost-effective and simple culture method for primary hepatocytes

Aday

Hasırcı

Gürhan

2011

Animal Cells and Systems

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Hepatocytes, the major epithelial cells of the liver, maintain their morphology in culture dishes coated with extracellular matrix (ECM) components such as collagen and fibronectin or biodegradable polymers (e.g. chitosan, gelatin). In these coated dishes, survival of cells and maintaining of liver-specific functions may increase. The aim of this study was to determine a suitable, cost-effective and simple system for hepatocyte isolation and culture which may be useful for various applications such as in vitro toxicology studies, hepatocyte transplantation and bioartificial liver (BAL) systems. In order to obtain primary cultures, hepatocytes were isolated from liver by an enzymatic method and cultured on plates coated with collagen, chitosan or gelatin. Collagen, gelatin-sandwich and gelatin-cell mixture methods were also evaluated. Morphology and attachment of the cells were observed by inverted microscope and scanning electron microscope (SEM). An MTT assay was used to determine cell viability and mitochondrial activity.

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Chitosan microcapsules as controlled release systems for insulin

Cited by 121 publications

References 17 publications

Evaluation of Ionotropic Cross-Linked Chitosan/Gelatin B Microspheres of Tramadol Hydrochloride

Evaluation of Ionotropic Cross-Linked Chitosan/Gelatin B Microspheres of Tramadol Hydrochloride

Digestion characteristics and kinetic analysis of bio-molecules in a simulated human intestinal system

A cost-effective and simple culture method for primary hepatocytes

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